浏览全部资源
扫码关注微信
辣椒碱抑制分化因子1表达以及核转移提高骨肉瘤对顺铂敏感性
Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1
- 第三军医大学学报 2018年40卷第16期 页码：1492-1499
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81272039);重庆市科学技术委员会重点项目(CSTC2017jcyjBX0017)
DOI：10.16016/j.1000-5404.201802097
中图分类号：收稿：2018-02-17，
纸质出版：2018-08-30
- 稿件说明：
移动端阅览
余畅, 王旸, 熊梅, 等. 辣椒碱抑制分化因子1表达以及核转移提高骨肉瘤对顺铂敏感性[J]. 第三军医大学学报, 2018,40(16):1492-1499.
Chang YU, Yang WANG, Mei XIONG, et al. Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1[J]. Journal of Third Military Medical University, 2018, 40(16): 1492-1499.
余畅, 王旸, 熊梅, 等. 辣椒碱抑制分化因子1表达以及核转移提高骨肉瘤对顺铂敏感性[J]. 第三军医大学学报, 2018,40(16):1492-1499. DOI： 10.16016/j.1000-5404.201802097.
Chang YU, Yang WANG, Mei XIONG, et al. Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1[J]. Journal of Third Military Medical University, 2018, 40(16): 1492-1499. DOI： 10.16016/j.1000-5404.201802097.
摘要
Abstract
Objective
2
To investigate the expression of inhibitor of differentiation 1 (Id1) in osteosarcoma tissues and evaluate the effect of capsaicin to enhance the chemosensitivity of osteosarcoma to cisplatin.
Methods
2
Immunohistochemistry was used to investigate Id1 expression levels in 12 osteosacroma tissue specimens and bone tissues from 12 patients with osteoarthritis.Three osteosarcoma cell lines (MG63
HOS and 143B) were treated with cisplatin and capsaicin
alone or in combination
and the expression levels of Id1
p-p65 and p-Smad1/5 in the cells were detected with Western blotting; immunofluorescence assay was used to investigate the location of Id1
and flow cytometry was used to analyze cell apoptosis in the treated cells.
Results
2
Osteosarcoma tissues expressed high levels of Id1 compared with osteoarthritis tissues.In the osteosarcoma cell lines
cisplain treatment induced an obvious up-regulation of Id1 expression (
P
<
0.05) and nuclear translocation of Id1
while treatment with capsaicin significantly reduced Id1 expression by suppressing p65 and Smad1/5 (
P
<
0.05).In cells treated with both capsaicin and cisplatin
capsaicin obviously reduced cisplatin-induced nuclear translocation of Id1 (
P
<
0.05) and significantly enhanced cisplatin-mediated cell apoptosis (
P
<
0.05).
Conclusion
2
Capsaicin can inhibit cisplatin-induced up-regulation and nuclear-translocation of Id1 and enhance cisplatin-mediated osteosarcoma cell apoptosis
and may thus serve as a potential agent for adjuvant chemotherapy of osteosarcoma.
关键词
Keywords
references
ISAKOFF M S BIELACK S S MELTZER P Osteosarcoma: current treatment and a collaborative pathway to success J Clin Oncol 2015 33 27 3029 3035 10.1200/JCO.2014.59.4895 ISAKOFF M S, BIELACK S S, MELTZER P, et al . Osteosarcoma: current treatment and a collaborative pathway to success[J ] . J Clin Oncol, 2015, 33(27): 3029-3035. DOI: 10.1200/JCO.2014.59.4895.
GIANFERANTE D M MIRABELLO L SAVAGE S A Germline and somatic genetics of osteosarcoma-connecting aetiology, biology and therapy Nat Rev Endocrinol 2017 13 8 480 491 10.1038/nrendo.2017.16 GIANFERANTE D M, MIRABELLO L, SAVAGE S A. Germline and somatic genetics of osteosarcoma-connecting aetiology, biology and therapy[J]. Nat Rev Endocrinol, 2017, 13(8): 480-491. DOI: 10.1038/nrendo.2017.16.
