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不同形态电纺纤维膜的制备及其亲水性、降解和对成骨细胞增殖和分化影响的比较
Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation
- 第三军医大学学报 2018年40卷第14期 页码：1295-1300
- 作者机构：
4000015 重庆，重庆医科大学附属口腔医院修复科，口腔疾病与生物医学重庆市重点实验室，重庆市高校市级口腔生物医学工程重点实验室
- 作者简介：
[吴小红，E-mail: hiwxh@hotmail.com WU Xiaohong, E-mail: hiwxh@hotmail.com
- 基金信息：2016年重庆高校创新团队建设计划资助项目(CXTDG201602006);重庆市高校市级口腔生物医学工程重点实验室资助项目;重庆市科委资助项目(cstc2017jcyjAX0215);重庆市渝中区科技计划项目(20170117)
DOI：10.16016/j.1000-5404.201803003
中图分类号： R322.71;R329-33;R329.24收稿：2018-03-01，
修回：2018-04-17，
纸质出版：2018-07-30
- 稿件说明：
移动端阅览
张一弓, 张琼月, 邵华英, 等. 不同形态电纺纤维膜的制备及其亲水性、降解和对成骨细胞增殖和分化影响的比较[J]. 第三军医大学学报, 2018,40(14):1295-1300.
Yigong ZHANG, Qiongyue ZHANG, Huaying SHAO, et al. Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation[J]. Journal of Third Military Medical University, 2018, 40(14): 1295-1300.
张一弓, 张琼月, 邵华英, 等. 不同形态电纺纤维膜的制备及其亲水性、降解和对成骨细胞增殖和分化影响的比较[J]. 第三军医大学学报, 2018,40(14):1295-1300. DOI： 10.16016/j.1000-5404.201803003.
Yigong ZHANG, Qiongyue ZHANG, Huaying SHAO, et al. Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation[J]. Journal of Third Military Medical University, 2018, 40(14): 1295-1300. DOI： 10.16016/j.1000-5404.201803003.
摘要
目的
2
制备微观下不同形态的聚己内酯(polycaprolactone，PCL)电纺纳米纤维膜并比较其亲水性、降解速度和对成骨细胞增殖、分化的影响。
方法
2
调整电纺参数制备出不同形态的电纺纤维膜，用扫描电子显微镜及倒置相差显微镜观察其形态，并通过可视接触角测试系统测试不同膜的表面接触角比较其亲水性，测量失重率评估其降解速度，细胞增殖实验、ALP活性实验和qRT-PCR实验评估其对成骨细胞增殖和分化的影响。
结果
2
15%、10%、8%组PCL所制得的电纺纤维膜在电子扫描显微镜500倍和倒置相差显微镜100倍下分别呈现出纯丝状、含有少量串珠的丝状、大量串珠状。3组电纺纤维膜的亲水性和对成骨细胞增殖的影响无明显差异；降解速度：纯丝组＜少量串珠组＜大量串珠组；成骨细胞分化的程度：纯丝组＞少量串珠组＞大量串珠组。
结论
2
调节电纺参数可制备不同形态的电纺纤维膜，纯丝状的电纺纤维膜较串珠状电纺纤维膜降解慢且更有利于成骨细胞的分化。
Abstract
Objective
2
To prepare electrospinning nanofiber membranes with different microscopic morphologies and compare their hydrophilicity
degradation
and influences on the proliferation and differentiation of osteoblasts.
Methods
2
Different electrospinning nanofiber membranes were prepared by adjusting the parameters
and the microscopic morphologies of the membranes were observed using scanning electron microscope and inverted phase-contrast microscope. The hydrophilicity and degradation of these membranes and their influences on the proliferation and differentiation of osteoblasts were analyzed using a visual contact angle test system
weight loss test
cell proliferation assay
ALP activity assay and qRT-PCR.
Results
2
Under scanning electron microscope (×500) and inverted phase-contrast microscope (×100)
the electrospinning nanofiber membranes prepared with 15%
10%
and 8% PCL showed a filamentous morphology
containing a small amount of beads
and a predominantly bead-like morphology
respectively. The 3 membranes exhibited similar hydrophilicity with also similar influences on osteoblast proliferation. The bead-like membrane had the highest degradation rate
followed by the bead-containing filamentous membrane and then by the filamentous membrane. The degree of osteoblasts differentiation on the 3 membranes was lowered in the order filamentous membrane
bead-containing filamentous membrane and bead-like membrane.
Conclusion
2
Electrospinning membrane with different microscopic morphologies can be prepared by adjusting the electrospinning parameters. Compared with the bead-containing and bead-like electrospinning membranes
the filamentous membrane is characterized by a slower degradation rate and a better capacity to promote the differentiation of osteoblasts.
关键词
Keywords
references
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