慢性脑低灌注致大鼠持续性学习记忆受损及海马神经血管单元改变

黄燕; 何婧; 向阳; 王志强; 杜果; 王庆松

doi:10.16016/j.1000-5404.201803044

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慢性脑低灌注致大鼠持续性学习记忆受损及海马神经血管单元改变

基础医学 | 更新时间：2026-01-09

- 慢性脑低灌注致大鼠持续性学习记忆受损及海马神经血管单元改变
- Chronic cerebral hypoperfusion facilitates lasting learning and memory impairment and induces hippocampal neurovascular unit dysfunction in rats
- 第三军医大学学报 2018年40卷第17期页码：1561-1567
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金(81601112);四川省卫计委科学研究项目(16PJ014)
- DOI：10.16016/j.1000-5404.201803044
  中图分类号：
- 收稿：2018-03-06，
  
  纸质出版：2018-09-15
- 稿件说明：
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黄燕, 何婧, 向阳, 等. 慢性脑低灌注致大鼠持续性学习记忆受损及海马神经血管单元改变[J]. 第三军医大学学报, 2018,40(17):1561-1567.

Yan HUANG, Jing HE, Yang XIANG, et al. Chronic cerebral hypoperfusion facilitates lasting learning and memory impairment and induces hippocampal neurovascular unit dysfunction in rats[J]. Journal of Third Military Medical University, 2018, 40(17): 1561-1567.
黄燕, 何婧, 向阳, 等. 慢性脑低灌注致大鼠持续性学习记忆受损及海马神经血管单元改变[J]. 第三军医大学学报, 2018,40(17):1561-1567. DOI： 10.16016/j.1000-5404.201803044.

Yan HUANG, Jing HE, Yang XIANG, et al. Chronic cerebral hypoperfusion facilitates lasting learning and memory impairment and induces hippocampal neurovascular unit dysfunction in rats[J]. Journal of Third Military Medical University, 2018, 40(17): 1561-1567. DOI： 10.16016/j.1000-5404.201803044.

摘要

Abstract

Objective

To investigate the characteristics of changes in hippocampal neurovascular unit in rats after chronic cerebral hypoperfusion (CCH)

and explore the relationship of the changes of the unit with learning and memory impairment induced by CCH.

Methods

A total of 44 male adult SD rats (220~250 g) were randomly

divided into: CCH group (

=24) and control group (

=20). CCH was induced by permanent ligation of the bilateral carotid artery. Open field test

object recognition test and Morris water maze test were used to evaluate exploratory behavior and learning and memorial function at 4 and 12 weeks after the operation for each group of rats. Then double immunofluorescence labeling was used to observe the co-expression of aquaporin-4 (AQP4) in astrocytic endfeet and vascular basement membrane of collagen Ⅳ in the hippocampus. Furthermore

transmission electron microscopy was used to observe the ultrastructure of neurovascular unit in the hippocampus.

Results

Compared with the control group

in CCH groups in 4 and 12 weeks after the operation

① the object discrimination index was decreased clearly (0.86± 0.14

0.71±0.16

0.05; 0.83±0.19

0.64±0.10

0.05)

the escape latencies of place navigation test and spatial probe test were prolonged notably (

0.05); ② the expression of AQP4 in astrocytic endfeet (1.94±0.80

1.10±0.41

0.05; 2.05±0.51

0.96±0.50

0.01)

that of collagen Ⅳ in vascular basement membrane (6.90±2.42

4.25±1.01

0.05; 6.63±2.20

3.66±1.22

0.01)

and the co-expression ratio of them (0.94±0.02

0.91±0.01

0.01; 0.91±0.01

0.89±0.01

0.01) were all decreased obviously; ③ the ultrastructure of neurovascular unit displayed that astrocytic endfeet were separated from the vascular basement membrane

and gliovascular interface and neurovascular unit was damaged in the hippocampus.

Conclusion

CCH facilitates the lasting learning and memory impairment

which may be associated with neurovascular unit dysfunction.

关键词

Keywords

references

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