阿司匹林对小鼠胶质瘤干细胞增殖及凋亡的体外研究

邹德伟; 王俊伟; 汪攀; 廖彬; 潘金玉; 李成仁; 吴南

doi:10.16016/j.1000-5404.201801221

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阿司匹林对小鼠胶质瘤干细胞增殖及凋亡的体外研究

基础医学 | 更新时间：2026-01-09

- 阿司匹林对小鼠胶质瘤干细胞增殖及凋亡的体外研究
- Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro
- 第三军医大学学报 2018年40卷第16期页码：1456-1460
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81672493);西南医院院管课题(SWH2016JCYB-42)
- DOI：10.16016/j.1000-5404.201801221
  中图分类号：
- 收稿：2018-01-25，
  
  纸质出版：2018-08-30
- 稿件说明：
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邹德伟, 王俊伟, 汪攀, 等. 阿司匹林对小鼠胶质瘤干细胞增殖及凋亡的体外研究[J]. 第三军医大学学报, 2018,40(16):1456-1460.

Dewei ZOU, Junwei WANG, Pan WANG, et al. Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1456-1460.
邹德伟, 王俊伟, 汪攀, 等. 阿司匹林对小鼠胶质瘤干细胞增殖及凋亡的体外研究[J]. 第三军医大学学报, 2018,40(16):1456-1460. DOI： 10.16016/j.1000-5404.201801221.

Dewei ZOU, Junwei WANG, Pan WANG, et al. Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1456-1460. DOI： 10.16016/j.1000-5404.201801221.

摘要

Abstract

Objective

To study the effects of aspirin on proliferation and apoptosis of mouse glioma stem cells (GSCs).

Methods

In vitro

cultured GSCs isolated from mouse glioma cell line GL261 were treated with 2.5

5.0 and 10 mmol/L aspirin

with normal saline as the control.The pro

liferation of GSCs was assayed using CCK-8 kit

and the changes in cell cycle distribution and cell apoptosis were detected with flow cytometry and TUNEL assay

respectively; LDH kit was used to assess the cell damage after aspirin treatment.

Results

Compared with the control cells

the GSCs treated with different concentrations of aspirin showed dose- and time-dependent inhibition of cell proliferation (

0.05) and obviously reduced number and size of the tumor spheres (

0.05).Aspirin caused obvious cell cycle changes in GSCs

shown by a significantly decreased percentage of S phase cells and an increased percentage of G

phase cells (

0.05).Aspirin also induced obvious apoptosis of GSCs and significantly increased the release of cytosolic LDH (

0.05).

Conclusion

Aspirin inhibits the proliferation and promotes apoptosis of mouse GSCs

in vitro

关键词

Keywords

references

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