司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性

田中华; 朱深银; 常淑芳; 李雪梅; 唐琴; 郑晓英; 郝兰; 王志刚

doi:10.16016/j.1000-5404.201804069

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司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性

基础医学 | 更新时间：2026-01-09

- 司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性
- Sonodynamic cytotoxicity of sparfloxacin on rheumatoid arthritis fibroblast-like synoviocytes in vitro
- 第三军医大学学报 2018年40卷第19期页码：1748-1752,1761
- 作者机构：
  
  1.400016 重庆，重庆医科大学附属第一医院药学部
  2.400010 重庆，重庆医科大学超声分子影像研究所
  3.400010 重庆，重庆医科大学附属第二医院妇产科
- 作者简介：
  
  [ 朱深银，E-mail：zhushenyin0486@sina.com ZHU Shenyin, E-mail: zhushenyin0486@sina.com
- 基金信息：
  
  国家自然科学基金面上项目(81572558);重庆市研究生科研创新项目(CYS16127)
- DOI：10.16016/j.1000-5404.201804069
  中图分类号： R593.22; R454.3; R318.5
- 收稿：2018-04-11，
  
  修回：2018-05-18，
  
  纸质出版：2018-10-15
- 稿件说明：
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田中华, 朱深银, 常淑芳, 等. 司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性[J]. 第三军医大学学报, 2018,40(19):1748-1752,1761.

Zhonghua TIAN, Shenyin ZHU, Shufang CHANG, et al. Sonodynamic cytotoxicity of sparfloxacin on rheumatoid arthritis fibroblast-like synoviocytes in vitro[J]. Journal of Third Military Medical University, 2018, 40(19): 1748-1752,1761.
田中华, 朱深银, 常淑芳, 等. 司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性[J]. 第三军医大学学报, 2018,40(19):1748-1752,1761. DOI： 10.16016/j.1000-5404.201804069.

Zhonghua TIAN, Shenyin ZHU, Shufang CHANG, et al. Sonodynamic cytotoxicity of sparfloxacin on rheumatoid arthritis fibroblast-like synoviocytes in vitro[J]. Journal of Third Military Medical University, 2018, 40(19): 1748-1752,1761. DOI： 10.16016/j.1000-5404.201804069.

摘要

目的

探讨司帕沙星介导声动力疗法对类风湿关节炎成纤维样滑膜细胞的损伤作用，并阐明其可能的作用机制。

方法

人类风湿关节炎成纤维样滑膜细胞MH7A经司帕沙星(浓度1.00 μmol/L)处理后，采用超声辐照(声强1.0 W/cm

，辐照60 s)。实验分为对照组、司帕沙星组、超声组和声动力(司帕沙星+超声)组。MTT法和流式细胞仪检测MH7A细胞的损伤情况。多功能荧光酶标仪测定细胞内活性氧物质产生情况。

结果

声动力组细胞活力显著低于对照组(

s0.01)，细胞凋亡率显著高于对照组(

0.01)。与对照组相比，声动力疗法显著地诱导MH7A细胞内产生过量活性氧物质(

0.01)。与活性氧物质清除剂甘露醇相比，活性氧物质清除剂组氨酸有效地阻断声动力疗法诱导MH7A细胞活力降低(

s0.01)、细胞凋亡增加(

0.01)以及细胞内活性氧物质产生(

0.01)。

结论

司帕沙星介导声动力疗法显著地诱导MH7A细胞损伤，其机制可能与诱导细胞内产生过量活性氧物质有关。

Abstract

Objective

To investigate the impairing effect of sparfloxacin (SPFX)-mediated sonodynamic therapy (SDT) on rheumatoid arthritis fibroblast-like synoviocytes and further explore its potential mechanism.

Methods

Human rheumatoid arthritis fibroblast-like synoviocyte cell line MH7A was incubated with 1.00 μmol/L SPFX

and then the cells were exposed to ultrasound irradiation (1.0 W/cm

60 s). The cells were randomly divided into control group

SPFX group

ultrasound group and SDT (SPFX+ ultrasound) group. The viability of cells was examined by MTT assay. Cell apoptosis was analyzed using flow

cytometry. The production of intracellular reactive oxygen species (ROS) was detected by fluorescence microplate reader.

Results

SDT resulted in significantly lower cell viability (

0.01)

higher apoptotic rate (

0.01)

and excessive intracellular ROS (

0.01) in MH7A cells when compared with the untreated control cells. Moreover

compared with ROS scavenger D-mannitol

ROS scavenger L-histidine could significantly inhibit the loss of cell viability (

0.01)

increase of cell apoptosis (

0.01) and production of intracellular ROS (

0.01) induced by SPFX-mediated SDT.

Conclusion

SPFX-mediated SDT shows cytotoxic effects on MH7A cells

which may be related to the excessive production of intracellular ROS.

关键词

Keywords

references

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