亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成

樊莉; 李聃丹; 田竞; 孙凤军; 冯伟; 周世文

doi:10.16016/j.1000-5404.201808141

您当前的位置：

首页 >

文章列表页 >

亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成

基础医学 | 更新时间：2026-01-09

- 亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成
- Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili
- 第三军医大学学报 2018年40卷第21期页码：1967-1972
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201808141
  中图分类号：
- 收稿：2018-08-21，
  
  纸质出版：2018-11-15
- 稿件说明：
移动端阅览
樊莉, 李聃丹, 田竞, 等. 亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成[J]. 第三军医大学学报, 2018,40(21):1967-1972.

Li FAN, Dandan LI, Jing TIAN, et al. Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili[J]. Journal of Third Military Medical University, 2018, 40(21): 1967-1972.
樊莉, 李聃丹, 田竞, 等. 亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成[J]. 第三军医大学学报, 2018,40(21):1967-1972. DOI： 10.16016/j.1000-5404.201808141.

Li FAN, Dandan LI, Jing TIAN, et al. Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili[J]. Journal of Third Military Medical University, 2018, 40(21): 1967-1972. DOI： 10.16016/j.1000-5404.201808141.

摘要

Abstract

Objective

To investigate the changes in the biofilm formation of imipenem-resistant and sensitive

Acinetobacter baumannii (A. baumannii)

isolates under the sub-minimal inhibitory concentration of imipenem (sub-MIC IMP).

Methods

MIC values were detected by agar plate dilution in both imipenem-resistant and

sensitive

A. baumannii

isolates. The carbapenemase resistance genes were amplified by PCR. After the intervention of sub-MIC IMP

the biofilm formation was detected by micro-well method

the bacterial adhesion was detected by colony plate counting

and the bacterial motility was observed by swimming

and the expression of the bacterial type IV pili gene was detected by quantitative RT-PCR.

Results

The results of carbapenemase resistance genes showed there were 10 strains of IMP-resistant

A. baumannii

carrying OXA-51 gene

9 carrying OXA-23 gene and only 3 IMP-4 gene. Sub-MIC IMP significantly induced the biofilm formation

adhesion and motility of IMP-resistant

A. baumannii

but no such changes were found in above aspects in the IMP-sensitive strains (5 strains exhibited inhibitory effects

3 showed no effect

and 2 presented induction). Enhanced expression level of type Ⅳ pili gene was found in all the strains with sub-MIC IMP induced biofilm formation.

Conclusion

Sub-MIC IMP may significantly enhance the biofilm formation of

A. baumannii

by affecting the Ⅳ pili related adherence and motility.

关键词

Keywords

references

SHIN B PARK W Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy J Microbiol 2017 55 11 837 849 10.1007/s12275-017-7288-4 SHIN B, PARK W.Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy[J].J Microbiol, 2017, 55(11):837-849.DOI:10.1007/s12275-017-7288-4.

ZHANG Y YAO Z ZHAN S Disease burden of intensive care unit-acquired pneumonia in China:a systematic review and meta-analysis Int J Infect Dis 2014 29 84 90 10.1016/j.ijid.2014.05.030 ZHANG Y, YAO Z, ZHAN S, et al .Disease burden of intensive care unit-acquired pneumonia in China:a systematic review and meta-analysis[J ] .Int J Infect Dis, 2014, 29:84-90.DOI:10.1016/j.ijid.2014.05.030.

MICHALOPOULOS A FALAGAS M E Treatment of Acinetobacter infections Exp Opin Pharmacother 2010 11 5 779 788 10.1517/14656561003596350 MICHALOPOULOS A, FALAGAS M E.Treatment of Acinetobacter infections[J].Exp Opin Pharmacother, 2010, 11(5):779-788.

JARURATANASIRIKUL S SUDSAI T Comparison of the pharmacodynamics of imipenem in patients with ventilator-associated pneumonia following administration by 2 or 0.5 h infusion J Antimicrob Chemother 2009 63 3 560 563 10.1093/jac/dkn543 JARURATANASIRIKUL S, SUDSAI T.Comparison of the pharmacodynamics of imipenem in patients with ventilator-associated pneumonia following administration by 2 or 0.5 h infusion[J].J Antimicrob Chemother, 2009, 63(3):560-563.DOI:10.1093/jac/dkn543.

