早期类风湿关节炎女性患者阴道微生态菌群基因组分析

惠彩霞; 张娟; 曾本华; 钟兵; 万萍; 杨莉; 吴力克; 魏泓; 方勇飞

doi:10.16016/j.1000-5404.201802065

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早期类风湿关节炎女性患者阴道微生态菌群基因组分析

临床医学 | 更新时间：2026-01-09

- 早期类风湿关节炎女性患者阴道微生态菌群基因组分析
- Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients
- 第三军医大学学报 2018年40卷第11期页码：1005-1010
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81072921)
- DOI：10.16016/j.1000-5404.201802065
  中图分类号：
- 收稿：2018-02-07，
  
  纸质出版：2018-06-15
- 稿件说明：
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惠彩霞, 张娟, 曾本华, 等. 早期类风湿关节炎女性患者阴道微生态菌群基因组分析[J]. 第三军医大学学报, 2018,40(11):1005-1010.

Caixia HUI, Juan ZHANG, Benhua ZENG, et al. Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients[J]. Journal of Third Military Medical University, 2018, 40(11): 1005-1010.
惠彩霞, 张娟, 曾本华, 等. 早期类风湿关节炎女性患者阴道微生态菌群基因组分析[J]. 第三军医大学学报, 2018,40(11):1005-1010. DOI： 10.16016/j.1000-5404.201802065.

Caixia HUI, Juan ZHANG, Benhua ZENG, et al. Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients[J]. Journal of Third Military Medical University, 2018, 40(11): 1005-1010. DOI： 10.16016/j.1000-5404.201802065.

摘要

Abstract

Objective

To analyze the differences of vaginal microbiome between the patients with early rheumatoid arthritis (RA) and healthy reproductive-age women from the overall perspective of bacteria.

Methods

A total of 24 vaginal swabs were collected from 12 early RA patients and 12 healthy women (control). The bacterial genomic DNA of all obtained samples was extracted for amplifying the 16S rRNA V3 and V4 hypervariable region by PCR and pyrosequencing by Illumina. The abundance and composition of vaginal microbiota were analyzed by Uparse and LEfSe software.

Results

The Alpha diversity was significantly increased in the bacterial strains from the early RA patients (P=0.04). Lactobacillus was the predominant genus in healthy women (accounting for 84.98%). The amount of Lactobacillus was decreased (11.01%) but that of Gardnerella increased (43.16%) significantly in the RA group. Moreover

the relative abundances of Bifidobacterium (9.94%)

Streptococcus (8.05%) and Atopobium (7.86%) were increased in different degrees. The results of principal coordinate analysis (PCoA) and hierarchical cluster analysis showed that the structure of vaginal flora in RA group had high similarity

and the vaginal microflora structure of the healthy control group could be distinctly distinguished. LEfSe analysis found that there were signific

ant differences in the abundance of Lactobacillus

Gardiner

bifidobacteria and Singularities at the genus level (

=0.017

0.046

0.018

0.007).

Conclusion

A correlation is found between vaginal dysbacteriosis and RA. The diversity of vaginal microbiome is increased in the RA patients

with significantly decreased relative abundance of Lactobacillus

while increased abundance of Gardnerella.

关键词

Keywords

references

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