SZTURZ P WOUTERS K KIYOTA N Weekly low-dose versus three-weekly high-dose cisplatin for concurrent chemoradiation in locoregionally advanced non-nasopharyngeal head and neck cancer: A systematic review and meta-analysis of aggregate data Oncologist 2017 22 9 1056 1066 10.1634/theoncologist.2017-0015 SZTURZ P, WOUTERS K, KIYOTA N, et al . Weekly low-dose versus three-weekly high-dose cisplatin for concurrent chemoradiation in locoregionally advanced non-nasopharyngeal head and neck cancer: A systematic review and meta-analysis of aggregate data[J ] . Oncologist, 2017, 22(9): 1056-1066. DOI: 10.1634/theoncologist.2017-0015.
仇 超 康 权 迭 小红 Id1基因对人骨肉瘤细胞恶性逆转向成骨分化的影响 第三军医大学学报 2016 38 4 344 349 10.16016/j.1000-5404.201507046 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201507046 仇超, 康权, 迭小红, 等.Id1基因对人骨肉瘤细胞恶性逆转向成骨分化的影响[J].第三军医大学学报, 2016, 38(4): 344-349. DOI: 10.16016/j.1000-5404.201507046.
QIU C KANG Q DIE X H Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells J Third Mil Med Univ 2016 38 4 344 349 10.16016/j.1000-5404.201507046 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201507046 QIU C, KANG Q, DIE X H, et al . Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells[J ] . J Third Mil Med Univ, 2016, 38(4): 344-349. DOI: 10.16016/j.1000-5404.201507046.
HAO L LIAO Q TANG Q Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway Biochem Biophys Res Commun 2016 470 3 643 649 10.1016/j.bbrc.2016.01.090 HAO L, LIAO Q, TANG Q, et al . Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway[J ] . Biochem Biophys Res Commun, 2016, 470(3): 643-649. DOI: 10.1016/j.bbrc.2016.01.090.
ENDO-MUNOZ L CUMMING A SOMMERVILLE S Osteosarcoma is characterised by reduced expression of markers of osteoclastogenesis and antigen presentation compared with normal bone Br J Cancer 2010 103 1 73 81 10.1038/sj.bjc.6605723 ENDO-MUNOZ L, CUMMING A, SOMMERVILLE S, et al . Osteosarcoma is characterised by reduced expression of markers of osteoclastogenesis and antigen presentation compared with normal bone[J ] . Br J Cancer, 2010, 103(1): 73-81. DOI: 10.1038/sj.bjc.6605723.
周 栩 权 正学 罗 小辑 Id1基因在BMP-2维持椎间盘细胞软骨特性中的作用 第三军医大学学报 2015 37 20 2011 2016 10.16016/j.1000-5404.201502101 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201502101 周栩, 权正学, 罗小辑, 等. Id1基因在BMP-2维持椎间盘细胞软骨特性中的作用[J].第三军医大学学报, 2015, 37(20): 2011-2016. DOI: 10.16016/j.1000-5404. 201502101.
ZHOU X QUAN Z X LUO X J Role of Id1 gene in maintenance of cartilage phenotype in BMP-2-induced intervertebral disc cells J Third Mil Med Univ 2015 37 20 2011 2016 10.16016/j.1000-5404.201502101 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201502101 ZHOU X, QUAN Z X, LUO X J, et al . Role of Id1 gene in maintenance of cartilage phenotype in BMP-2-induced intervertebral disc cells[J ] . J Third Mil Med Univ, 2015, 37(20): 2011-2016. DOI: 10.16016/j.1000-5404.201502101.
HU H HAN H Y WANG Y L The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells Oncol Rep 2009 21 4 1053 1059 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JJ026522904 HU H, HAN H Y, WANG Y L, et al . The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells[J ] . Oncol Rep, 2009, 21(4): 1053-1059.
