生物蛋白胶和曲安奈德对犬食管内镜黏膜下剥离术后狭窄的预防

贾益君; 吴宏博; 王凌; 覃语思; 彭贵勇

doi:10.16016/j.1000-5404.201801131

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生物蛋白胶和曲安奈德对犬食管内镜黏膜下剥离术后狭窄的预防

基础医学 | 更新时间：2026-01-09

- 生物蛋白胶和曲安奈德对犬食管内镜黏膜下剥离术后狭窄的预防
- Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model
- 第三军医大学学报 2018年40卷第11期页码：979-983
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81070384);国家自然科学基金面上项目(81470907);第三军医大学第一附属医院重大领域技术创新项目(SWH2016ZDCX2011)
- DOI：10.16016/j.1000-5404.201801131
  中图分类号：
- 收稿：2018-01-02，
  
  纸质出版：2018-06
- 稿件说明：
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贾益君, 吴宏博, 王凌, 等. 生物蛋白胶和曲安奈德对犬食管内镜黏膜下剥离术后狭窄的预防[J]. 第三军医大学学报, 2018,40(11):979-983.

Yijun JIA, Hongbo WU, Ling WANG, et al. Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model[J]. Journal of Third Military Medical University, 2018, 40(11): 979-983.
贾益君, 吴宏博, 王凌, 等. 生物蛋白胶和曲安奈德对犬食管内镜黏膜下剥离术后狭窄的预防[J]. 第三军医大学学报, 2018,40(11):979-983. DOI： 10.16016/j.1000-5404.201801131.

Yijun JIA, Hongbo WU, Ling WANG, et al. Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model[J]. Journal of Third Military Medical University, 2018, 40(11): 979-983. DOI： 10.16016/j.1000-5404.201801131.

摘要

Abstract

Objective

To investigate the preventive efficacy of fibrin glue supplementation with triamcinolone acetonide in a dog model of post-endoscopic esophageal dissection (ESD).

Methods

Twenty 12-month-old domestic dogs in good condition (both sexes

12 to 15 kg)

were randomly divided into 4 groups (

=5):fibrin glue-triamcinolone acetonide treatment group (FT)

fibrin glue treatment group (F)

triamcinolone acetonide treatment group (T) and control group. ESD model was inflicted by exfoliating three-quarters circumference of esophageal mucosa in 30~34 cm away from the incisor. Then fibrin glue suspensions with triamcinolone acetonide

fibrin glue solution or triamcinolone acetonide solution were correspondingly sprayed to cover the ulcer wound after ESD

respectively

and no drug was applied for the control group. Gross observation

stenosis rate

and HE staining and immunohistochemical examination of α-SMA for the stenosis tissues were performed in 6 weeks after ESD.

Results

Mucosal folds were sighted under endoscopy but in which a thinner esophageal wall was noted in macropathology. The dogs in the FT group gained a lower mucosal stenosis rate as to the dogs in the T group (

0.05) and control group (

0.01)

and a similar lower rate happened in the comparison with the dogs from the F group to the control group (

0.05). HE staining displayed atrophy in the muscularis propria

extensive myofibroblasts and collagen fibers in submucosa layer in the control group. Compared with the FT group

the expression level of α-SMA was augmented in the T group and control group (

0.01)

as well as in the T group (

0.01) and control group (

0.05) when compared with the F group.

Conclusion

Spraying fibrin glue-triamcinolone acetonide suspensions or fibrin glue alone can relieve esophageal post-ESD stenosis

probably

via

alleviating submucosa fibrosis and inflammation

but a single dose of triamcinolone acetonide does not work.

关键词

Keywords

references

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