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2018年成都周边部队感染55型腺病毒分子流行病学溯源
Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area
- 第三军医大学学报 2018年40卷第24期 页码：2211-2216
- 作者机构：
1.610021 成都, 西部战区疾病预防控制中心
2.610083 成都, 西部战区总医院检验科
- 作者简介：
[范泉水, E-mail：13888254888@126.com FAN Quanshui, E-mail: 13888254888@126.com
[胡小兵, E-mail：18981715872@189.cn HU Xiaobing, E-mail: 18981715872@189.cn
- 基金信息：中国博士后科学基金(2018T111157);中国博士后科学基金(2017M623427);中国博士后科学基金(2017M613427);中国博士后科学基金(2017T100820)
DOI：10.16016/j.1000-5404.201807082
中图分类号： R181.81; R372; R511.8收稿：2018-07-11，
修回：2018-09-25，
纸质出版：2018-12-30
- 稿件说明：
移动端阅览
王文博, 古良琪, 刘媛, 等. 2018年成都周边部队感染55型腺病毒分子流行病学溯源[J]. 第三军医大学学报, 2018,40(24):2211-2216.
Wenbo WANG, Liangqi GU, Yuan LIU, et al. Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area[J]. Journal of Third Military Medical University, 2018, 40(24): 2211-2216.
王文博, 古良琪, 刘媛, 等. 2018年成都周边部队感染55型腺病毒分子流行病学溯源[J]. 第三军医大学学报, 2018,40(24):2211-2216. DOI： 10.16016/j.1000-5404.201807082.
Wenbo WANG, Liangqi GU, Yuan LIU, et al. Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area[J]. Journal of Third Military Medical University, 2018, 40(24): 2211-2216. DOI： 10.16016/j.1000-5404.201807082.
摘要
目的
2
对成都地区驻地部队暴发55型腺病毒进行病原分离、全基因序列及和遗传进化特点等分子流行病学分析，为揭示55型腺病毒的进化起源及制订疾病防控策略提供参考数据。
方法
2
采集55型腺病毒感染者咽拭子标本，采用人喉癌上皮细胞HEp-2分离病毒株。利用分片段PCR和测序获取毒株的全基因序列，检索并下载GenBank数据库中55型腺病毒全基因序列，采用生物信息学分析软件-MEGA6.06进行序列比对和系统发育进化树构建。
结果
2
从成都周边驻地部队3起感染疫情中分离出55型腺病毒5株，分别为24-1株、24-2株、1804001株、1806003株、DYN株。采用12片段分段PCR和测序获得了5株病毒全基因序列，GenBank登录号分别为MH256653~MH256657，5株病毒之间序列差异较小，仅有16处碱基变异。与我国报道的第一株55型腺病毒QS-DLL株和重庆株(CQ-2903)序列相比，有30处碱基变异。根据系统发育进化树分析结果，新分离的5株HAdV-55毒株均与QS-DLL株相近，与我们之前分离的西藏株(LS89/Tibet/2016)、云南株(KM03/YN/2016)等进化距离相对较远。
结论
2
本研究分析发现2018年成都周边驻地部队流行的55型腺病毒为QS-DLL类似株或重庆分离株CQ-2903株的突变株。
Abstract
Objective
2
To conduct a molecular epidemiological analysis of 3 human adenovirus type 55 (HAdV-55) outbreaks in the year 2018 in military forces in Chengdu area and obtain epidemiological evidence to identify the evolutionary origin of HAdV-55 for formulating disease prevention and control strategies.
Methods
2
Throat swab specimens from HAdV-55-infected individuals were collected for virus isolation
via
HEp-2 cells. The full-length DNA sequences of the isolated HAdV-55 strains were obtained with segmented PCR and sequencing. The whole genome sequences of HAdV-55 retrieved from the GenBank database were used for sequence alignment and phylogenetic tree construction using bioinformatics analysis software MEGA6.06.
Results
2
Five strains of HAdV-55
namely 24-1 strain
24-2 strain
1804001 strain
1806003 strain
and DYN strain
were isolated from 3 outbreaks in the military forces stationed around Chengdu City. The whole genome sequences of these 5 HAdV-55 strains (GenBank accession numbers MH256653
MH256654
MH256655
MH256656
and MH256657
respectively) obtained by 12-segment fragmentation PCR sequencing showed only minor variations with mutations of 16 bases. Compared with the sequence of the first isolated HAdV-55 strain (QS-DLL) in China
these strains showed mutations at 30 bases. Phylogenetic tree analysis showed that the 5 newly isolated HAdV-55 strains were similar to the QS-DLL strain and the Chongqing strain (CQ-2903)
and their evolutionary distances were greater from the previously isolated Tibet strain (LS89/Tibet/2016) and Yunnan strain (KM03/YN/2016).
Conclusion
2
The HAdV-55 strains prevalent in the 2018 outbreaks in the military forces around Chengdu are QS-DLL-like strains or mutant strains of CQ-2903.
关键词
Keywords
references
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