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低频低强度超声对巨噬细胞内耻垢分枝杆菌的损伤效应
Low-frequency and low-intensity ultrasound suppresses activity of
Mycobacterium smegmatis in macrophages- 第三军医大学学报 2018年40卷第16期 页码：1476-1482
- 作者机构：
- 作者简介：
- 基金信息：重庆市基础科学与前沿技术研究专项一般项目(CSCT2016jcyjA0098);重庆市社会事业与民生保障专项一般项目(CSCT2016shmszx130029)
DOI：10.16016/j.1000-5404.201802075
中图分类号：收稿：2018-02-15，
纸质出版：2018-08-30
- 稿件说明：
移动端阅览
谢霜, 杨敏, 杜永洪, 等. 低频低强度超声对巨噬细胞内耻垢分枝杆菌的损伤效应[J]. 第三军医大学学报, 2018,40(16):1476-1482.
Shuang XIE, Min YANG, Yonghong DU, et al. Low-frequency and low-intensity ultrasound suppresses activity of
Mycobacterium smegmatis in macrophages[J]. Journal of Third Military Medical University, 2018, 40(16): 1476-1482.谢霜, 杨敏, 杜永洪, 等. 低频低强度超声对巨噬细胞内耻垢分枝杆菌的损伤效应[J]. 第三军医大学学报, 2018,40(16):1476-1482. DOI： 10.16016/j.1000-5404.201802075.
Shuang XIE, Min YANG, Yonghong DU, et al. Low-frequency and low-intensity ultrasound suppresses activity of
Mycobacterium smegmatis in macrophages[J]. Journal of Third Military Medical University, 2018, 40(16): 1476-1482. DOI： 10.16016/j.1000-5404.201802075.
摘要
Abstract
Objective
2
To investigate the effect of low-frequency and low-intensity ultrasound (LFLIU) in inducing damages of
Mycobacterium smegmatis
(
M.smegmatis
) in macrophages and explore the mechanism.
Methods
2
Murine macrophage RAW264.7 cells were exposed to ultrasound irradiation for 5
10 or 15 min
and the changes in the cell viability were analyzed with MTT assay.The apoptotic and necrosis rates of RAW264.7 cells after ultrasonic irradiation and after phagocytosis of
M.smegmatis
were assessed using flow cytometry; the activity of
M.smegmatis
in RAW264.7 cells following the exposure was evaluated with plate colony counting.
Laser scanning confocal microscopy was used to observe the production of reactive oxygen species (ROS) in RAW264.7 cells after ultrasound exposure.
Results
2
Exposure of RAW264.7 cells to a ultrasonic intensity of 0.14 W/cm
2
for 5 min and 10 min resulted in cell survival rates of (116.37±9.54)% and (97.14±9.26)%
respectively
suggesting no significant impact of LFLIU exposure on the cell viability.The apoptotic rate of RAW264.7 cells increased significantly after irradiation with 0.14 W/cm
2
ultrasound for 5 min compared with the control cells (
P
<
0.05).The results of plate colony counting suggested that the viability of
M.smegmatis
in RAW264.7 cells was significantly lowered after exposure to a ultrasound intensity of 0.14 W/cm
2
for 5 and 10 min.Confocal laser scanning microscopy revealed obvious ROS production in RAW264.7 cells in response to 0.14 W/cm
2
ultrasound exposure.
Conclusion
2
Exposure to LFLIU induces apoptosis and ROS production in RAW264.7 cells and causes decreased activity of
M.smegmatis
within the cells.
关键词
Keywords
references
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