miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响

刘方杰; 何晓燕; 谭正兰; 邹琳

doi:10.16016/j.1000-5404.201801190

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miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响

基础医学 | 更新时间：2026-01-09

- miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响
- Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice
- 第三军医大学学报 2018年40卷第15期页码：1370-1374
- 作者机构：
  
  400014 重庆, 重庆医科大学附属儿童医院临床分子医学中心, 儿童发育疾病研究教育部重点实验室, 儿科学重庆市重点实验室, 重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地
- 作者简介：
  
  [ 邹琳, 电话:(023)63622128, E-mail:zoulin74@126.com ZOU Lin, E-mail:zoulin74@126.com
- 基金信息：
  
  国家自然科学基金青年科学基金(81201543)
- DOI：10.16016/j.1000-5404.201801190
  中图分类号： R394.3;R730.23;R730.264
- 收稿：2018-01-23，
  
  修回：2018-02-08，
  
  纸质出版：2018-08-15
- 稿件说明：
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刘方杰, 何晓燕, 谭正兰, 等. miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响[J]. 第三军医大学学报, 2018,40(15):1370-1374.

Fangjie LIU, Xiaoyan HE, Zhenglan TAN, et al. Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice[J]. Journal of Third Military Medical University, 2018, 40(15): 1370-1374.
刘方杰, 何晓燕, 谭正兰, 等. miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响[J]. 第三军医大学学报, 2018,40(15):1370-1374. DOI： 10.16016/j.1000-5404.201801190.

Fangjie LIU, Xiaoyan HE, Zhenglan TAN, et al. Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice[J]. Journal of Third Military Medical University, 2018, 40(15): 1370-1374. DOI： 10.16016/j.1000-5404.201801190.

摘要

目的

研究高表达miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响及其可能的分子机制。

方法

将18只裸鼠按照随机区组法分成3组：SH-SY5Y组（空白对照组）、miR-221-up组（实验组）和miRNA-NC组（阴性对照组），分别接种SH-SY5Y细胞、稳定转染miR-221的SH-SY5Y-miR-221细胞及稳定转染对照质粒的SH-SY5Y-miRNA-NC细胞，观察裸鼠体内成瘤及生长情况，30 d后处死取瘤体测量肿瘤体积、质量；qRT-PCR检测各组肿瘤组织中miR-221和MYCN mRNA水平的表达；Western blot和免疫组织化学法检测肿瘤组织中MYCN蛋白的表达水平。

结果

与SH-SY5Y组相比，miR-221-up组裸鼠的瘤体体积和质量明显增加（

0.01），miRNA-NC组无统计学差异（

0.05）；与SH-SY5Y组相比，miR-221-up组中miR-221、MYCN mRNA以及MYCN蛋白的表达明显升高（

0.01），而miRNA-NC组与SH-SY5Y组相比无统计学差异（

0.05）。

结论

miR-221可能通过上调MYCN的表达，促进SH-SY5Y细胞在裸鼠体内的生长。

Abstract

Objective

To investigate the effect and potential underlying mechanisms of miR-221 on the growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice.

Methods

Eighteen nude mice were randomly divided into SH-SY5Y group (blank control group)

miR-221-up group (SH-SY5Y cells with miR-221 overexpression

experimental group) and miRNA-NC group (SH-SY5Y cells with miR-221 scramble siRNA

negative control group). Then SH-SY5Y cells

and stable transfected cells over-expressing miR-221 and miRNA-NC miRNA were respectively injected subcutaneously in the back of nude mice. The growth of tumor masses was observed. In 30 d after cell transplantation

the mice were sacrificed

and the tumor masses were taken for the weight and volume. Then the mRNA expression levels of miR-221 and MYCN in the tumor masses were analyzed by qRT-PCR. The protein expression of MYCN was measured by Western blotting and immunohistochemical assay.

Results

Significant increases in tumor size and weight were observed in the tumor masses of the miR-221-up group than those of the SH-SY5Y group (

0.05)

but no such differences were seen when compared with those of the miRNA-NC group (

0.05). The expression levels of miR-221 and MYCN mRNAs and MYCN protein were markedly increased in miR-221-up group than the SH-SY5Y group (

0.01)

but there were no such differences in the above expression levels between the SH-SY5Y group and miRNA-NC group (

0.05).

Conclusion

MiR-221 could promote the growth of SH-SY5Y neuroblastoma cells xenografts through enhancing MYCN expression.

关键词

Keywords

references

BURCHILL S A, BEISKE K, SHIMADA H, et al . Recommendations for the standardization of bone marrow disease assessment and reporting in children with neuroblastoma on behalf of the International Neuroblastoma Response Criteria Bone Marrow Working Group[J ] .Cancer, 2017, 123(7):1095-1105. DOI:10.1002/cncr.30380.

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