纳米材料富勒醇对铅中毒大鼠肝肾功能损伤及血液学异常表现的修复作用

查盈盈; 葛进; 郑超; 余小文

doi:10.16016/j.1000-5404.201805079

您当前的位置：

首页 >

文章列表页 >

纳米材料富勒醇对铅中毒大鼠肝肾功能损伤及血液学异常表现的修复作用

基础医学 | 更新时间：2026-01-09

- 纳米材料富勒醇对铅中毒大鼠肝肾功能损伤及血液学异常表现的修复作用
- Nanomaterial fullerenol attenuates liver and kidney impairments and hematological abnormalities in lead-poisoned rats
- 第三军医大学学报 2018年40卷第21期页码：1962-1966,1977
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金(81601158);安徽省自然科学基金青年科学基金(1508085SQC203)
- DOI：10.16016/j.1000-5404.201805079
  中图分类号：
- 收稿：2017-11-15，
  
  纸质出版：2018-11-15
- 稿件说明：
移动端阅览
查盈盈, 葛进, 郑超, 等. 纳米材料富勒醇对铅中毒大鼠肝肾功能损伤及血液学异常表现的修复作用[J]. 第三军医大学学报, 2018,40(21):1962-1966,1977.

Yingying ZHA, Jin GE, Chao ZHENG, et al. Nanomaterial fullerenol attenuates liver and kidney impairments and hematological abnormalities in lead-poisoned rats[J]. Journal of Third Military Medical University, 2018, 40(21): 1962-1966,1977.
查盈盈, 葛进, 郑超, 等. 纳米材料富勒醇对铅中毒大鼠肝肾功能损伤及血液学异常表现的修复作用[J]. 第三军医大学学报, 2018,40(21):1962-1966,1977. DOI： 10.16016/j.1000-5404.201805079.

Yingying ZHA, Jin GE, Chao ZHENG, et al. Nanomaterial fullerenol attenuates liver and kidney impairments and hematological abnormalities in lead-poisoned rats[J]. Journal of Third Military Medical University, 2018, 40(21): 1962-1966,1977. DOI： 10.16016/j.1000-5404.201805079.

摘要

Abstract

Objective

To investigate the repair effects of nanomaterial fullerenol on the liver and kidney impairments and hematological abnormalities in lead-poisoned rats and explore its possible mechanism.

Methods

Newborn Wistar rats (5±1 g

regardless of sex) were randomly divided into normal control group (

=10

normal feeding)

lead-poisoning model group (

=10

0.2% of lead acetate in drinking water) and fullerenol intervention group (

=10

0.2% of lead acetate in drinking water

intraperitoneal injection of 5 mg/kg fullerenol once every day for 10 d from the age of 3 weeks). When the rats were at 5 weeks old

they were sacrificed

and HE staining was used to observe the pathological changes in the liver and kidney tissues. And the hematological indexes were analyzed using routine blood test. Total nitric oxide (NO) concentration detection kit and Western blotting were employed to measure the concentration of NO and the expression of inducible nitric oxide synthase (iNOS)

respectively.

Results

Fullerenol greatly changed the morphological abnormalities of liver and kidney

including hepatocyte swelling

nuclear conde

nsation and glomerular atrophy. Meanwhile

fullerenol significantly increased the hematological indexes associated with anemia (all

0.05)

such as red blood cell (RBC)

hemoglobin (HGB)

mean corpuscle volume (MCV) and mean corpuscle concentration (MCHC). What's more

compared with the lead-poisoning model group (0.58±0.06)

the expression of iNOS in the brain tissue was significantly reduced in the fullerenol intervention group (0.39±0.05

0.05).

Conclusion

By regulating NO-NOS system

fullerenol repairs the damages caused by lead poisoning

including the morphological abnormalities of the liver and kidneys as well as the important hematological indexes related to anemia.

关键词

Keywords

references

BOGDANOVIC G DJORDJEVIC A Carbon nanomaterials:Biologically active fullerene derivatives Srp Arh Celok Lek 2016 144 3/4 222 231 http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0210088655/ BOGDANOVIC G, DJORDJEVIC A.Carbon nanomaterials:Biologically active fullerene derivatives[J].Srp Arh Celok Lek, 2016, 144(3/4):222-231.

SHI J ZHANG H WANG L PEI-derivatized fullerene drug delivery using folate as a homing device targeting to tumor Biomaterials 2013 34 1 251 261 10.1016/j.biomaterials.2012.09.039 SHI J, ZHANG H, WANG L, et al .PEI-derivatized fullerene drug delivery using folate as a homing device targeting to tumor[J ] .Biomaterials, 2013, 34(1):251-261.DOI:10.1016/j.biomaterials.2012.09.039.

