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不同强度的低强度脉冲超声波对椎间盘退变的影响
Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice
- 第三军医大学学报 2018年40卷第22期 页码：2027-2033
- 作者机构：
1.400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)：战创伤康复研究室，创伤实验室, 骨代谢与修复中心, 创伤、烧伤与复合伤国家重点实验室
2.400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)：康复医学科
- 作者简介：
[ 陈林，E-mail: linchen70@163.com CHEN Lin, E-mail: linchen70@163.com
- 基金信息：科技部重大研究计划(2014CB942904);军事医学创新工程重点项目(16CXZ016)
DOI：10.16016/j.1000-5404.201809052
中图分类号： R-332; R454.3; R681.5收稿：2018-09-06，
修回：2018-09-18，
纸质出版：2018-11-30
- 稿件说明：
移动端阅览
蒋宛凌, 黄俊兰, 谭乔燕, 等. 不同强度的低强度脉冲超声波对椎间盘退变的影响[J]. 第三军医大学学报, 2018,40(22):2027-2033.
Wanling JIANG, Junlan HUANG, Qiaoyan TAN, et al. Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2027-2033.
蒋宛凌, 黄俊兰, 谭乔燕, 等. 不同强度的低强度脉冲超声波对椎间盘退变的影响[J]. 第三军医大学学报, 2018,40(22):2027-2033. DOI： 10.16016/j.1000-5404.201809052.
Wanling JIANG, Junlan HUANG, Qiaoyan TAN, et al. Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2027-2033. DOI： 10.16016/j.1000-5404.201809052.
摘要
目的
2
探讨不同强度的低强度脉冲超声波对椎间盘退变的影响，筛选低强度脉冲超声波的最佳治疗强度。
方法
2
采用3种强度(0.1、0.5、1.0 W/cm
2
)的低强度脉冲超声波处理大鼠髓核细胞及筛选的最佳强度超声波处理针刺椎间盘模型小鼠；EdU检测髓核细胞增殖，划痕实验检测细胞迁移，定量PCR检测蛋白聚糖、Ⅱ型胶原、波形蛋白等相关基因表达；组织学分析小鼠尾部椎间盘退变程度。
结果
2
3种强度的低强度脉
冲超声波能促进髓核细胞增殖及迁移，强度为0.5 W/cm
2
及1.0 W/cm
2
的低强度脉冲超声波作用较明显(
P
<
0.01)。低强度脉冲超声波也可上调正常环境及炎症刺激下髓核细胞中蛋白聚糖、波形蛋白等表达，其中强度为0.5 W/cm
2
、频率为3 MHz、占空比为50%中强度的低强度脉冲超声波作用最强(
P
<
0.01)。对照组小鼠椎间盘表现出明显退变表型，而超声组椎间盘退变程度的组织评分明显低于对照组(
P
<
0.01)。
结论
2
低强度脉冲超声波可能通过上调椎间盘的蛋白聚糖、波形蛋白、Ⅱ型胶原表达，加快细胞增殖及迁移等，对椎间盘退变有一定防治作用，其中强度为0.5 W/cm
2
、频率为3 MHz、占空比为50%中强度的低强度脉冲超声波作用最明显。
Abstract
Objective
2
To investigate the effect of low-intensity pulsed ultrasound (LIPUS) at different intensities on intervertebral disc degeneration (IDD) and determine the optimal therapeutic intensity.
Methods
2
LIPUS at the intensities of 0.1
0.5 and 1.0 W/cm
2
were applied on rat nucleus pulposus (NP) cells
and the optimal therapeutic intensity LIPUS was performed in a mouse model of intervertebral disc punctures. The cell proliferation and migration were detected by EdU and wound healing assays
respectively
and the expression levels of the related genes were examined using real-time quantitative PCR. Histological analysis was performed to evaluate the degeneration of the intervertebral discs in the mouse model after LIPUS treatment.
Results
2
LIPUS treatments at the 3 intensities all promoted the proliferation and migration of rat NP cells
but the effect was stronger with LIPUS intensities of 0.5 and 1.0 W/cm
2
(
P
<
0.01). The treatments up-regulated the mRNA expression levels of Aggrecan and Vimentin in rat NP cells
and the effect was the most obvious at the intensity of 0.5 W/cm
2
(frequency of 3 MHz with a duty cycle of 50%;
P
<
0.01). In LIPUS-treated mouse models of disc punctures
histological scoring showed a significantly lower score for IDD than that in untreated mice (
P
<
0.01).
Conclusion
2
LIPUS can ameliorate IDD by up-regulating the expressions of Aggrecan
Vimentin and type Ⅱ collagen to promote the proliferation and migration of NP cells. An intensity of 0.5 W/cm
2
a frequency of 3 MHz and a duty cycle of 50% can be the optimal LIPUS setting for treatment of IDD.
