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lncRNA-TUG1促进放射后人骨髓间充质干细胞衰老
Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells
in vitro - 第三军医大学学报 2018年40卷第10期 页码：904-908
- 作者机构：
400037 重庆, 陆军军医大学(第三军医大学)第二附属医院输血科
- 作者简介：
[ 李忠俊, E-mail: zhongjunli@tmmu.edu.cn LI Zhongjun, E-mail: zhongjunli@tmmu.edu.cn
- 基金信息：
DOI：10.16016/j.1000-5404.201801017
中图分类号： R329.2; R818.023; R818.74收稿：2018-01-03，
修回：2018-03-13，
纸质出版：2018-05-30
- 稿件说明：
移动端阅览
吴江, 张薇薇, 李忠俊. lncRNA-TUG1促进放射后人骨髓间充质干细胞衰老[J]. 第三军医大学学报, 2018,40(10):904-908.
Jiang WU, Weiwei ZHANG, Zhongjun LI. Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells
in vitro [J]. Journal of Third Military Medical University, 2018, 40(10): 904-908.吴江, 张薇薇, 李忠俊. lncRNA-TUG1促进放射后人骨髓间充质干细胞衰老[J]. 第三军医大学学报, 2018,40(10):904-908. DOI： 10.16016/j.1000-5404.201801017.
Jiang WU, Weiwei ZHANG, Zhongjun LI. Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells
in vitro [J]. Journal of Third Military Medical University, 2018, 40(10): 904-908. DOI： 10.16016/j.1000-5404.201801017.
摘要
目的
2
探讨lncRNA-TUG1在放射导致的骨髓间充质干细胞(bone marrow mesenchymal stem cells
BM-MSCs)衰老中所起的作用。
方法
2
实验分为lncRNA-TUG1干扰组、lncRNA-TUG1对照组，用γ射线造成放射损伤后，采用实时荧光定量PCR(RT-PCR)检测lncRNA-TUG1在2组的表达变化，采用Western blot检测2组的γ-H2AX蛋白反映DNA损伤，检测衰老相关蛋白p53表达以及β-半乳糖苷酶染色反映细胞衰老。
结果
2
γ射线放射显著增加了BM-MSCs中lncRNA-TUG1表达(
P
<
0.01)，BM-MSCs中迅速出现γ-H2AX磷酸化，p53蛋白水平升高，BM-MSCs衰老明显增加；而干扰lncRNA-TUG1后，放射后BM-MSCs的DNA损伤修复加快，p53蛋白水平下降，细胞衰老明显减少(
P
<
0.01)。
结论
2
放射可能通过lncRNA-TUG1促进BM-MSCs的衰老。
Abstract
Objective
2
To study the role of long noncoding RNA TUG1 (LncRNA-TUG1) in γ radiation-induced senescence of human bone marrow mesenchymal stem cells (BM-MSCs)
in vitro
.
Methods
2
Human BM-MSCs were transfected with a lentiviral vector carrying short-hairpin RNA (shRNA) targeting LncRNA-TUG1 (LncRNA-TUG1-SI) or with an empty lentiviral vector prior to γ-ray radiation. Cell senescence following the exposure was analyzed using SA-β-Gal staining
and Western blotting was used to detect the changes in the expressions of γ-H2AX (an indicator of DNA double-strand breaks) and p53 in the cells.
Results
2
γ-ray radiation at 9 Gy resulted in rapid γ-H2AX phosphorylation
significantly increased cell senescence
and increased protein levels enhanced expression of p53 and the expression of LncRNA-TUG1 in human BM-MSCs. In contrast
BM-MSCs transfected with LncRNA-TUG1 shRNA showed a fast recovery from γ radiation-induced DNA damage with significantly reduced cell senescence and decreased protein levels of p53.
Conclusions
2
LncRNA-TUG1 may promote senescence of BM-MSCs following γ radiation and can serve as a potential target for intervention of radiation-induced senescence of BM-MSCs.
关键词
Keywords
references
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