多重耐药铜绿假单胞菌的两种同源性分析方法的比较

李进; 胡韦维; 张峰领; 黎敏; 陈鸣; 邓少丽; 鲁卫平

doi:10.16016/j.1000-5404.201712024

您当前的位置：

首页 >

文章列表页 >

多重耐药铜绿假单胞菌的两种同源性分析方法的比较

基础医学 | 更新时间：2026-01-09

- 多重耐药铜绿假单胞菌的两种同源性分析方法的比较
- Comparison of MALDI-TOF MS and PFGE for homology analysis of clinical isolates of multi-drug resistant Pseudomonas aeruginosa
- 第三军医大学学报 2018年40卷第14期页码：1258-1262
- 作者机构：
  
  1.重庆 400042，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)检验科
  2.重庆 400014，重庆医科大学附属儿童医院检验科
- 作者简介：
  
  [鲁卫平，E-mail: ronnylu@126.com LU Weiping, E-mail: ronnylu@126.com
- 基金信息：
  
  国家自然科学基金重点项目(81430053);全军后勤计划重大专项课题子课题(AWS14C003-2)
- DOI：10.16016/j.1000-5404.201712024
  中图分类号： R372; R378.911
- 收稿：2017-12-04，
  
  修回：2018-04-12，
  
  纸质出版：2018-07-30
- 稿件说明：
移动端阅览
李进, 胡韦维, 张峰领, 等. 多重耐药铜绿假单胞菌的两种同源性分析方法的比较[J]. 第三军医大学学报, 2018,40(14):1258-1262.

Jin LI, Weiwei HU, Fengling ZHANG, et al. Comparison of MALDI-TOF MS and PFGE for homology analysis of clinical isolates of multi-drug resistant Pseudomonas aeruginosa[J]. Journal of Third Military Medical University, 2018, 40(14): 1258-1262.
李进, 胡韦维, 张峰领, 等. 多重耐药铜绿假单胞菌的两种同源性分析方法的比较[J]. 第三军医大学学报, 2018,40(14):1258-1262. DOI： 10.16016/j.1000-5404.201712024.

Jin LI, Weiwei HU, Fengling ZHANG, et al. Comparison of MALDI-TOF MS and PFGE for homology analysis of clinical isolates of multi-drug resistant Pseudomonas aeruginosa[J]. Journal of Third Military Medical University, 2018, 40(14): 1258-1262. DOI： 10.16016/j.1000-5404.201712024.

摘要

目的

以脉冲场凝胶电泳(pulsed field gel electrophoresis

PFGE)为金标准，评价基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry，MALDI-TOF MS)在多重耐药铜绿假单胞菌(multi-drug resistant

Pseudomonas aeruginosa

，MDR-PA)同源性分析中应用的可行性。

方法

收集陆军军医大学第三附属医院2017年1-12月重症监护病房住院患者分离的20株MDR-PA非重复菌株，利用VITEK MS SARAMIS软件对MDR-PA蛋白图谱进行聚类分析，同时对其进行PFGE同源性分析，并将两种方法所得的分型结果进行对比分析。

结果

MALDI-TOF MS同源性分析结果与PFGE同源性分析结果比较显示，20株MDR-PA通过MALDI-TOF MS聚类分析将其分为三大簇(Ⅰ型13株，Ⅱ型6株，Ⅲ型1株)，而依据PFGE分型结果则将其分为6种亚型(A型2株，B型3株，C型4株，D型3株，E型7株，F型1株)，其中有C型和E型中具有相同基因型菌株间，其MALDI-TOF MS亲缘性关系也较近，而其余4种亚型菌株间亲缘性比较，发现PFGE分型结果与MALDI-TOF MS聚类分析结果不完全相符。

结论

与PFGE同源性分析相比，MALDI-TOF MS同源性分析结果一致性不稳定，具有一定局限性，尚不能取代PFGE同源性分析。

Abstract

Objective

To assess the feasibility of using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for homology analysis of multi-drug resistant

Pseudomonas aeruginosa

(MDR-PA) with pulsed-field gel electrophoresis (PFGE) as the gold standard.

Methods

Twenty clinical isolates of MDR-PA were collected from the intensive care unit (ICU) of our Third Affiliated Hospital in the year 2017. SARAMIS software was used for cluster analysis of the isolates

and their homology was analyzed using PFGE and MALDI-TOF MS.

Results

Homology analysis by MALDI-TOF MS grouped the 20 MDR-PA strains into 3 clusters (13 type Ⅰ strains

6 type Ⅱ strains

and 1 type Ⅲ strain). According to PFGE classification results

these strains were divided into 6 subtypes

namely type A (2 strains)

type B (3 strains)

type C (4 strains)

type D (3 strains)

type E (7 strains)

and type F (1 strain). The type C and type E strains classified by PFGE were consistent with the results of cluster analysis by MALDI-TOF MS

but the results of PFGE classification and MALDI-TOF MS cluster analysis showed discrepancies in homology analysis of the strains of the other 4 subtypes.

