Linc00238在乙型肝炎病毒复制中的调控作用

黄琴; 张祯祯; 刘泉波

doi:10.16016/j.1000-5404.201805100

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Linc00238在乙型肝炎病毒复制中的调控作用

基础医学 | 更新时间：2026-01-09

- Linc00238在乙型肝炎病毒复制中的调控作用
- Effect of long intergenic non-coding RNA 238 on HBV replication and expression in vitro
- 第三军医大学学报 2018年40卷第21期页码：1942-1947
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金(81501736);高等学校博士学科点专项科研基金(20135503120008)
- DOI：10.16016/j.1000-5404.201805100
  中图分类号：
- 收稿：2018-05-13，
  
  纸质出版：2018-11-15
- 稿件说明：
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黄琴, 张祯祯, 刘泉波. Linc00238在乙型肝炎病毒复制中的调控作用[J]. 第三军医大学学报, 2018,40(21):1942-1947.

Qin HUANG, Zhenzhen ZHANG, Quanbo LIU. Effect of long intergenic non-coding RNA 238 on HBV replication and expression in vitro[J]. Journal of Third Military Medical University, 2018, 40(21): 1942-1947.
黄琴, 张祯祯, 刘泉波. Linc00238在乙型肝炎病毒复制中的调控作用[J]. 第三军医大学学报, 2018,40(21):1942-1947. DOI： 10.16016/j.1000-5404.201805100.

Qin HUANG, Zhenzhen ZHANG, Quanbo LIU. Effect of long intergenic non-coding RNA 238 on HBV replication and expression in vitro[J]. Journal of Third Military Medical University, 2018, 40(21): 1942-1947. DOI： 10.16016/j.1000-5404.201805100.

摘要

Abstract

Objective

To determine the regulating role of long intergenic non-coding RNA 00238 (Linc00238) in the replication of hepatitis B virus (HBV) and explore the potential mechanism.

Methods

HBV positive and HBV negative liver tissues were proceeded to LincRNA array

and then the interested Linc00238 was confirmed further by real-time PCR in HBV expression cell line HepAD38

HepG2 cells as well as HBV transient transfection cell line HepG2.2.15. After overexpression and RNA interference techniques

the effect of Linc00238 on the replication

transcription and expression of HBV was determined by gain-of-function and loss-of-function study in HepAD38 cells. Dual-luciferase reporter assay was used to detect the effect of Linc00238 on promoter activity of HBV.

Results

LincRNA array indicated there were 13 LincRNAs having changed expression levels in HBV positive liver tissues

with 2 up-regulated and 11 down-regulated. Real-time PCR further showed the expression level of Linc00238 was significantly down-regulated in HBV positive liver tissues

HBV expression cell line and HBV transient expression HepG2.2.15 cells (

0.05)

which indicating that HBV infection inhibited the expression of Linc00238. Overexpression of Linc00238 suppressed HBV replication and protein levels significantly in HepG2 (

0.05)

and while sh00238 inte

rference restored the HBV replication and protein levels (

0.05). The reporter luciferase reporter system showed no changes were found after the co-transfection of pHBs-luc

pHBx-luc

pHBc-luc and pCDNA-linc00238

indicating that Linc00238 has no effect on HBV promoter activity (

0.05).

Conclusion

Linc00238 exerts inhibitory effect on HBV protein and DNA expression

but this effect is not associated with HBV promoter activities.

关键词

Keywords

references

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