FAN J FANG G ZENG F Water-dispersible fullerene aggregates as a targeted anticancer prodrug with both chemo-and photodynamic therapeutic actions Small 2013 9 4 613 621 10.1002/smll.201201456 FAN J, FANG G, ZENG F, et al .Water-dispersible fullerene aggregates as a targeted anticancer prodrug with both chemo-and photodynamic therapeutic actions[J ] .Small, 2013, 9(4):613-621.DOI:10.1002/smll.201201456.

GANAPATHY V THANGARAJU M PRASAD P D Nutrient transporters in cancer:relevance to Warburg hypothesis and beyond Pharmacol Ther 2009 121 1 29 40 10.1016/j.pharmthera.2008.09.005 GANAPATHY V, THANGARAJU M, PRASAD P D.Nutrient transporters in cancer:relevance to Warburg hypothesis and beyond[J].Pharmacol Ther, 2009, 121(1):29-40.DOI:10.1016/j.pharmthera.2008.09.005.

SHOJI M TAKAHASHI E HATAKEYAMA D Anti-influenza activity of C60 fullerene derivatives PloS ONE 2013 8 6 e66337 10.1371/journal.pone.0066337 SHOJI M, TAKAHASHI E, HATAKEYAMA D, et al .Anti-influenza activity of C60 fullerene derivatives[J ] .PloS ONE, 2013, 8(6):e66337.DOI:10.1371/journal.pone.0066337.

ISAKOVIC A MARKOVIC Z TODOROVIC-MARKOVIC B Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene Toxicol Sci 2006 91 1 173 183 10.1093/toxsci/kfj127 ISAKOVIC A, MARKOVIC Z, TODOROVIC-MARKOVIC B, et al .Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene[J ] .Toxicol Sci, 2006, 91(1):173-183.DOI:10.1093/toxsci/kfj127.

DUGAN L L GABRIELSEN J K YU S P Buckminsterfullerenol free radical scavengers reduce excitotoxic and apoptotic death of cultured cortical neurons Neurobiol Dis 1996 3 2 129 135 10.1006/nbdi.1996.0013 DUGAN L L, GABRIELSEN J K, YU S P, et al .Buckminsterfullerenol free radical scavengers reduce excitotoxic and apoptotic death of cultured cortical neurons[J ] .Neurobiol Dis, 1996, 3(2):129-135.DOI:10.1006/nbdi.1996.0013.

JIN H CHEN W Q TANG X W Polyhydroxylated C (60), fullerenols, as glutamate receptor antagonists and neuroprotective agents J Neurosci Res 2000 62 4 600 607 10.1002/1097-4547(20001115)62:4<600::AID-JNR15>3.0.CO;2-F JIN H, CHEN W Q, TANG X W, et al .Polyhydroxylated C (60), fullerenols, as glutamate receptor antagonists and neuroprotective agents[J ] .J Neurosci Res, 2000, 62(4):600-607.DOI:10.1002/1097-4547(20001115)62:4 < 600::AID-JNR15 > 3.0.CO; 2-F.

WHITE L D CORY-SLECHTA D A GILBERT M E New and evolving concepts in the neurotoxicology of lead Toxicol Appl Pharm 2007 225 1 1 27 10.1016/j.taap.2007.08.001 WHITE L D, CORY-SLECHTA D A, GILBERT M E, et al .New and evolving concepts in the neurotoxicology of lead[J ] .Toxicol Appl Pharm, 2007, 225(1):1-27.DOI:10.1016/j.taap.2007.08.001.

NIU Y YU W FANG S Lead poisoning influences TCR-related gene expression patterns in peripheral blood T-lymphocytes of exposed workers J Immunotoxicol 2015 12 1 92 97 10.3109/1547691X.2014.899412 NIU Y, YU W, FANG S, et al .Lead poisoning influences TCR-related gene expression patterns in peripheral blood T-lymphocytes of exposed workers[J ] .J Immunotoxicol, 2015, 12(1):92-97.DOI:10.3109/1547691X.2014.899412.

NEEDLEMAN H Lead poisoning Annu Rev Med 2004 55 209 222 10.1146/annurev.med.55.091902.103653 NEEDLEMAN H.Lead poisoning[J].Annu Rev Med, 2004, 55:209-222.DOI:10.1146/annurev.med.55.091902.103653.

XU J YAN H C YANG B Effects of lead exposure on hippocampal metabotropic glutamate receptor subtype 3 and 7 in developmental rats J Negat Results Biomed 2009 8 5 10.1186/1477-5751-8-5 XU J, YAN H C, YANG B, et al .Effects of lead exposure on hippocampal metabotropic glutamate receptor subtype 3 and 7 in developmental rats[J ] .J Negat Results Biomed, 2009, 8:5.DOI:10.1186/1477-5751-8-5.