MERN D S HASSKARL J BURWINKEL B Inhibition of Id proteins by a peptide aptamer induces cell-cycle arrest and apoptosis in ovarian cancer cells Br J Cancer 2010 103 8 1237 1244 10.1038/sj.bjc.6605897 MERN D S, HASSKARL J, BURWINKEL B. Inhibition of Id proteins by a peptide aptamer induces cell-cycle arrest and apoptosis in ovarian cancer cells[J]. Br J Cancer, 2010, 103(8): 1237-1244. DOI:10.1038/sj.bjc.6605897.
CHENG Y J LEE Y C CHIU W C High Id1 expression, a generally negative prognostic factor, paradoxically predicts a favorable prognosis for adjuvant paclitaxel plus cisplatin therapy in surgically treated lung cancer patients Oncotarget 2014 5 22 11564 11575 10.18632/oncotarget.2595 CHENG Y J, L EE Y C, CHIU W C, et al . High Id1 expression, a generally negative prognostic factor, paradoxically predicts a favorable prognosis for adjuvant paclitaxel plus cisplatin therapy in surgically treated lung cancer patients[J ] . Oncotarget, 2014, 5(22): 11564-11575. DOI: 10.18632/oncotarget.2595.
MURASE R SUMIDA T KAWAMURA R Suppression of invasion and metastasis in aggressive salivary cancer cells through targeted inhibition of ID1 gene expression Cancer Lett 2016 377 1 11 16 10.1016/j.canlet.2016.04.021 MURASE R, SUMIDA T, KAWAMURA R, et al . Suppression of invasion and metastasis in aggressive salivary cancer cells through targeted inhibition of ID1 gene expression[J ] . Cancer Lett, 2016, 377(1): 11-16. DOI: 10.1016/j.canlet.2016.04.021.
KIM H CHUNG H KIM H J Id-1 regulates Bcl-2 and Bax expression through p53 and NF-kappaB in MCF-7 breast cancer cells Breast Cancer Res Treat 2008 112 2 287 296 10.1007/s10549-007-9871-6 KIM H, CHUNG H, KIM H J, et al . Id-1 regulates Bcl-2 and Bax expression through p53 and NF-kappaB in MCF-7 breast cancer cells[J ] . Breast Cancer Res Treat, 2008, 112(2): 287-296. DOI: 10.1007/s10549-007-9871-6.
LING Y X TAO J FANG S F Downregulation of Id1 by small interfering RNA in prostate cancer PC3 cells in vivo and in vitro Eur J Cancer Prev 2011 20 1 9 17 10.1097/CEJ.0b013e32833ebaa0 LING Y X, TAO J, FANG S F, et al . Downregulation of Id1 by small interfering RNA in prostate cancer PC3 cells in vivo and in vitro [J ] . Eur J Cancer Prev, 2011, 20(1): 9-17. DOI: 10.1097/CEJ.0b013e32833ebaa0.
LIN J GUAN Z WANG C Inhibitor of differentiation 1 contributes to head and neck squamous cell carcinoma survival via the NF-kappaB/survivin and phosphoinositide 3-kinase/Akt signaling pathways Clin Cancer Res 2010 16 1 77 87 10.1158/1078-0432.CCR-08-2362 LIN J, GUAN Z, WANG C, et al . Inhibitor of differentiation 1 contributes to head and neck squamous cell carcinoma survival via the NF-kappaB/survivin and phosphoinositide 3-kinase/Akt signaling pathways[J ] . Clin Cancer Res, 2010, 16(1): 77-87. DOI: 10.1158/1078-0432.CCR-08-2362.
GIL-BAZO I CASTANON ALVAREZ E LOPEZ I 1634pinhibitor of differentiation-1 (id1) and id3 expression correlates with epithelial-mesenchymal transition (emt)-related proteins (emtrp) in non-small cell lung carcinoma (nsclc) Ann Oncol 2014 25 4 iv565 iv566 10.1093/annonc/mdu359.6 GIL-BAZO I, CASTANON ALVAREZ E, LOPEZ I, et al . 1634pinhibitor of differentiation-1 (id1) and id3 expression correlates with epithelial-mesenchymal transition (emt)-related proteins (emtrp) in non-small cell lung carcinoma (nsclc)[J ] . Ann Oncol, 2014, 25(4): iv565-iv566. DOI: 10.1093/annonc/mdu359.6.
