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DFMG调节血管新生对APOE-/-小鼠动脉粥样硬化斑块稳定性的影响
DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis
- 第三军医大学学报 2018年40卷第17期 页码：1554-1560
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81370382);湖南省自然科学基金(14JJ2059)
DOI：10.16016/j.1000-5404.201803026
中图分类号：收稿：2018-03-04，
纸质出版：2018-09-15
- 稿件说明：
移动端阅览
柏萍娟, 王如波, 曾菲, 等. DFMG调节血管新生对APOE-/-小鼠动脉粥样硬化斑块稳定性的影响[J]. 第三军医大学学报, 2018,40(17):1554-1560.
Pingjuan BO, Rubo WANG, Fei ZENG, et al. DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis[J]. Journal of Third Military Medical University, 2018, 40(17): 1554-1560.
柏萍娟, 王如波, 曾菲, 等. DFMG调节血管新生对APOE-/-小鼠动脉粥样硬化斑块稳定性的影响[J]. 第三军医大学学报, 2018,40(17):1554-1560. DOI： 10.16016/j.1000-5404.201803026.
Pingjuan BO, Rubo WANG, Fei ZENG, et al. DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis[J]. Journal of Third Military Medical University, 2018, 40(17): 1554-1560. DOI： 10.16016/j.1000-5404.201803026.
摘要
Abstract
Objective
2
To investigate the effect of 7-difluoromethoxy-5
4′-dimethoxygenistein (DFMG) on the angiogenesis and plaque stability in the atherosclerosis model of the Apolipoprotein E knockout mice (ApoE
-/-
).
Methods
2
A total of 20 ApoE
-/-
mice were randomly divided into 4 groups (
n
=5): model group
solvent (DMSO) group
DFMG group
and lovastatin group. And 5 C57BL/6 mice were assigned to the blank control group. The bla
nk control group was fed with normal diet and other 4 groups were fed with high-fat diet to induce atherosclerosis model. DFMG of 10 mg/(kg·d) was given to the mice of the DFMG group
and lovastatin 5 mg/(kg·d) to those of the lovastatin group. After 16 weeks
lipid content in the mice serum was measured by automatic biochemical analyzer. The lipid deposition in the gross thoracic aorta was evaluated by oil red O staining. The plaque stability in the thoracic aorta was measured by Masson staining. The angiogenesis was measured by the immunohistochemical staining with VEGF and vWF antibodies. The protein levels of TLR4 in the thoracic aorta were detected by Western blotting.
Results
2
DFMG decreased the ratio of atherosclerotic plaque to intraluminal diameter (
P
<
0.05)
reduced the levels of plasma lipids
including LDL
VLDL
TG and CHOL (
P
<
0.05)
and attenuated the amount of lipid red stains in the thoracic aorta (
P
<
0.05)
increased collagen in the plaque (
P
<
0.05) and lowered the expression of VEGF
vWF and TLR4 in the thoracic aorta (
P
<
0.05).
Conclusion
2
DFMG may inhibit the angiogenesis and maintain the stability of atherosclerotic plaque in the atherosclerotic ApoE
-/-
mice.
关键词
Keywords
references
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