PARK S K O'NEILL M S VOKONAS P S Air pollution and heart rate variability:effect modification by chronic lead exposure Epidemiology 2008 19 1 111 120 10.1097/EDE.0b013e31815c408a PARK S K, O'NEILL M S, VOKONAS P S, et al .Air pollution and heart rate variability:effect modification by chronic lead exposure[J ] .Epidemiology, 2008, 19(1):111-120.DOI:10.1097/EDE.0b013e31815c408a.

LEE C M TERRIZZI A R BOZZINI C Chronic lead poisoning magnifies bone detrimental effects in an ovariectomized rat model of postmenopausal osteoporosis Exp Toxicol Pathol 2016 68 1 47 53 10.1016/j.etp.2015.09.007 LEE C M, TERRIZZI A R, BOZZINI C, et al .Chronic lead poisoning magnifies bone detrimental effects in an ovariectomized rat model of postmenopausal osteoporosis[J ] .Exp Toxicol Pathol, 2016, 68(1):47-53.DOI:10.1016/j.etp.2015.09.007.

CLEVELAND L M MINTER M L COBB K A Lead hazards for pregnant women and children:part 1:immigrants and the poor shoulder most of the burden of lead exposure in this country.Part 1 of a two-part article details how exposure happens, whom it affects, and the harm it can do Am J Nurs 2008 108 10 40 49 10.1097/01.NAJ.0000337736.76730.66 CLEVELAND L M, MINTER M L, COBB K A, et al .Lead hazards for pregnant women and children:part 1:immigrants and the poor shoulder most of the burden of lead exposure in this country.Part 1 of a two-part article details how exposure happens, whom it affects, and the harm it can do[J ] .Am J Nurs, 2008, 108(10):40-49;quiz 50.DOI:10.1097/01.NAJ.0000337736.76730.66.

ZHA Y Y YANG B TANG M L Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability Int J Nanomed 2012 7 3099 3109 10.2147/IJN.S30934 ZHA Y Y, YANG B, TANG M L, et al .Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability[J ] .Int J Nanomed, 2012, 7:3099-3109.DOI:10.2147/IJN.S30934.

WANG X X ZHA Y Y YANG B Suppression of synaptic p lasticity by fullerenol in rat hippocampus in vitro Int J Nanomed 2016 11 4947 4955 10.2147/IJN WANG X X, ZHA Y Y, YANG B, et al .Suppression of synaptic plasticity by fullerenol in rat hippocampus in vitro [J ] .Int J Nanomed, 2016, 11:4947-4955.

WEI T CHEN C ZHAO B EPC-K1 attenuates peroxynitrite-induced apoptosis in cerebellar granule cells Biochem Mol Biol Int 1998 46 1 89 97 10.1080/15216549800203592 WEI T, CHEN C, ZHAO B, et al .EPC-K1 attenuates peroxynitrite-induced apoptosis in cerebellar granule cells[J ] .Biochem Mol Biol Int, 1998, 46(1):89-97.DOI:10.1080/15216549800203592.

COSTA E D REZENDE B A CORTES S F Neuronal nitric oxide synthase in vascular physiology and diseases Front Physiol 2016 7 206 10.3389/fphys.2016.00206 COSTA E D, REZENDE B A, CORTES S F, et al .Neuronal nitric oxide synthase in vascular physiology and diseases[J ] .Front Physiol, 2016, 7:206.DOI:10.3389/fphys.2016.00206.

EL-SOKKARY G H ABDEL-RAHMAN G H KAMEL E S Melatonin protects against lead-induced hepatic and renal toxicity in male rats Toxicology 2005 213 1/2 25 33 10.1016/j.tox.2005.05.003 EL-SOKKARY G H, ABDEL-RAHMAN G H, KAMEL E S.Melatonin protects against lead-induced hepatic and renal toxicity in male rats[J].Toxicology, 2005, 213(1/2):25-33.DOI:10.1016/j.tox.2005.05.003.

TURGUT S POLAT A INAN M Interaction between anemia and blood levels of iron, zinc, copper, cadmium and lead in children Indian J Pediatr 2007 74 9 827 830 10.1007/s12098-007-0147-2 TURGUT S, POLAT A, INAN M, et al .Interaction between anemia and blood levels of iron, zinc, copper, cadmium and lead in children[J ] .Indian J P ediatr, 2007, 74(9):827-830.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

富勒醇纳米颗粒不同给药方式对放射性视网膜损伤安全性及有效性评价

新型冠状病毒肺炎患者转氨酶水平的Meta分析

粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响

一氧化氮对红细胞携氧特性的作用及体内吸入对小鼠耐缺氧能力的影响

粪便细菌移植对肝性脑病大鼠肝功能及血氨的影响