高盐对小鼠白色脂肪棕色化的影响及其中枢调控机制研究

张晨亮; 林毅漳; 周鑫; 陈霞; 林树; 宋治远

doi:10.16016/j.1000-5404.201712261

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高盐对小鼠白色脂肪棕色化的影响及其中枢调控机制研究

基础医学 | 更新时间：2026-01-09

- 高盐对小鼠白色脂肪棕色化的影响及其中枢调控机制研究
- Effect of high salt intake on browning of white adipose tissue in mice and mechanism of its central regulation
- 第三军医大学学报 2018年40卷第10期页码：868-874
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院心血管内科，重庆市介入心脏病学研究所
- 作者简介：
  
  [宋治远, E-mail: zysong2010@126.com SONG Zhiyuan, E-mail: zysong2010@126.com
- 基金信息：
  
  国家自然科学基金面上项目(81570395);重庆市西南医院院管课题(SWH2016HWHZ-04)
- DOI：10.16016/j.1000-5404.201712261
  中图分类号： R151.2;R349.15;R363.22
- 收稿：2017-12-15，
  
  修回：2018-03-15，
  
  纸质出版：2018-05-30
- 稿件说明：
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张晨亮, 林毅漳, 周鑫, 等. 高盐对小鼠白色脂肪棕色化的影响及其中枢调控机制研究[J]. 第三军医大学学报, 2018,40(10):868-874.

Chenliang ZHANG, Yizhang LIN, Xin ZHOU, et al. Effect of high salt intake on browning of white adipose tissue in mice and mechanism of its central regulation[J]. Journal of Third Military Medical University, 2018, 40(10): 868-874.
张晨亮, 林毅漳, 周鑫, 等. 高盐对小鼠白色脂肪棕色化的影响及其中枢调控机制研究[J]. 第三军医大学学报, 2018,40(10):868-874. DOI： 10.16016/j.1000-5404.201712261.

Chenliang ZHANG, Yizhang LIN, Xin ZHOU, et al. Effect of high salt intake on browning of white adipose tissue in mice and mechanism of its central regulation[J]. Journal of Third Military Medical University, 2018, 40(10): 868-874. DOI： 10.16016/j.1000-5404.201712261.

摘要

目的

研究高盐摄入对小鼠白色脂肪组织(white adipose tissue，WAT)棕色化的影响，探讨其可能的中枢调控机制。

方法

采用随机数字表法将20只8周龄的C57BL/6J小鼠分为4组(

=5)：短期高盐组与短期对照组(饲养24 h后处死，免疫组化法检测小鼠大脑c-Fos表达情况)，长期高盐组与长期对照组(连续饲养8周，每周对体质量、体温、饮水、进食进行监测)。对照组正常饮水，高盐组给予含2%氯化钠的盐水。长期组实验结束后收集血清检测血脂水平。收集性腺脂肪和脑标本，用荧光定量PCR法检测性腺脂肪组织中解耦联蛋白-1(uncoupling protein-1，UCP-1)等棕色化相关调控基因的改变以及下丘脑室旁核脑源性生长因子(brain derived neurotrophic factor，BDNF)的mRNA表达。

结果

短期高盐刺激后，小鼠下丘脑室旁核c-Fos表达高盐组（112.67±11.24）较对照组（30.67±5.51）明显增加（

0.01）。给予小鼠8周的高盐刺激后，小鼠血中甘油三酯[（0.75±0.19）mmol/L

（1.07±0.08）mmol/L，

0.05

]

和游离脂肪酸含量下降[（0.64±0.14）mmol/L

（0.92±0.15）mmol/L，

0.05

]

，单侧

性腺脂肪质量明显减轻[（0.11±0.01）g

（0.15±0.01）g，

0.01

]

。荧光定量PCR法检测发现高盐组WAT棕色化标志基因UCP-1含量（4.62±0.94）较对照组（1.00±0.16）明显增加（

0.01）；下丘脑室旁核BDNF高盐组mRNA表达（3.14±0.31）较对照组（1.00±0.12）明显增加（

0.01）。

结论

长期高盐可诱导小鼠WAT棕色化，而下丘脑室旁核BDNF参与了其中的中枢调控过程。

Abstract

Objective

To investigate the effect of high salt intake on the browning of white adipose tissue (WAT) in mice and explore the central regulatory mechanism underlying this effect.

Methods

Twenty male C57BL/6J mice were randomly allocated into control and high salt intake groups and were given free access to normal water and 2% NaCl solution

respectively. Each group was further divided into 2 subgroups for observation at 24 h and at 8 weeks. The mice in 24 h groups were sacrificed for immunohistochemistry of c-Fos expression in the brain at 24 h. In 8 weeks groups

the mice were examined for body weight

body temperature

water intake and food intake on a weekly basis for 8 weeks

after which the mice were sacrificed for measurement of blood lipid levels and detection of mRNA expression of uncoupling protein-1 (UCP-1) in epididymal WAT and brain derived neurotrophic factor (BDNF) in the paraventricular nucleus (PVN) using qRT-PCR.

Results

The mice with high salt intake for 24 h showed significantly increased number of c-Fos-positive neurons in the PVN compared with the corresponding control group (112.67±11.24

30.67±5.51

0.01). Compared with the control group

high-salt intake for 8 weeks caused a significant decrease in the weight of WAT (0.11±0.01

0.15±0.01 g

0.01)

content of TG (0.75±0.19

1.07±0.08 mmol/L

0.05) and levels of free fatty acids (0.64±0.14

0.92±

0.15 mmol/L

0.05)

and significantly increased the transcriptional level of UCP-1 in the WAT (4.62±0.94

1.00±0.16

0.01) and the mRNA expression of BDNF in the PVN (3.14±0.31

1.00±0.12

0.01).

Conclusion

Long-term high salt intake can induce the browning of WAT

and BDNF in the PVN participates in the central regulation of this process.

关键词

Keywords

references

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