先天性肛门直肠畸形患儿血清VA及肠组织RARα表达及意义

王至立; 谷成超; 汪泉; 孙静; 侯金平; 刘伟; 李晓庆; 毕杨; 王佚

doi:10.16016/j.1000-5404.201802071

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先天性肛门直肠畸形患儿血清VA及肠组织RARα表达及意义

临床医学 | 更新时间：2026-01-09

- 先天性肛门直肠畸形患儿血清VA及肠组织RARα表达及意义
- Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation
- 第三军医大学学报 2018年40卷第17期页码：1584-1589
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家临床重点专科建设项目(国卫办医函〔2013〕544);重庆市卫计委医学科研重点项目(20141010)
- DOI：10.16016/j.1000-5404.201802071
  中图分类号：
- 收稿：2018-02-09，
  
  纸质出版：2018-09-15
- 稿件说明：
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王至立, 谷成超, 汪泉, 等. 先天性肛门直肠畸形患儿血清VA及肠组织RARα表达及意义[J]. 第三军医大学学报, 2018,40(17):1584-1589.

Zhili WANG, Chengchao GU, Quan WANG, et al. Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation[J]. Journal of Third Military Medical University, 2018, 40(17): 1584-1589.
王至立, 谷成超, 汪泉, 等. 先天性肛门直肠畸形患儿血清VA及肠组织RARα表达及意义[J]. 第三军医大学学报, 2018,40(17):1584-1589. DOI： 10.16016/j.1000-5404.201802071.

Zhili WANG, Chengchao GU, Quan WANG, et al. Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation[J]. Journal of Third Military Medical University, 2018, 40(17): 1584-1589. DOI： 10.16016/j.1000-5404.201802071.

摘要

Abstract

Objective

To investigate the serum content of vitamin A (VA) and the intestinal expression of retinoic acid receptor α (RARα) in the children with congenital anorectal malformation (ARM)

and explore the possible association of them with abnormalities of ARM enteric nervous system (ENS).

Methods

A total of 32 freshly-diagnosed ARM neonates who undergoing elective staged surgical treatment in our hospital from February 2015 to February 2017 were recruited in this study. High performance liquid chromatography was used to detect the serum VA content in the 32 ARM neonates and 30 neonates (as control) without alimentary tract malformations. RT-PCR was employed to detect the mRNA expression of RARα in intestinal specimens at the end of rectum and stoma from 30 ARM neonates and 4 neonates (as control) without alimentary tract malformations. According to the results of pathological study on the intestinal tissues at the end of rectum

the tissues were divided into normal and abnormal ganglion cells groups (

=6 for each group). Then immunohistochemical assay and Western blotting were adopted to detect the expression of RARα protein in the rectum specimens.

Results

The serum content of VA was significantly lower in the ARM neonates than the control neonates (0.40±0.14

0.54±0.10 μmol/L

0.01). The expression level of RARα mRNA was significantly lower in the rectum of ARM (0.78±0.40) than in the

colon of stoma (1.28±0.72

0.01) and the rectum of control (1.12±0.14

0.01)

but there was no difference in the level between the colon of stoma and rectum of control (

0.05). RARα were expressed in the nucleus of ganglion cells and nerve fibers in the rectum. The protein level of RARα was significantly lower in the abnormal ganglion cells group than the normal ganglion cells group (0.77±0.08

1.03±0.06

0.01).

Conclusion

ARM neonates have lower serum VA content and abnormal intestinal expression of RARα.

关键词

Keywords

references

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