低强度聚焦超声通过瞬时受体电位M7促进骨髓间充质干细胞成骨分化

姚欢; 郑妍; 李娅莎; 赵丽; 杨珂

doi:10.16016/j.1000-5404.201802006

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低强度聚焦超声通过瞬时受体电位M7促进骨髓间充质干细胞成骨分化

基础医学 | 更新时间：2026-01-09

- 低强度聚焦超声通过瞬时受体电位M7促进骨髓间充质干细胞成骨分化
- Low-intensity focused ultrasound promotes mouse mesenchymal stem cell osteogenesis via transient receptor potential melastatin 7
- 第三军医大学学报 2018年40卷第16期页码：1469-1475
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市自然科学基金一般项目(CSTC2017jcyjAX0171);重庆市自然科学基金一般项目(CSTC2014jcyjA10090)
- DOI：10.16016/j.1000-5404.201802006
  中图分类号：
- 收稿：2018-02-02，
  
  纸质出版：2018-08-30
- 稿件说明：
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姚欢, 郑妍, 李娅莎, 等. 低强度聚焦超声通过瞬时受体电位M7促进骨髓间充质干细胞成骨分化[J]. 第三军医大学学报, 2018,40(16):1469-1475.

Huan YAO, Yan ZHENG, Yasha LI, et al. Low-intensity focused ultrasound promotes mouse mesenchymal stem cell osteogenesis via transient receptor potential melastatin 7[J]. Journal of Third Military Medical University, 2018, 40(16): 1469-1475.
姚欢, 郑妍, 李娅莎, 等. 低强度聚焦超声通过瞬时受体电位M7促进骨髓间充质干细胞成骨分化[J]. 第三军医大学学报, 2018,40(16):1469-1475. DOI： 10.16016/j.1000-5404.201802006.

Huan YAO, Yan ZHENG, Yasha LI, et al. Low-intensity focused ultrasound promotes mouse mesenchymal stem cell osteogenesis via transient receptor potential melastatin 7[J]. Journal of Third Military Medical University, 2018, 40(16): 1469-1475. DOI： 10.16016/j.1000-5404.201802006.

摘要

Abstract

Objective

To explore the role of transient receptor potential melastatin 7 (Trpm7) in mediating the effect of low-intensity pulsed ultrasound (LIPUS) to promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).

Methods

Cultured mouse BMSCs were identified for specific surface markers using flow cytometry.LIPUS treatment time was optimized according to alkaline phosphatase (ALP) readings.The osteogenic differentiation of the cells after LIPUS treatment was observed using ALP and alizarin red staining

and the mRNA levels of Runx2

OPN

OCN and Trpm7 at 24

48 and 72 h following the treatment were detected using qPCR.The effects of LIPUS in the presence of DMSO or 2-APB were tested on ALP activity and osteogenic differentiation of the cells; Western blotting was used to detect the protein expression levels of Trpm7

OPN and Runx2.

Results

The BMSCs showed the most distinct increase of ALP reading following LIPUS treatment for 2 min

which resulted in significantly enhanced ALP and alizarin red staining and increased mRNA levels of OPN

OCN and Runx2 (

0.05).Trpm7 mRNA expression increased significantly in the cells at 24

48 and 72 h following LIPUS treatment (

0.05).ALP activity was significantly lower in the cells with LIPUS+2-APB treatment than in those treated with LIPUS alone or with LIPUS+DMSO (

0.05).The cells treated with LIPUS and LIPUS+DMSO exhibited obvious enhancement of ALP staining

alizarin red staining and protein expressions of Trpm7

OPN and Runx2

and these changes were significantly suppressed in cells treated with LIPUS and 2-APB.

Conclusion

LIPUS promotes osteogenic differentiation of BMSCs

in vitro

partly through Trpm7.

关键词

Keywords

references

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