PATEL J B COCKRILL Ⅲ F R ALDER J Performance standards for antimicrobial susceptibility testing 25th ed Wayne Clinical Laboratory Standards Institute 2015 56 57 PATEL J B, COCKRILL Ⅲ F R, ALDER J, et al .Performance standards for antimicrobial susceptibility testing[M ] .25th ed.Wayne:Clinical Laboratory Standards Institute, 2015:56-57.

杨馥嘉冯伟孙凤军我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析第三军医大学学报 2018 40 3 259 263 10.16016/j.1000-5404.201709112.YANGFJ, http://aammt.tmmu.edu.cn/Upload/rhtml/201709112.htm 杨馥嘉, 冯伟, 孙凤军.我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析[J].第三军医大学学报, 2018, 40(3):259-263.DOI:10.16016/j.1000-5404.201709112.

YANG F J FENG W SUN F J Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing J Third Mil Med Univ 2018 40 3 259 263 10.16016/j.1000-5404.201709112 http://aammt.tmmu.edu.cn/Upload/rhtml/201709112.htm YANG F J, FENG W, SUN F J.Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing[J ] .J Third Mil Med Univ, 2018, 40(3):259-263.DOI:10.16016/j.1000-5404.201709112.

SUN F OU Q WANG Q The resistance and transmission mechanism of Acinetobacter baumannii isolates in a tertiary care hospital, China J Chemother 2016 28 6 476 481 10.1080/1120009X.2016.1139335 SUN F, OU Q, WANG Q, et al .The resistance and transmission mechanism of Acinetobacter baumannii isolates in a tertiary care hospital, China[J ] .J Chemother, 2016, 28(6):476-481.DOI:10.1080/1120009X.2016.1139335.

KIM Y H LEE Y KIM S The role of periplasmic antioxidant enzymes (superoxide dismutase and thiol peroxidase) of the Shiga toxin-producing Escherichia coli O157:H7 in the formation of biofilms Proteomics 2006 6 23 6181 6193 10.1002/pmic.200600320 KIM Y H, LEE Y, KIM S, et al .The role of periplasmic antioxidant enzymes (superoxide dismutase and thiol peroxidase) of the Shiga toxin-producing Escherichia coli O157:H7 in the formation of biofilms[J ] .Proteomics, 2006, 6(23):6181-6193.DOI:10.1002/pmic.200600320.

XU Z G GAO Y HE J G Effects of azithromycin on pseudomonas aeruginosa isolates from catheter-associated urinary tract infection Exp Ther Med 2015 9 2 569 572 10.3892/etm.2014.2120 XU Z G, GAO Y, HE J G, et al .Effects of azithromycin on pseudomonas aeruginosa isolates from catheter-associated urinary tract infection[J ] .Exp Ther Med, 2015, 9(2):569-572.DOI:10.3892/etm.2014.2120.

ASADISHAD B HIDALGO G TUFENKJI N Pomegranate materials inhibit flagellin gene expression and flagellar-propelled motility of uropathogenic Escherichia coli strain CFT073 FEMS Microbiol Lett 2012 334 2 87 94 10.1111/j.1574-6968.2012.02622.x ASADISHAD B, HIDALGO G, TUFENKJI N.Pomegranate materials inhibit flagellin gene expression and flagellar-propelled motility of uropathogenic Escherichia coli strain CFT073[J].FEMS Microbiol Lett, 2012, 334(2):87-94.DOI:10.1111/j.1574-6968.2012.02622.x.

MURAKAMI K ONO T NOMA Y Explorative gene analysis of antibiotic tolerance-related genes in adherent and biofilm cells of Pseudomonas aeruginosa J Infect Chemother 2017 23 5 271 277 10.1016/j.jiac.2017.01.004 MURAKAMI K, ONO T, NOMA Y, et al .Explorative gene analysis of antibiotic tolerance-related genes in adherent and biofilm cells of Pseudomonas aeruginosa[J ] .J Infect Chemother, 2017, 23(5):271-277.DOI:10.1016/j.jiac.2017.01.004.

