β-连环素互作蛋白1参与PPARγ转录活性调控及在高糖诱导系膜细胞表型转化中的作用

魏毅; 于家伟; 张钧; 曾薇; 冯兵

doi:10.16016/j.1000-5404.201803011

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β-连环素互作蛋白1参与PPARγ转录活性调控及在高糖诱导系膜细胞表型转化中的作用

基础医学 | 更新时间：2026-01-09

- β-连环素互作蛋白1参与PPARγ转录活性调控及在高糖诱导系膜细胞表型转化中的作用
- Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose
- 第三军医大学学报 2018年40卷第17期页码：1542-1547
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81370820);国家自然科学基金青年科学基金(81400737)
- DOI：10.16016/j.1000-5404.201803011
  中图分类号：
- 收稿：2018-03-02，
  
  纸质出版：2018-09-15
- 稿件说明：
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魏毅, 于家伟, 张钧, 等. β-连环素互作蛋白1参与PPARγ转录活性调控及在高糖诱导系膜细胞表型转化中的作用[J]. 第三军医大学学报, 2018,40(17):1542-1547.

Yi WEI, Jiawei YU, Jun ZHANG, et al. Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose[J]. Journal of Third Military Medical University, 2018, 40(17): 1542-1547.
魏毅, 于家伟, 张钧, 等. β-连环素互作蛋白1参与PPARγ转录活性调控及在高糖诱导系膜细胞表型转化中的作用[J]. 第三军医大学学报, 2018,40(17):1542-1547. DOI： 10.16016/j.1000-5404.201803011.

Yi WEI, Jiawei YU, Jun ZHANG, et al. Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose[J]. Journal of Third Military Medical University, 2018, 40(17): 1542-1547. DOI： 10.16016/j.1000-5404.201803011.

摘要

Abstract

Objective

To explore the roles of β-catenin-interacting protein 1 (ICAT) in regulating peroxisome proliferator-activated receptor gamma (PPARγ) transcriptional activity and inducing cell phenotypic transition in human mesangial cells (HMCs) cultured in high glucose (HG).

Methods

HMCs were cultured in either 5.5 (NC) or 30 (HG) mmol/L glucose medium. RT-PCR was used to detect the expression of PPARγ

ICAT and β-catenin in the cells. The protein levels of PPARγ

ICAT and β-catenin were detected by Western blotting. Cell phenotype transition of HMCs was detected by the expression levels of α-smooth muscular actin (α-SMA). And CCK-8 assay was used to measure cell proliferation.

Results

Compared with NC

HG induced the expression of α-SMA and promoted the proliferation of HMCs. Though HG showed no significant influence on PPARγ expression in HMCs (

0.05)

it lowered the transcriptional activity of PPARγ (

0.05)

inhibited the expression of ICAT but enhanced β-catenin. Over-expression of ICAT partially promoted the transcriptional activity of PPARγ (

0.05). Under normal glucose condition

knockdown of ICAT reduced the transcriptional activity of PPARγ (

0.01)

so was β-catenin knockdown (

0.01). However

under high glucose condition

over-expression of ICAT resulted in decreased expression of α-SMA and reduced proliferation of HMCs.

Conclusion

ICAT might be one of potential molecules in regulation of PPARγ transcriptional activity

and it also participates in mediating the cell phenotypic transition of HMCs induced by high glucose.

关键词

Keywords

references

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