超声造影动态评价兔类风湿关节炎模型炎症活动性的价值

吴长洁; 王亚辉; 华兴; 郭燕丽; 滕永亮; 刘雪婷

doi:10.16016/j.1000-5404.201801218

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超声造影动态评价兔类风湿关节炎模型炎症活动性的价值

基础医学 | 更新时间：2026-01-09

- 超声造影动态评价兔类风湿关节炎模型炎症活动性的价值
- Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound
- 第三军医大学学报 2018年40卷第11期页码：984-990
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81471677)
- DOI：10.16016/j.1000-5404.201801218
  中图分类号：
- 收稿：2018-01-26，
  
  纸质出版：2018-06
- 稿件说明：
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吴长洁, 王亚辉, 华兴, 等. 超声造影动态评价兔类风湿关节炎模型炎症活动性的价值[J]. 第三军医大学学报, 2018,40(11):984-990.

Changjie WU, Yahui WANG, Xing HUA, et al. Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound[J]. Journal of Third Military Medical University, 2018, 40(11): 984-990.
吴长洁, 王亚辉, 华兴, 等. 超声造影动态评价兔类风湿关节炎模型炎症活动性的价值[J]. 第三军医大学学报, 2018,40(11):984-990. DOI： 10.16016/j.1000-5404.201801218.

Changjie WU, Yahui WANG, Xing HUA, et al. Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound[J]. Journal of Third Military Medical University, 2018, 40(11): 984-990. DOI： 10.16016/j.1000-5404.201801218.

摘要

Abstract

Objective

To evaluate the value of contrast-enhanced ultrasound (CEUS) in the dynamic detection of inflammation activity in rabbit model of rheumatoid arthritis (RA).

Methods

A total of 24 rabbits (both sexes

6 months old

weighing 3.0~3.5 kg) were used to establish RA model

via

ovalbumin sensitization in 48 knee joints. The rabbits were divided into 4 groups

that is

control (pre-modeling)

M1 (1 week post induction)

M4 (4 weeks post induction) and M8 (8 weeks post induction) groups

with 12 knees in each group. Ultrasound examination including grey scale ultrasonography and power Doppler imaging (PDI) was performed in both femorotibial joints of each rabbit to measure the synovial thickness and semi-quantitatively score (0~3). CEUS was subsequently performed to evaluate the enhancement of synovium. Time-intensity curve (TIC) was drawn with automatic fitting

and the data including peak intensity (PI)

peak time (PT)

area under curve (AUC) and half time of descending (T1/2) were determined automatically. All the rats were killed

and the synovial tissues were collected for pathological examination. The synovial vascular bed area index was calculated in each group

and the results were compared. The correlations between ultrasonic data and pathologic data were analyzed.

Results

Ultrasonographic results showed that the thickness of femorotibial synovium was increased with modeling time

with significant differences among groups (

0.01). There was a significant difference in PDI score between the M4 and M8 groups (

0.05). A positive correlation was found between synovial thickness and pathologic score (

=0.887

0.01). Positive rate of CEUS was higher than that of PDI in both the M4 and M8 groups (8/12

3/12

12/12

10/12). Among the TIC data

the values of PI and AUC in the synovial tissues were lower than those of surrounding fat tissues (

0.01). There were positive correlations of the PI value

AUC value and PDI score of the synovial tissue with synovial vascular bed area index (

=0.664

0.01;

=0.711

0.01;

=0.483

0.05).

Conclusion

Grey scale ultrasonography can reflect the pathological changes of synovitis. CEUS is more sensitive than PDI in reflecting the formation of early angiogenesis in the synovium

and is valuable in the early dynamic detection of inflammation activity in RA.

关键词

Keywords

references

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