丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力

吴悠; 殷开宇; 李远; 彭明清

doi:10.16016/j.1000-5404.201806188

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丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力

基础医学 | 更新时间：2026-01-09

- 丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力
- Propofol inhibits invasion and migration of breast cancer cells via down-regulating transforming growth factor-β1
- 第三军医大学学报 2018年40卷第24期页码：2236-2242
- 作者机构：
  
  1.402160 重庆，重庆医科大学附属永川医院:麻醉科
  2.402160 重庆，重庆医科大学附属永川医院:中心实验室
- 作者简介：
  
  [彭明清, E-mail：315747391@qq.com PENG Mingqing, E-mail: 315747391@qq.com
- 基金信息：
  
  重庆医科大学附属永川医院研究生创新基金(YJSCX201707)
- DOI：10.16016/j.1000-5404.201806188
  中图分类号： R73-37;R737.9;R971.2
- 收稿：2018-06-28，
  
  修回：2018-09-29，
  
  纸质出版：2018-12-30
- 稿件说明：
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吴悠, 殷开宇, 李远, 等. 丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力[J]. 第三军医大学学报, 2018,40(24):2236-2242.

You WU, Kaiyu YIN, Yuan LI, et al. Propofol inhibits invasion and migration of breast cancer cells via down-regulating transforming growth factor-β1[J]. Journal of Third Military Medical University, 2018, 40(24): 2236-2242.
吴悠, 殷开宇, 李远, 等. 丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力[J]. 第三军医大学学报, 2018,40(24):2236-2242. DOI： 10.16016/j.1000-5404.201806188.

You WU, Kaiyu YIN, Yuan LI, et al. Propofol inhibits invasion and migration of breast cancer cells via down-regulating transforming growth factor-β1[J]. Journal of Third Military Medical University, 2018, 40(24): 2236-2242. DOI： 10.16016/j.1000-5404.201806188.

摘要

目的

探究丙泊酚通过TGF-β1对乳腺癌细胞迁移和侵袭能力的影响。

方法

将体外培养的乳腺癌MCF-7细胞分为空白对照组、DMSO处理组、25 μg/mL丙泊酚处理组、10 ng/mL TGF-β1和25 μg/mL丙泊酚共同处理组。采用蛋白免疫印记法检测转化生长因子β1、E-钙黏蛋白表达水平及基质金属蛋白酶MMP-9蛋白表达水平；Transwell迁移、侵袭实验和体外血管拟态形成试验检测细胞迁移、侵袭和血管生成能力的变化。

结果

① 丙泊酚处理组与空白对照组相比TGF-β1和MMP-9蛋白表达水平明显降低(

＜0.05，

＜0.01)，E-cadherin蛋白表达水平增加(

＜0.05)，且Transwell迁移实验穿膜细胞数(84.7±4.5)，Transwell侵袭实验穿膜细胞数(130.7±4.3)和新生血管管腔形成数量(53.7±5.6)较空白对照组(163.3±8.3)，(190.7±8.4)，(108.0±7.6)显著减少(

＜0.01)，说明丙泊酚能抑制乳腺癌MCF-7细胞迁移、侵袭和新生血管形成；②TGF-β1和丙泊酚共同处理组，与丙泊酚处理组相比TGF-β1和MMP-9蛋白表达水平升高(

＜0.05，

＜0.01)，E-cadherin蛋白表达水平降低(

＜0.001)，穿膜细胞数(180.3±9.6)，(172.0±10.3)和新生血管管腔形成数量(107.3±8.6)较丙泊酚处理组(100.7±4.9)，(57.3±5.7)，(50.7±5.8)显著增加(

＜0.05)，说明TGF-β1能逆转丙泊酚对MCF-7的抑制作用。

结论

丙泊酚通过下调TGF-β1抑制乳腺癌细胞MCF-7细胞迁移、侵袭能力。

Abstract

Objective

To investigate the inhibitory effect of propofol on the migration and invasion of breast cancer cells and the role of transforming growth factor-β1 (TGF-β1) in mediating this effect.

Methods

Cultured breast cancer MCF-7 cells were treated with DMSO

25 μg/mL propofol

or 25 μg/mL propofol plus 10 ng/mL TGF-β1. Western blotting was used to detect the expression of TGF-β1

MMP-9 and E-cadherin in the cells

and Transwell migration assay

Matrigel invasion assay and vascular mimicry assay were performed to assess the changes in the cell migration

invasion and angiogenesis in response to the treatments.

Results

Compared with the blank control cells

the cells with propofol treatment alone showed significantly decreased expression levels of TGF-β1 (

0.05) and MMP-9 (

0.01) and increased expression level of E-cadherin (

0.05). Compared with the control cells

propofol treatment significantly reduced the number of migrating cells in Transwell migration assay (163.3±8.4

84.7±4.5)

the number of invasive cells in Matrigel invasion assay (190.4±8.4

130.7±4.3)

and the number of neovascular lumens (108.0±7.6

53.7±5.6) (

0.01). Compared with the propofol-treated cells

the cells treated with both TGF-β1 and propofol exhibited

significant up-regulation of the expression of TGF-β1 (

0.05) and MMP-9 (

0.01) and down-regulation of E-cadherin expression (

0.001)

with obviously increased numbers of migrating cells (100.7±4.9

180.3±9.6)

invasive cells (57.3±5.7

172.0±10.3)

and neovascular lumens (50.7±5.8

107.3±8.6) (

0.05).

Conclusion

Propofol can inhibit the migration and invasion of breast cancer cells

in vitro

by down-regulating TGF-β1.

关键词

Keywords

references

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