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成纤维细胞生长因子受体2对小肠上皮细胞增殖与分化的影响
Effect of fibroblast growth factor receptor 2 on proliferation and differentiation of intestinal epithelial cells in adult mice
- 第三军医大学学报 2018年40卷第10期 页码：847-854
- 作者机构：
1.400038 重庆，陆军军医大学(第三军医大学)：军事预防医学系全军复合伤研究所
2.400038 重庆，陆军军医大学(第三军医大学)：第三附属医院(野战外科研究所)创伤实验室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
[粟永萍, E-mail: suyp2003@163.com SU Yongping, E-mail: suyp2003@163.com
- 基金信息：国家自然科学基金面上项目(81472916);全军医学科研“十二五”计划重大项目(AWS13J002);“十二五”全军后勤科研项目重大专项(AWS14C002)
DOI：10.16016/j.1000-5404.201801006
中图分类号： R322.45; R329.28; R394.2收稿：2018-01-02，
修回：2018-01-23，
纸质出版：2018-05-30
- 稿件说明：
移动端阅览
唐才智, 任泂, 高美娇, 等. 成纤维细胞生长因子受体2对小肠上皮细胞增殖与分化的影响[J]. 第三军医大学学报, 2018,40(10):847-854.
Caizhi TANG, Jiong REN, Meijiao GAO, et al. Effect of fibroblast growth factor receptor 2 on proliferation and differentiation of intestinal epithelial cells in adult mice[J]. Journal of Third Military Medical University, 2018, 40(10): 847-854.
唐才智, 任泂, 高美娇, 等. 成纤维细胞生长因子受体2对小肠上皮细胞增殖与分化的影响[J]. 第三军医大学学报, 2018,40(10):847-854. DOI： 10.16016/j.1000-5404.201801006.
Caizhi TANG, Jiong REN, Meijiao GAO, et al. Effect of fibroblast growth factor receptor 2 on proliferation and differentiation of intestinal epithelial cells in adult mice[J]. Journal of Third Military Medical University, 2018, 40(10): 847-854. DOI： 10.16016/j.1000-5404.201801006.
摘要
目的
2
探究成纤维细胞生长因子受体2(fibroblast growth factor receptor 2，Fgfr2)功能缺失对成年小鼠小肠上皮细胞增殖和分化的影响。
方法
2
繁殖和培育小肠上皮Fgfr2条件性敲除小鼠，经他莫昔芬(tamoxifen)诱导，Western blot验证Fgfr2在肠上皮的特异性基因敲除情况；将诱导肠上皮敲除Fgfr2的小鼠作为实验组，同胎野生型小鼠作为对照组。利用免疫荧光技术对潘氏细胞和内分泌细胞占肠上皮细胞的比例进行计数，利用Alcian染色对杯状细胞占肠上皮细胞的比例进行计数；运用HE染色和免疫组化染色指标推断小肠上皮Fgfr2功能缺失对小肠上皮细胞增殖的影响。
结果
2
经过转基因小鼠繁殖后诱导，Western blot检测结果显示小肠上皮特定敲除的Fgfr2小鼠构建成功；免疫荧光和Alcian染色结果显示：与对照组小鼠比较，Fgfr2敲除小鼠的十二指肠、空肠和回肠上皮潘氏细胞数量增加(
P
<
0.05)，杯状细胞数量减少(
P
<
0.05)，而内分泌细胞数量不变(
P
>
0.05)；HE染色和免疫组化染色结果显示：绒毛长度和隐窝深度以及增殖细胞数目未见明显变化(
P
>
0.05)。
结论
2
Fgfr2介导的微环境信号可参与调控成年小鼠小肠上皮分泌系细胞分化，但对小肠上皮细胞增殖无显著影响。
Abstract
Objective
2
To investigate the effect of functional loss of fibroblast growth factor receptor 2 (Fgfr2) on the proliferation and differentiation of small intestinal epithelial cells in adult mice.
Methods
2
Transgenic mice with conditional knockout of Fgfr2 gene in the small intestinal epithelium were bred and the offspring mice were identified for genotypes and tamoxifen-induced Fgfr2 gene knockout in the small intestinal epithelium. The small intestinal secretory cells were characterized by Alcian staining and immunofluorescence staining to analyze the proportions of different intestinal secretory cells in the epithelium. HE staining and immunohistochemistry were used to observe the length of the villus and crypt depth and quantify the proliferating cells in the crypt in Fgfr2-deficient mice and wild-type littermate mice.
Results
2
After tamoxifen induction
the transgenic mice showed specific Fgfr2 knockout in the small intestinal epithelium as verified by Western blotting. Immunofluorescence and Alcian staining revealed significantly increased Paneth cells (
P
<
0.05) and decreased goblet cells (
P
<
0.05) in the small intestine epithelium of Fgfr2-deficient mice as compared with the wild-type littermate mice
but the number of small intestinal endocrine cells exhibited no significant changes in the transgenic mice. Immunohistochemistry and HE staining showed no significant changes in villus length
crypt depth or the ratio of proliferating cells in the crypt in Fgfr2-deficient mice as compared with the wild-type mice (all
P
>
0.05).
Conclusion
2
Fgfr2-mediated microenvironment signals affect the differentiation of small intestine epithelial cells in adult mice but have little effect on the proliferation of small intestinal epithelial cells.
关键词
Keywords
references
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