关键词
Keywords
references
YELIN E. Cost of musculoskeletal diseases: impact of work disability and functional decline[J]. J Rheumatol Suppl, 2003, 68: 8-11.
CHILDS J D, CLELAND J A, ELLIOTT J M, et al . Neck pain: Clinical practice guidelines linked to the International Classification of Functioning, Disability, and Health from the Orthopedic Section of the American Physical Therapy Association[J ] . J Orthop Sports Phys Ther, 2008, 38(9): A1-A34.
冯若, 王智彪.实用超声治疗学[M].北京:科学技术文献出版社, 2002: 1-247.
FENG R, WANG Z B. Practical ultrasound therapy[M]. Beijing: Science and Technical Documentation Press, 2002: 1-247.
OH C D, IM H J, SUH J, et al . Rho-associated kinase inhibitor immortalizes rat nucleus pulposus and annulus fibrosus cells: establishment of intervertebral disc cell lines with novel approaches[J ] . Spine, 2016, 41(5): E255-E261. DOI: 10.1097/BRS.0000000000001235.
[HWANG P Y, GILCHRIST C L, Francisco A T, et al . Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand[C ] . ASME 2012 Summer Bioengineering Conference, Parts A and B, 2012. DOI: 10.1115/sbc2012-80338 http://dx.doi.org/10.1115/sbc2012-80338 ..
TAM V, CHAN W C W, Leung V Y L, et al . Histological and reference system for the analysis of mouse intervertebral disc[J ] . J Orthop Res, 2018, 36(1): 233-243. DOI: 10.1002/jor.23637.
MANIADAKIS N, GRAY A. The economic burden of back pain in the UK[J]. Pain, 2000, 84(1): 95-103. DOI: 10.1016/s0304-3959(99)00187-6.
黄晓琳, 燕铁斌.康复医学[M].北京:人民卫生出版社, 2018.
HUANG X L, YAN T B. Rehabilitation medicine[M]. Beijing: People's Medical Publishing House, 2018.
ZHANG F, ZHAO X, SHEn H, et al . Molecular mechanisms of cell death in intervertebral disc degeneration (Review)[J ] . Int J Mol Med, 2016, 37(6): 1439-1448. DOI:10.3892/ijmm.2016.2573.
PODICHETTY V K. The aging spine: the role of inflammatory mediators in intervertebral disc degeneration[J]. Cell Mol Biol (Noisy-le-Grand), 2007, 53(5): 4-18.
VO N V, HARTMAN R A, PATIL P R, et al . Molecular mechanisms of biological aging in intervertebral discs[J ] . J Orthop Res, 2016, 34(8): 1289-1306. DOI:10.1002/jor.23195.
ROBERTSON V J, BAKER K G. A review of therapeutic ultrasound: effectiveness studies[J]. Phys Ther, 2001, 81(7): 1339-1350. DOI:10.1097/jwh.0b013e31820089ba.
陈景藻.现代物理治疗学[M].北京:人民军医出版社, 2001.
CHEN J Z. Modern physiotherapy[M]. Beijing: Military Science Publishing House, 2001.
LOW J, BAZIN S, DOCKER M, et al . Guide lines for the safe use of ultraviolet therapy equipment[J ] . Physiotherapy, 1994, 80(2): 89-90. DOI:10.1016/s0031-9406(10)61018-8.
ROTHENBERG J B, JAYARAM P, NAQVI U, et al . The role of low-intensity pulsed ultrasound on cartilage healing in knee osteoarthritis: a review[J ] . PM R, 2017, 9(12): 1268-1277. DOI:10.1016/j.pmrj.2017.05.008.
GAO G, HE J, NONG L, et al . Periodic mechanical stress induces the extracellular matrix expression and migration of rat nucleus pulposus cells by upregulating the expression of intergrin α1 and phosphorylation of downstream phospholipase Cγ1[J ] . Mol Med Rep, 2016, 14(3): 2457-2464. DOI: 10.3892/mmr.2016.5549.
IWABUCHI S, ITO M, HATA J, et al . In vitro evaluation of low-intensity pulsed ultrasound in herniated disc resorption[J ] . Biomaterials, 2005, 26(34): 7104-7114. DOI: 10.1016/j.biomaterials.2005.05.004..
CHEN M H, SUN J S, LIAO S Y, et al . Low-intensity pulsed ultrasound stimulates matrix metabolism of human annulus fibrosus cells mediated by transforming growth factor β1 and extracellular signal-regulated kinase pathway[J ] . Connect Tissue Res, 2015, 56(3): 219-227. DOI: 10.3109/03008207.2015.1016609.
SOBCZAK S, DUGAILLY P M, GILBERT K K, et al . Reliability and validation of in vitro lumbar spine height measurements using musculoskeletal ultrasound: a preliminary investigation[J ] . J Back Musculoskelet Rehabil, 2016, 29(1): 171-182. DOI: 10.3233/BMR-150613..
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