Conclusion

Compared with PEGE

MALDI-TOF MS has certain unstability and limitations in homology analysis of clinical isolates of MDR-PA

and still can't replace the other.

关键词

Keywords

references

袁莉莉, 丁百兴, 沈震, 等.耐碳青霉烯类铜绿假单胞菌的耐药性及分子流行病学研究[J].中国感染与化疗杂志, 2017, 17(3): 289-292.

YUAN L L, DING B X, SHEN Z, et al. Antibiotic resistance and molecular epidemiology of carbapenem-resistant Pseudomonas aeruginosa [J]. Chin J Infec Chemoth, 2017, 17(3): 289-292.

黄小荣, 梁天文, 刘配辰, 等.多重耐药铜绿假单胞菌同源性分析及Ⅰ型整合子研究[J].中国抗生素杂志, 2014, 39(10): 764-768.

HUANG X R, LIANG T W, LIU P C, et al. Homological analysis of multi-drug resistant Pseudomonas aeruginosa and detection of class Ⅰ integrons [J]. Chin J Antibio, 2014, 39(10): 764-768.

WATERS V, ZLOSNIK J E, YAU Y C, et al. Comparison of three typing methods for Pseudomonas aeruginosa isolates from patients with cystic fibrosis[J]. Eur J Clin Microbiol Infect Dis, 2012, 31(12): 3341-3350. DOI:10.1007/s10096-012-1701-z.

SUZUKI M, YAMADA K, AOKI M, et al. Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa[J]. J Appl Microbiol, 2016, 120(2): 487-497. DOI: 10.1111/jam.13016.

BADER O. MALDI-TOF-MS-based species identification and typing approaches in medical mycology[J]. Proteomics, 2013, 13(5): 788-799. DOI:10.1002/pmic.201200468.

MENCACCI A, MONARI C, LELI C, et al. Typing of nosocomial outbreaks of Acinetobacter baumannii by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol, 2013, 51(2): 603-606. DOI: 10.1128/JCM.01811-12.

李东菊, 朱元祺, 梁冰. MALDI-TOF MS用于肺炎克雷伯菌同源性分析的初步研究[J].中国微生态学杂志, 2016, 28(5): 528-532+537.

LI D J, ZHU Y Q, LIANG B. MALDI-TOF homology analysis of Klebsiella pneumoniae by MALDI-TOF MS [J]. Chin J Microecol, 2016, 28(5): 528-532+537.

王亚南, 高晶晶, 钟桥, 等. MALDI-TOF MS技术在鲍曼不动杆菌鉴定及同源性分析中的应用[J].临床检验杂志, 2015, 33(6): 430-433.

WANG Y N, GAO J J, ZHONG Q, et al. MALDI-TOF identification and homology analysis of Acinetobacter baumannii by use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J]. Chin J Clin Lab Sci, 2015, 33(6): 430-433.

THONG K L, LAI K S, GANESWRIE R, et al. Pulsed-field gel electrophoresis of multidrug-resistant and -sensitive strains of Pseudomonas aeruginosa from a Malaysian hospital[J]. Jpn J Infect Dis, 2004, 57(5): 206-209.

VERROKEN A, BAURAING C, DEPLANO A, et al. Epidemiological investigation of anosocomial outbreak of multidrug-resistant Corynebacterium striatum at one Belgian university hospital[J]. Clin Microbiol Infect, 2014, 20(1): 44-50. DOI:10.1111/1469-0691.12197.

闻海丰, 冯忠军, 秦瑾, 等.采用ERIC-PCR与PFGE分析鲍曼不动杆菌基因型和同源性并对比方法学差异[J].分子诊断与治疗杂志, 2016, 8(1): 27-31.

WEN H F, FENG Z J, QIN J, et al. Comparison between PFGE and ERIC-PCR for genotyping and homologous analysis of Acinetobacter baumannii [J]. J Mol Diagn Ther, 2016, 8(1): 27-31.

杨富国, 闫志勇, 毕春霞.青岛地区医院内感染耐甲氧西林金黄色葡萄球菌分子分型研究[J].中华流行病学杂志, 2008, 29(12): 1230-1234.

YANG F G, YAN Z Y, BI C X. Molecular typing of methieillin-resistant Staphylococcus aureus isolated from hospitalized patients in Qingdao [J]. Chin J Epidemiol, 2008, 29(12): 1230-1234.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于分子分型监测的大肠埃希菌耐药性分析与公共卫生防控策略

广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析