LI Y WEN X LIU Y Tubular cell dedifferentiation and peritubular inflammation are coupled by the transcription regulator Id1 in renal fibrogenesis Kidney Int 2012 81 9 880 891 10.1038/ki.2011.469 LI Y, WEN X, LIU Y. Tubular cell dedifferentiation and peritubular inflammation are coupled by the transcription regulator Id1 in renal fibrogenesis[J]. Kidney Int, 2012, 81(9): 880-891. DOI: 10.1038/ki.2011.469.
QIU J PENG Q ZHENG Y OxLDL stimulates Id1 nucleocytoplasmic shuttling in endothelial cell angiogenesis via PI3K pathway Biochim Biophys Acta 2012 1821 10 1361 1369 10.1016/j.bbalip.2012.07.016 QIU J, PENG Q, ZHENG Y, et al . OxLDL stimulates Id1 nucleocytoplasmic shuttling in endothelial cell angiogenesis via PI3K pathway[J ] . Biochim Biophys Acta, 2012, 1821(10): 1361-1369. DOI: 10.1016/j.bbalip.2012.07.016.
LEE M J KEE K H SUH C H Capsaicin-induced apoptosis is regulated by endoplasmic reticulum stress- and calpain-mediated mitochondrial cell death pathways Toxicology 2009 264 3 205 214 10.1016/j.tox.2009.08.012 LEE M J, KEE K H, SUH C H, et al . Capsaicin-induced apoptosis is regulated by endoplasmic reticulum stress- and calpain-mediated mitochondrial cell death pathways[J ] . Toxicology, 2009, 264(3): 205-214. DOI: 10.1016/j.tox.2009.08.012.
HONG Z F ZHAO W X YIN Z Y Capsaicin enhances the drug sensitivity of cholangiocarcinoma through the inhibition of chemotherapeutic-induced autophagy PLoS ONE 2015 10 5 e0121538 10.1371/journal.pone.0121538 HONG Z F, ZHAO W X, YIN Z Y, et al . Capsaicin enhances the drug sensitivity of cholangiocarcinoma through the inhibition of chemotherapeutic-induced autophagy[J ] . PLoS ONE, 2015, 10(5): e0121538. DOI: 10.1371/journal.pone.0121538.
DERRY S RICE A S COLE P Topical capsaicin (high concentration) for chronic neuropathic pain in adults Cochrane Database Syst Rev 2017 1 CD007393 10.1002/14651858.CD007393.pub4 DERRY S, RICE A S, COLE P, et al . Topical capsaicin (high concentration) for chronic neuropathic pain in adults[J ] . Cochrane Database Syst Rev, 2017, 1: CD007393. DOI: 10.1002/14651858.CD007393.pub4.
PENG X WANG Y KOLLI S Physical and functional interaction between the ID1 and p65 for activation of NF-κB Am J Physiol Cell Physiol 2012 303 3 C267 C277 10.1152/ajpcell.00365.2011 PENG X, WANG Y, KOLLI S, et al . Physical and functional interaction between the ID1 and p65 for activation of NF-κB[J ] . Am J Physiol Cell Physiol, 2012, 303(3): C267-C277. DOI: 10.1152/ajpcell.00365.2011.
KAMAID A NEVES J GIRALDEZ F Id gene regulation and function in the prosensory domains of the chicken inner ear: a link between Bmp signaling and Atoh1 J Neurosci 2010 30 34 11426 11434 10.1523/JNEUROSCI.2570-10.2010 KAMAID A, NEVES J, GIRALDEZ F. Id gene regulation and function in the prosensory domains of the chicken inner ear: a link between Bmp signaling and Atoh1[J]. J Neurosci, 2010, 30(34): 11426-11434. DOI: 10.1523/JNEUROSCI.2570-10.2010.
0
浏览量
0
下载量
0
CSCD
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
