肌动蛋白调节分子CAP1对肺癌细胞迁移和增殖的影响

李炯; 周颦; 白平; 刘楠; 张国铎; 徐健众; 苏立

doi:10.16016/j.1000-5404.201802037

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肌动蛋白调节分子CAP1对肺癌细胞迁移和增殖的影响

临床医学 | 更新时间：2026-01-09

- 肌动蛋白调节分子CAP1对肺癌细胞迁移和增殖的影响
- Adenylatecyclase-associated protein 1 enhances motility and promotes proliferation of non-small cell lung cancer cells in vitro
- 第三军医大学学报 2018年40卷第16期页码：1500-1506
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市卫计委医学科研重点项目(2013-1-037)
- DOI：10.16016/j.1000-5404.201802037
  中图分类号：
- 收稿：2018-02-06，
  
  纸质出版：2018-08-30
- 稿件说明：
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李炯, 周颦, 白平, 等. 肌动蛋白调节分子CAP1对肺癌细胞迁移和增殖的影响[J]. 第三军医大学学报, 2018,40(16):1500-1506.

Jiong LI, Pin ZHOU, Ping BAI, et al. Adenylatecyclase-associated protein 1 enhances motility and promotes proliferation of non-small cell lung cancer cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1500-1506.
李炯, 周颦, 白平, 等. 肌动蛋白调节分子CAP1对肺癌细胞迁移和增殖的影响[J]. 第三军医大学学报, 2018,40(16):1500-1506. DOI： 10.16016/j.1000-5404.201802037.

Jiong LI, Pin ZHOU, Ping BAI, et al. Adenylatecyclase-associated protein 1 enhances motility and promotes proliferation of non-small cell lung cancer cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1500-1506. DOI： 10.16016/j.1000-5404.201802037.

摘要

Abstract

Objective

To investigate the expression of adenylatecyclase-associated protein 1 (CAP1) in human non-small cell lung cancer (NSCLC) and its role in the migration and proliferation of NSCLC cells.

Methods

Immunohistochemistry was used to detect the expression of CAP1 protein in clinical specimens of NSCLC

adjacent tissues and normal lung tissues from 42 patients.Two human NSCLC cell lines (A549 and H460) with CAP1 knockdown by shRNA lentivirus infection were examined for changes in cell motility using scratch wound healing test and Transwell assay

and the cell proliferation was assessed using MTS test and colony formation assay; and changes of cytoskeleton were observed with immunofluorescence assay and the cell cycle changes were analyzed using flow cytometry.

Results

The clinical specimens of NSCLC showed obvious CAP1 overexpression as compared with the adjacent tissues and normal lung tissues (

0.01).Knockdown of CAP1 significantly impaired the migration (

0.01) and proliferation of NSCLC cells

in vitro

resulting also in depressed clone formation ability (

0.01)

obvious changes in actin cytoskeleton

and cell cycle arrest in G

phase.

Conclusion

CAP1 promotes the motility and proliferation of NSCLC cells possibly by regulating actin cytoskeleton and cell cycle.

关键词

Keywords

references

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