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新型血红蛋白代用品对创伤失血性休克大鼠重要器官功能的保护作用
Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats
- 第三军医大学学报 2018年40卷第19期 页码：1729-1734
- 作者机构：
400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)第二研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
[ 李涛，E-mail：LT200132@163.com LI Tao, E-mail: LT200132@163.com
- 基金信息：国家自然科学基金青年科学基金(81700429);本研究与医药公司不存在商业利益关系，医药公司在实验设计及实施、数据处理、文章书写和发表等方面未给予任何资助
DOI：10.16016/j.1000-5404.201803174
中图分类号： R641;R657.62;R973.5收稿：2018-03-15，
修回：2018-07-02，
纸质出版：2018-10-15
- 稿件说明：
移动端阅览
朱娱, 田昆仑, 吴跃, 等. 新型血红蛋白代用品对创伤失血性休克大鼠重要器官功能的保护作用[J]. 第三军医大学学报, 2018,40(19):1729-1734.
Yu ZHU, Kunlun TIAN, Yue WU, et al. Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats[J]. Journal of Third Military Medical University, 2018, 40(19): 1729-1734.
朱娱, 田昆仑, 吴跃, 等. 新型血红蛋白代用品对创伤失血性休克大鼠重要器官功能的保护作用[J]. 第三军医大学学报, 2018,40(19):1729-1734. DOI： 10.16016/j.1000-5404.201803174.
Yu ZHU, Kunlun TIAN, Yue WU, et al. Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats[J]. Journal of Third Military Medical University, 2018, 40(19): 1729-1734. DOI： 10.16016/j.1000-5404.201803174.
摘要
目的
2
观察一种新型血红蛋白代用品，产品代号YQ，对非控失血性休克大鼠重要器官的保护作用。
方法
2
100只SD大鼠[雌雄各半，12周龄，体质量(220±20)g
]
随机分为(
n
=20)：0.5%YQ组、2%YQ组、5%YQ组、乳酸林格氏液(lactate Ringer，LR)对照组和全血组。采用切断脾动脉分支方法构建非控失血性休克模型，观察不同浓度YQ在不同复苏阶段(复苏血压维持50、70、80 mmHg，复苏后1、2 h等)对重要脏器(肝、肾)血流量、组织氧分压、器官功能及血气的影响。
结果
2
在不同复苏阶段，与LR对照组比较：①3个YQ浓度均可增加大鼠的PaO
2
和SaO
2
。复苏2 h后2%YQ[(98.8±15.9)mmHg
]
和5%YQ组[(98.6±14.1) mmHg
]
对PaO
2
的作用明显优于LR对照组[(68.6±5.6)mmHg
]
(
P
<
0.01)；2%YQ[(92.1±2.5) mmHg
]
和5%YQ组[(93.6±2.4) mmHg
]
对SaO
2
的作用明显优于LR对照组[(70.6±8.5)mmHg
]
(
P
<
0.01)，接近全血[(94.3±3.3) mmHg
]
。②3个YQ浓度均可增加经皮氧分压，其中5%YQ组效果最好，复苏2 h后其作用[(80.4±5.9) mmHg
]
接近全血组[(80.0±7.3) mmHg
]
，与LR对照组比较[(59.1±1.6) mmHg
]
，有显著性差异(
P
<
0.01)。③3个YQ浓度均可增加大鼠的肝肾血流量，其中5%YQ组效果最好，复苏2 h后其作用[肝：(218.7±13.8) U/min；肾：(187.4±10.9) U/min
]
接近全血组[肝：(220.4±18.2) U/min；肾：(179.2±16.3) U/min
]
，明显优于LR对照组[肝：(171.4±20.4) U/min；肾：(169.4±4.7) U/min
]
。④3个YQ浓度组均可降低大鼠AST、ALT、BUN和Crea水平。与LR对照组比较[AST：(209.6±15.6) U/L；ALT：(54.9±3.6) U/L；BUN：(10.8±2.5) mmol/L；Crea：(47.7±4.4) μmol/L
]
，复苏2 h后5%YQ组的肝肾功能[AST：(149.8±13.7)U/L；ALT：(45.9±4.2) U/L；BUN：(9.7±1.8) mmol/L；Crea：(25.8±2.1) μmol/L
]
明显减低。
结论
2
YQ通过改善肝、肾器官功能从而发挥对非控制失血性休克大鼠的保护作用。
Abstract
Objective
2
To investigate the effects of a new type hemoglobin substitute (product code YQ) on main organ functions in uncontrolled hemorrhagic shock rats.
Methods
2
A total of 100 SD rats (50 males and 50 females
at an age of 12 weeks
body weight 220±20 g) were randomly divided into 5 groups: 0.5%YQ
2.0%YQ
5%YQ
lactate Ringer's solution (LR) group
and whole blood group
with 20 rats in each group. After the uncontrolled hemorrhagic shock model was established in the rats by cutting off the splenic artery branch
the effects of different concentrations of YQ on vital organ blood flow (liver and kidneys)
functions of the liver and kidneys
and transcutaneous partial pressures of oxygen and blood gases were tested at vary resuscitation stages (resuscitation being maintained at 50
70 and 80 mmHg
in 1 and 2 h after resuscitation).
Results
2
① Compared with LR
all 3 doses of YQ increased PaO
2
and SaO
2
at different resuscitation stages; At 2 h post-resuscitation stage
the 2% YQ (98.8±15.9 mmHg) and 5% YQ groups (98.6±14.1 mmHg) showed better effects on PaO
2
than the LR group (68.6±5.6 mmHg
P
<
0.01)
while so were their effects on SaO
2
(92.1±2.5 and 93.6±2.4
vs
70.6±8.5 mmHg
P
<
0.01)
which was similar to that of the whole blood group (94.3±3.3 mmHg). ② All the 3 doses of YQ enhanced the transcutaneous oxygen pressures at different resuscitation stages
and 5% YQ presented the best effect
with its effect closed to that of the whole blood group at 2 h post-resuscitation stage (80.4±5.9
vs
80.0±7.3 mmHg)
while significantly higher than that of the LR group (59.1±1.6 mmHg
P
<
0.01). ③ The liver and kidney blood flows were elevated in all the 3 YQ groups at different resuscitation stages. Among them
the 5% YQ had the best effect
with the effects equivalent to those of the whole blood group at 2 h post-resuscitation stage (liver: 218.7±13.8
vs
220.4±18.2 U/min; kidneys: 187.4±10.9
vs
179.2±16.3 U/min) and better than those of the LR group (liver: 171.4±20.4 U/min; kidneys: 169.4±4.7 U/min). ④ The levels of AST
ALT
BUN and Crea were all reduced in the 3 YQ groups at different resuscitation stages. The 5% YQ group had significantly decreased AST (209.6±15.6
vs
149.8±13.7 U/L)
ALT (54.9±3.6
vs
45.9±4.2 U/L)
BUN (10.8±2.5
vs
9.7±1.8 mmol/L) and Crea (47.7±4.4
vs
25.8±2.1 μmol/L) when compared with the LR group at 2 h post-resuscitation stage.
Conclusion
2
YQ exerts protective effect on uncontrolled hemorrhagic shock via improving liver and kidney functions.
关键词
Keywords
references
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