miR-1322在低氧诱导动脉平滑肌细胞增殖中的作用及其机制

周咏梅; 王斌; 高瞻; 张明周; 李瑾; 魏征华; 游节根; 贺斌峰

doi:10.16016/j.1000-5404.201805089

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miR-1322在低氧诱导动脉平滑肌细胞增殖中的作用及其机制

基础医学 | 更新时间：2026-01-09

- miR-1322在低氧诱导动脉平滑肌细胞增殖中的作用及其机制
- Role and mechanism of miR-1322 in proliferation of artery smooth muscle cells induced by hypoxia in vitro
- 第三军医大学学报 2018年40卷第21期页码：1948-1955
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部重点研发课题(2016YFC1304503);国家自然科学基金面上项目(81370168);国家自然科学基金面上项目(81670047)
- DOI：10.16016/j.1000-5404.201805089
  中图分类号：
- 收稿：2018-05-11，
  
  纸质出版：2018-11-15
- 稿件说明：
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周咏梅, 王斌, 高瞻, 等. miR-1322在低氧诱导动脉平滑肌细胞增殖中的作用及其机制[J]. 第三军医大学学报, 2018,40(21):1948-1955.

Yongmei ZHOU, Bin WANG, Zhan GAO, et al. Role and mechanism of miR-1322 in proliferation of artery smooth muscle cells induced by hypoxia in vitro[J]. Journal of Third Military Medical University, 2018, 40(21): 1948-1955.
周咏梅, 王斌, 高瞻, 等. miR-1322在低氧诱导动脉平滑肌细胞增殖中的作用及其机制[J]. 第三军医大学学报, 2018,40(21):1948-1955. DOI： 10.16016/j.1000-5404.201805089.

Yongmei ZHOU, Bin WANG, Zhan GAO, et al. Role and mechanism of miR-1322 in proliferation of artery smooth muscle cells induced by hypoxia in vitro[J]. Journal of Third Military Medical University, 2018, 40(21): 1948-1955. DOI： 10.16016/j.1000-5404.201805089.

摘要

Abstract

Objective

To investigate the regulating effect of miR-1322 on the proliferation of human pulmonary artery smooth muscle cells (HPASMCs) induced by hypoxia and study the possible mechanism.

Methods

After HPASMCs were exposed to hypoxia for 0

24 and 48 h respectively

the expression level of miR-1322 was evaluated using RT-qPCR

the protein levels of BMPR2

p-smad1/5/9 and p21 were detected by Western blotting

and cell proliferation was measured using CCK-8 assay. After HPASMCs were transfected respectively with miR-1322 inhibitor and negative control for 24 h

the cells were exposed to hypoxia for further 48 h. The proliferation of HPASMCs was detected by CCK-8 assay at 12

24 and 48 h. Luciferase reporter gene assay was used to evaluate whether miR-1322 binding BMPR2 3'untranslated regions (UTR). The protein levels of p-smad1/5/9 and p21 were evaluated using Western blotting. HPASMCs were transfected with pcDNA3.1-BMPR2 plasmid for 24 h

and then further exposed to 48 h. The cell proliferation and protein expression of p-smad1/5/9 and p21 were also evaluated.

Results

The level of miR-1322 was significantly higher in the HPASMCs after hypoxia exposure for 12

24 and 4 8h than untreated cells (

0.05). miR-1322 inhibitor suppressed the level of miR-1322 than the NC group of cells (

0.05). The cell proliferation was significantly decreased in the cells of miR-1322 inhibitor/hypoxia group after 24 and 48 h than the cells only exposed to hypoxia alone (

0.05). Luciferase assay showed that BMPR2 was target of miR-1322. Furthermore

transfection of miR-1322 mimic decreased the expression of BMPR2 protein than the NC group (

0.05)

while miR-1322 inhibitor treatment improved the protein level than the NC group (

0.05). The expression levels of BMPR2 and p21 were significant lower at 6

12 and 24 h after hypoxia than the NC group at same time points (

0.05)

whereas

the expression of p-smad1/5/9 was obvious higher (

0.05). The protein levels of p-smad1/5/9 and p21 were higher in the BMPR2 overexpression/hypoxia group than the vector/hypoxia group (

0.05). CCK-8 assay showed that the proliferation HAPSMCs was lower in BMPR2 overexpression group than in vector group (

0.05).

Conclusion

Hypoxia improves the expression of miR-1322 in HPASMCs. Up-regulation of miR-1322 promotes HPASMCs proliferation through inhibiting BMPR2 to mediate p-smad1/5/9-p21 signal pathway.

关键词

Keywords

references

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