碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞生物学行为的影响

李翔; 陈瑶; 周鑫; 樊东力; 张一鸣; 燕荣帅

doi:10.16016/j.1000-5404.201807117

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碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞生物学行为的影响

基础医学 | 更新时间：2026-01-09

- 碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞生物学行为的影响
- Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells
- 第三军医大学学报 2018年40卷第23期页码：2162-2167, 2189
- 作者机构：
  
  400037 重庆, 陆军军医大学(第三军医大学)第二附属医院整形美容外科
- 作者简介：
  
  [ 燕荣帅, E-mail: ericyan_lead@163.com YAN Rongshuai, E-mail:ericyan_lead@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81571918)
- DOI：10.16016/j.1000-5404.201807117
  中图分类号： R318.08;R329.21;R329.28
- 收稿：2018-07-10，
  
  修回：2018-08-06，
  
  纸质出版：2018-12-15
- 稿件说明：
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李翔, 陈瑶, 周鑫, 等. 碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞生物学行为的影响[J]. 第三军医大学学报, 2018,40(23):2162-2167, 2189.

Xiang LI, Yao CHEN, Xin ZHOU, et al. Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(23): 2162-2167, 2189.
李翔, 陈瑶, 周鑫, 等. 碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞生物学行为的影响[J]. 第三军医大学学报, 2018,40(23):2162-2167, 2189. DOI： 10.16016/j.1000-5404.201807117.

Xiang LI, Yao CHEN, Xin ZHOU, et al. Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(23): 2162-2167, 2189. DOI： 10.16016/j.1000-5404.201807117.

摘要

目的

探讨碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞黏附、增殖和分化的影响。

方法

以浓度为1×10

ions/cm

在10KeV条件下向普通硅橡胶注入碳离子、银离子和氮离子

得到碳硅胶(C-SR)、银硅胶(Ag-SR)和氮硅胶(N-SR)3组改性的硅橡胶材料并作为实验组

普通硅橡胶(SR)作为对照组

从130g成年SD大鼠骨髓取2~5代骨髓间充质干细胞进行生物相容性实验。通过CCK-8观察细胞增殖

并在普通光镜下比较黏附细胞的数量和形态

F-actin免疫荧光染色观察细胞骨架形态的变化、Western blot检测成骨及成脂分化相关标志物的表达。

结果

成功将离子注入硅橡胶中

得到碳硅胶(C-SR)、银硅胶(Ag-SR)和氮硅胶(N-SR)3种改性的硅橡胶材料。大鼠骨髓间充质干细胞能较好地粘附在改性后的硅橡胶材料上

并且N-SR上细胞增殖较明显

与硅橡胶上的细胞增殖差异有统计学意义(

0.05)。通过F-actin免疫荧光染色

不同材料上的细胞骨架形态发生变化

Western blot检测结果显示N-SR有促进成骨分化的趋势。

结论

碳、银和氮离子注入硅橡胶提高了材料表面的生物相容性

其中N-SR能促进细胞的粘附与增殖

并对干细胞的分化产生影响。

Abstract

Objective

To investigate the effects of carbon

silver and nitrogen ions-implanted silicone rubber on the adhesion

proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMMSCs).

Methods

The surface of the virgin silicone rubber was implanted with carbon ions

silver ions and nitrogen ions under the condition of 10 KeV at 1×10

ions/cm

to obtain carbon silica gel (C-SR)

silver silica gel (Ag-SR) and nitrogen silica gel (N-SR). And these gels served as the experimental groups

while the virgin silicone rubber as control group. The BMMSCs were isolated from the 130 g adult SD rats

then the P2 to P5 generation cells were cultured on all above materials. Then CCK-8 assay was used to observe the proliferation of cells

and the number and morphology of adherent cells were observed under a photomicrographe. F-actin immunofluorescence staining was used to observe the change of cytoskeleton

and Western blot assay was used to detect the differentiation of stem cells.

Results

The carbon

silver and nitrogen ions were successfully implanted into virgin silicone rubber

and the obtained 3 kinds of modified silicone rubber materials were named as C-SR

Ag-SR and N-SR

respectively. When the rat BMMSCs were cultured on the ion-implanted silicone rubber materials

the cells better adhered to the materials

especially on N-SR. Meanwhile

the cells growing on the N-SR material showed better proliferation

with significant difference with those growing on virgin silicone rubber (

0.05). The results of F-actin immunofluorescence staining indicated that the cytoskeleton morphology on ion-implanted silicone rubber materials had changed

and Western blot results identified that the BMMSCs had a tendency towards osteogenic differentiation.

Conclusion

The carbon

silver and nitrogen ions-implanted silicone rubber materials improve the biocompatibility of the surface of the silicone rubber. N-SR material can promote the adhesion and proliferation and influence the differentiation of the stem cells.

关键词

Keywords

references

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