原头蚴成囊过程中内参基因的确定及水通道候选基因的转录表达

王芬; 刘许诺; 吴宏烨; 李锴; 范俊杰; 叶彬

doi:10.16016/j.1000-5404.201803049

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原头蚴成囊过程中内参基因的确定及水通道候选基因的转录表达

基础医学 | 更新时间：2026-01-09

- 原头蚴成囊过程中内参基因的确定及水通道候选基因的转录表达
- Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins
- 第三军医大学学报 2018年40卷第17期页码：1533-1541
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81672045);国家自然科学基金面上项目(30972567);国家自然科学基金面上项目(30371258)
- DOI：10.16016/j.1000-5404.201803049
  中图分类号：
- 收稿：2018-03-12，
  
  纸质出版：2018-09-15
- 稿件说明：
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王芬, 刘许诺, 吴宏烨, 等. 原头蚴成囊过程中内参基因的确定及水通道候选基因的转录表达[J]. 第三军医大学学报, 2018,40(17):1533-1541.

Fen WANG, Xunuo LIU, Hongye WU, et al. Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins[J]. Journal of Third Military Medical University, 2018, 40(17): 1533-1541.
王芬, 刘许诺, 吴宏烨, 等. 原头蚴成囊过程中内参基因的确定及水通道候选基因的转录表达[J]. 第三军医大学学报, 2018,40(17):1533-1541. DOI： 10.16016/j.1000-5404.201803049.

Fen WANG, Xunuo LIU, Hongye WU, et al. Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins[J]. Journal of Third Military Medical University, 2018, 40(17): 1533-1541. DOI： 10.16016/j.1000-5404.201803049.

摘要

Abstract

Objective

To select suitable reference genes for detecting transcriptional expression profiles of aquaporins genes of

Echinococcus granulosus

(

AQPs) during the development of hydatid cysts from protoscoleces.

Methods

geNorm software was used to evaluate the stability of 5 candidate reference genes

including

ND1

ATP6

elp

actin

and

Act

Ⅱ

and then RT-qPCR was employed to detect the transcriptional expression level of

AQP in

in vitro

protoscoleces and in mice during the development of hydatid cysts from protoscoleces.

Results

Actin

and

Act

Ⅱ were optimal for detecting transcriptional level of

AQP gene in

in vitro

protoscoleces by RT-qPCR. While

ATP6

and

ND1

were optimal for the transcriptional level of the

AQP3 genes in the germinal cells of cyst walls of hydatids developed from protoscoleces

in vivo

. The transcriptional levels of

AQP3 and

AQP9 reached the highest in protoscoleces after 3 days of culture

and then were in

trend of decreasing with the time elapse

with significant difference in different time points (

0.05). The transcriptional expression of

AQP4 was the highest in protoscoleces after 25 days' culture (

0.05)

and comparatively lower in the days 3

15 and 35

though without notably difference (

0.05). In

in vivo

study

the transcriptional levels of

AQP3

AQP4 and

AQP9 were the highest in protoscoleces (

0.05)

and were quite low in 3 to 7 months after the protoscoleces inoculation in the abdominal cavities of mice

but there were no statistical differences (

0.05). The expression levels of

AQP

AQP1

AQPFA-CHIP

and

AQPAnG were very low and even hardly detected in both the protoscoleces and the germinal cells of cyst walls of hydatids developed from protoscoleces.

Conclusion

The optimal reference genes for transcriptional expression are identified by geNorm during the development of hydatid cysts from protoscoleces. The transcriptional levels of

AQPs genes are very low in germinal cells of cyst walls of hydatids developed from protoscoleces

which may be related to the formation or accumulation of hydatid fluid.

关键词

Keywords

references

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