长链非编码RNA LINC01140对急性髓系白血病U937细胞增殖及周期的影响

骆青松; 胡晓烨; 朱垚; 高宁

doi:10.16016/j.1000-5404.201712217

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长链非编码RNA LINC01140对急性髓系白血病U937细胞增殖及周期的影响

基础医学 | 更新时间：2026-01-09

- 长链非编码RNA LINC01140对急性髓系白血病U937细胞增殖及周期的影响
- Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells
- 第三军医大学学报 2018年40卷第9期页码：768-773
- 作者机构：
  
  400038 重庆, 陆军军医大学(第三军医大学)药学与检验医学系生药学教研室
- 作者简介：
  
  [高宁, E-mail: gaoning59@163.com GAO Ning, E-mail:gaoning59@163.com
- 基金信息：
  
  国家自然科学基金面上项目(31571425)
- DOI：10.16016/j.1000-5404.201712217
  中图分类号： R394.3;R730.23;R733.71
- 收稿：2017-12-22，
  
  修回：2018-02-22，
  
  纸质出版：2018-05-15
- 稿件说明：
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骆青松, 胡晓烨, 朱垚, 等. 长链非编码RNA LINC01140对急性髓系白血病U937细胞增殖及周期的影响[J]. 第三军医大学学报, 2018,40(9):768-773.

Qingsong LUO, Xiaoye HU, Yao ZHU, et al. Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells[J]. Journal of Third Military Medical University, 2018, 40(9): 768-773.
骆青松, 胡晓烨, 朱垚, 等. 长链非编码RNA LINC01140对急性髓系白血病U937细胞增殖及周期的影响[J]. 第三军医大学学报, 2018,40(9):768-773. DOI： 10.16016/j.1000-5404.201712217.

Qingsong LUO, Xiaoye HU, Yao ZHU, et al. Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells[J]. Journal of Third Military Medical University, 2018, 40(9): 768-773. DOI： 10.16016/j.1000-5404.201712217.

摘要

目的

探讨长链非编码RNA (long non-coding RNA

LncRNA) LINC01140对U937细胞增殖及周期的影响。

方法

收集陆军军医大学第一附属医院血液科2016年7-12月20例急性髓细胞白血病(acute myeloid leukemia

AML)患者骨髓标本和2017年5-6月在血液科进行骨髓检查提示为正常的10例骨髓标本

采用qRT-PCR检测AML患者骨髓及白血病细胞中LINC01140的表达情况。通过构建LncRNA LINC01140-shRNA慢病毒干扰载体转染U937细胞(对照组为shR-NC

实验组为shR-1、shR-2)

采用qRT-PCR检测LINC01140的表达。细胞计数法、细胞克隆成球实验分别检测干扰LINC01140表达对U937细胞增殖和克隆成球能力的影响。流式细胞术、蛋白免疫印迹分别检测U937细胞周期和周期相关蛋白的变化。

结果

① LncRNA LINC01140在白血病患者和细胞中表达显著升高(

0.01)。②下调LINC01140表达

U937细胞增殖减慢

细胞克隆成球数量减少

表明细胞增殖能力显著降低(

0.01)。③流式细胞术检测结果显示干扰LINC01140表达可导致U937细胞G

期阻滞

其中shR-1和shR-2的G

期细胞的百分比分别为(62.89±1.66)%、(59.03±1.00)%

相对于对照组的(46.25±1.06)%

差异有统计学意义(

0.01)。Western blot检测结果显示干扰LINC01140表达可下调U937细胞中G

期相关蛋白Cyclin E1的表达

并可升高G

期相关蛋白p21和p27的表达。

结论

LncRNA LINC01140在AML患者中高表达

在U937细胞中敲降LINC01140可导致细胞周期G

期阻滞

从而显著抑制细胞增殖。

Abstract

Objective

To investigate the effect of long non-coding RNA (LncRNA) LINC01140 on proliferation and cell cycle in U937 cells.

Methods

Bone marrow samples from 20 acute myeloid leukemia (AML) patients identified in the hematological department of our university from July to December 2016

and from 10 healthy individuals for examination during May and June 2017 were collected in this study.Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LINC01140 in the bone marrow tissues and leukemia cells.Lentiviral vector LncRNA LINC01140 was constructed

and then transfected into U937 cells (shR-NC in the control group

shR-1 and shR-2 in the experimental group).The expression of LINC01140 was detected by qRT-PCR.Cell proliferation and colony formation were detected by cell counting and tumor sphere formation assay.Cell cycle distribution and the expression of related proteins were determined by flow cytometry and Western blot analyses

respectively.

Results

① LncRNA LINC01140 was highly expressed in the bone marrow tissues from AML patients and U937 cells (

0.01).② Knockdown of LINC01140 with siRNA significantly inhibited cell proliferation and colony formation in U937 cells (

0.01).③ Flow cytometry indicated that knockdown of LINC01140 resulted in cell cycle arrested at G

phase

with the proportion of G

phase cells accounting for (62.89±1.66)% and (59.03±1.00)% respectively in shR-1 and shR-2 cells

which were significantly higher than that in the control cells [(46.25± 1.06)%

0.01

]

.Western blotting showed that the knockdown of LINC01140 decreased the expression level of G

phase related protein Cyclin E1 and increased those of p21 and p27.

Conclusion

LncRNA LINC01140 is highly expressed in AML patients.Its knockdown induces cell cycle arrested in G

phase and thus leads inhibition of cell proliferation in U937 cells.

关键词

Keywords

references

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