FURUKAWA S Studies on formation, control and application of biofilm formed by food related microorganisms Biosci Biotechnol Biochem 2015 79 7 1050 1056 10.1080/09168451.2015.1018126 FURUKAWA S.Studies on formation, control and application of biofilm formed by food related microorganisms[J].Biosci Biotechnol Biochem, 2015, 79(7):1050-1056.DOI:10.1080/09168451.2015.1018126.

KIM H A RYU S Y SEO I Biofilm formation and colistin susceptibility of acinetobacter baumannii isolated from korean nosocomial samples Microb Drug Resist 2015 21 4 452 457 10.1089/mdr.2014.0236 KIM H A, RYU S Y, SEO I, et al .Biofilm formati on and colistin susceptibility of acinetobacter baumannii isolated from korean nosocomial samples[J ] .Microb Drug Resist, 2015, 21(4):452-457.DOI:10.1089/mdr.2014.0236.

GREENE C WU J RICKARD A H Evaluation of the ability of Acinetobacter baumannii to form biofilms on six different biomedical relevant surfaces Lett Appl Microbiol 2016 63 4 233 239 10.1111/lam.12627 GREENE C, WU J, RICKARD A H, et al .Evaluation of the ability of Acinetobacter baumannii to form biofilms on six different biomedical relevant surfaces[J ] .Lett Appl Microbiol, 2016, 63(4):233-239.DOI:10.1111/lam.12627.

BAGGE N SCHUSTER M HENTZER M Pseudomonas aeruginosa biofilms exposed to imipenem exhibit changes in global gene expression and beta-lactamase and alginate production Antimicrob Agents Chemother 2004 48 4 1175 1187 10.1128/aac.48.4.1175-1187.2004 BAGGE N, SCHUSTER M, HENTZER M, et al .Pseudomonas aeruginosa biofilms exposed to imipenem exhibit changes in global gene expression and beta-lactamase and alginate production[J ] .Antimicrob Agents Chemother, 2004, 48(4):1175-1187.DOI:10.1128/aac.48.4.1175-1187.2004.

HARDING C M TRACY E N CARRUTHERS M D Acinetobacter baumannii strain M2 produces type Ⅵ pili which play a role in natural transformation and twitching motility but not surface-associated motility MBio 2013 4 4 e00360 13 10.1128/mBio.00360-13 HARDING C M, TRACY E N, CARRUTHERS M D, et al . Acinetobacter baumannii strain M2 produces type Ⅵ pili which play a role in natural transformation and twitching motility but not surface-associated motility[J ] .MBio, 2013, 4(4):e00360-13.DOI:10.1128/mBio.00360-13.

HACKER J OTT M HOF H Effects of low, subinhibitory concentrations of antibiotics on expression of a virulence gene cluster of pathogenic Escherichia coli by using a wild-type gene fusion Int J Antimicrob Agents 1993 2 4 263 270 10.1016/0924-8579(93)90060-i HACKER J, OTT M, HOF H.Effects of low, subinhibitory concentrations of antibiotics on expression of a virulence gene cluster of pathogenic Escherichia coli by using a wild-type gene fusion[J].Int J Antimicrob Agents, 1993, 2(4):263-270.DOI:10.1016/0924-8579(93)90060-i.

BRAGA P C SASSO M D SALA M T Sub-MIC concentrations of cefodizime interfere with various factors affecting bacterial virulence J Antimicrob Chemother 2000 45 1 15 25 10.1093/jac/45.1.15 BRAGA P C, SASSO M D, SALA M T.Sub-MIC concentrations of cefodizime interfere with various factors affecting bacterial virulence[J].J Antimicrob Chemother, 2000, 45(1):15-25.DOI:10.1093/jac/45.1.15.

FLORES-KIM J DARWIN A J Regulation of bacterial virulence gene expression by cell envelope stress responses Virulence 2014 5 8 835 851 10.4161/21505594.2014.965580 FLORES-KIM J, DARWIN A J.Regulation of bacterial virulence gene expression by cell envelope stress responses[J].Virulence, 2014, 5(8):835-851.DOI:10.4161/21505594.2014.965580.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

亚抑菌浓度头孢他啶对临床尿管分离大肠埃希菌生物膜的作用

基于CT影像的3D ResNet18模型在肺结核耐药性识别中的预测性能研究

大黄素抑制MRSA生物膜活性及其作用机制研究

长沙市122例输入性恶性疟原虫pfpm2基因拷贝数变异的分析

抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究