两种脱矿液在粘接前表面处理牙本质时的脱矿动力学曲线比较及其对胶原酶解的影响

寇婷婷; 熊宇; 崔岳; 蔡江文; 朱璨; 周继祥

doi:10.16016/j.1000-5404.201802122

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两种脱矿液在粘接前表面处理牙本质时的脱矿动力学曲线比较及其对胶原酶解的影响

生物医学工程 | 更新时间：2026-01-09

- 两种脱矿液在粘接前表面处理牙本质时的脱矿动力学曲线比较及其对胶原酶解的影响
- Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding
- 第三军医大学学报 2018年40卷第12期页码：1155-1160
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院口腔科
- 作者简介：
  
  [ 周继祥，E-mail：Zhoujx6518@sina.com ZHOU Jixiang, E-mail:Zhoujx6518@sina.com
- 基金信息：
  
  国家自然科学基金面上项目(81671022)
- DOI：10.16016/j.1000-5404.201802122
  中图分类号： R313;R783.1;R988.2
- 收稿：2018-02-22，
  
  修回：2018-03-27，
  
  纸质出版：2018-01
- 稿件说明：
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寇婷婷, 熊宇, 崔岳, 等. 两种脱矿液在粘接前表面处理牙本质时的脱矿动力学曲线比较及其对胶原酶解的影响[J]. 第三军医大学学报, 2018,40(12):1155-1160.

Tingting KOU, Yu XIONG, Yue CUI, et al. Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding[J]. Journal of Third Military Medical University, 2018, 40(12): 1155-1160.
寇婷婷, 熊宇, 崔岳, 等. 两种脱矿液在粘接前表面处理牙本质时的脱矿动力学曲线比较及其对胶原酶解的影响[J]. 第三军医大学学报, 2018,40(12):1155-1160. DOI： 10.16016/j.1000-5404.201802122.

Tingting KOU, Yu XIONG, Yue CUI, et al. Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding[J]. Journal of Third Military Medical University, 2018, 40(12): 1155-1160. DOI： 10.16016/j.1000-5404.201802122.

摘要

目的

比较37%磷酸和17% EDTA对人牙本质的脱矿效率，并评价不同脱矿时间对人牙本质中Ⅰ型胶原基质降解速率的影响。

方法

制取等量人牙本质粉，分别以37%磷酸(Pi)与17% EDTA脱矿处理0 s(对照组)、10 s、15 s、30 s、60 s、120 s、72 h后离心荡洗。检测计算两种脱矿液的脱矿效率及胶原溶脱率并绘制动力学曲线。1 g/L Ⅰ型胶原酶溶液300 μL酶解样本沉淀，于1、7 d用羟脯胺酸(HYP)试剂盒测定并绘制胶原降解变化趋势图。利用Pearson相关系数分析脱矿总量与胶原溶脱、酶解1 d胶原降解量、酶解7 d胶原降解量及增量之间的相关性。

结果

磷酸组15 s即脱矿显著，胶原溶脱30 s明显增高(

0.05)，胶原降解量各时间点差异无统计学意义，降解增量15 s开始降低(

0.05)，脱矿量与胶原溶脱量及降解增量呈显著相关(

=0.864，

=-0.713，

0.05)。EDTA组30 s明显脱矿，胶原溶脱较少，且120s内各时间点差异无统计学意义，胶原降解量30 s达峰值，随后各时间点胶原降解量明显降低(

0.05)，降解增量60s开始降低(

0.05)，脱矿量与胶原溶脱量呈显著相关(

=0.894，

0.05)。

结论

磷酸脱矿效率迅速、高效，胶原溶脱率较高，稳定性较好；EDTA脱矿效率缓慢、持续，胶原溶脱率较低，稳定性尚可。临床进行牙本质粘结前表面处理牙本质时，以37%磷酸酸蚀10~15 s或17% EDTA表面处理60~90 s为较合理。

Abstract

Objective

To compare the demineralization efficiency of 37% phosphoric acid and 17% EDTA in human dentin and evaluate the effect of different etching time on the degradation rate of type Ⅰ collagen matrix in human dentin.

Methods

Equal amounts of human dentin powder were demineralized with 37% phosphoric acid and 17% EDTA for 0 s (control group)

60 and 120 s

or 72 h. After centrifugation and washing

the samples were analyzed for concentrations of Ca

and P

in the washing solution using an automatic biochemical analyzer. The demineralization curves were drawn and the demineralization efficiency of the 2 demineralization solutions was compared. The precipitates in each group were enzymatically hydrolyzed on day 1 and day 7 with 300 μL type Ⅰ collagenase solution (1 g/L). Pearson correlation coefficient was used to analyze the correlation among the total demineralization

collagen dissolution and collagen degradation.

Results

Treatment with 37% phosphoric acid solution for 15 s caused obvious demineralization in the dentin samples

and collagen dissolution increased significantly at 30 s (

0.05); collagen degradation levels did not show significant changes over time

and the increment of collagen degradation began to decrease significantly at 15 s (

0.05). The amount pf demineralization was strongly correlated with collagen dissolution (

=0.864

0.05) and degradation increment (

=-0.713

0.05)

. Treatment with 17% EDTA for 30 s resulted in obvious demineralization of dentin but less obvious collagen dissolution

which showed no significant changes within 120 s of treatment (

0.05); The peak level of collagen degradation occurred at 30 s followed by significant and progressive decreases (

0.05)

and the degradation increment began to decrease significantly at 60 s (

0.05). The amount of demineralization was strongly correlated with collagen dissolution (

=0.894

0.05).

Conclusion

Phosphoric acid results in rapid and efficient demineralization of dentin with a high collagen dissolution rate

and the dentin collagen matrix has a good stability after demineralization. EDTA has a relatively low efficiency of demineralization and collagen dissolution

and the stability of dentin collagen matrix after demineralization is acceptable. Clinically

both 37% phosphoric acid with an etching time of 10~15 s and 17% EDTA acid with an etching time of 60~90 s can be optimal for dentin treatment before dentin bonding.

关键词

Keywords

references

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