棘突形态及功能重塑在癫痫形成中的研究

袁靳闲; 刘熙; 欧书; 许韬; 陈阳美

doi:10.16016/j.1000-5404.201711208

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棘突形态及功能重塑在癫痫形成中的研究

基础医学 | 更新时间：2026-01-09

- 棘突形态及功能重塑在癫痫形成中的研究
- Changes of dendritic spine morphology and functions in the development of epilepsy in rats
- 第三军医大学学报 2018年40卷第8期页码：686-692
- 作者机构：
  
  400016 重庆，重庆医科大学附属第二医院神经内科
- 作者简介：
  
  [陈阳美，E-mail:489455407@qq.comCHEN Yangmei, E-mail:489455407@qq.com
- 基金信息：
  
  国家自然科学基金面上项目(81571259)
- DOI：10.16016/j.1000-5404.201711208
  中图分类号： R338.13;R363.21;R742.1
- 收稿：2017-11-24，
  
  修回：2018-01-15，
  
  纸质出版：2018-04-30
- 稿件说明：
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袁靳闲, 刘熙, 欧书, 等. 棘突形态及功能重塑在癫痫形成中的研究[J]. 第三军医大学学报, 2018,40(8):686-692.

Jinxian YUAN, Xi LIU, Shu OU, et al. Changes of dendritic spine morphology and functions in the development of epilepsy in rats[J]. Journal of Third Military Medical University, 2018, 40(8): 686-692.
袁靳闲, 刘熙, 欧书, 等. 棘突形态及功能重塑在癫痫形成中的研究[J]. 第三军医大学学报, 2018,40(8):686-692. DOI： 10.16016/j.1000-5404.201711208.

Jinxian YUAN, Xi LIU, Shu OU, et al. Changes of dendritic spine morphology and functions in the development of epilepsy in rats[J]. Journal of Third Military Medical University, 2018, 40(8): 686-692. DOI： 10.16016/j.1000-5404.201711208.

摘要

目的

研究癫痫形成过程中棘突形态、功能变化以及棘突重塑在癫痫形成过程中的可能作用。

方法

① 20只SD大鼠随机数字表法分为癫痫组及对照组，建立锂-匹罗卡品慢性癫痫模型，高尔基染色观察大鼠海马齿状回棘突形态变化，qRT-PCR及Western blot检测海马PSD95及SYP表达变化。②选择SD胎鼠海马神经元进行原代培养，建立无镁癫痫模型。检测细胞内钙离子浓度及神经元棘突数量变化情况统计两者相关性。

结果

① 与对照组相比，癫痫模型组大鼠齿状回(dentate gyrus

DG)区棘突平均密度增加，但正常功能棘突数量减少，PSD95及SYP表达增加(

0.01)②癫痫组细胞内钙离子浓度增加(

0.01)，且与神经元棘突密度变化呈正相关(

=0.613，

0.05)。

结论

癫痫形成过程中突触重塑活跃。但形态及功能异常，为癫痫形成提供基础，可作为棘突重塑的重要标志。

Abstract

Objective

To investigate the morphological and functional changes of dendritic spines during the development of epilepsy and explore the possible role of dendritic spine remodeling in epilepsy.

Methods

Twenty SD rats were randomly divided into control group and epilepsy group

and in the latter group

rat models of chronic epilepsy were established by intraperitoneal injection of lithium and pilocarpine. The morphology of the dendritic spines of the neurons in the hippocampal dentate gyrus (DG) was observed using Golgi staining

and the changes in the expression levels of postsynaptic density protein 95 (PSD95) and synaptophysin (SYP) were detected using qRT-PCR and Western blotting. Hippocampal neurons were isolated from fetal SD rats for primary culture

and an epileptic cell model was established using magnesium-free medium. The changes in intracellular calcium concentration and the density of dendritic spines were assessed in the cells after modeling.

Results

Compared with the control rats

the epileptic rats showed significantly increased mean density of dendritic spines in the DG neurons (

0.05) with reduced mushroom-shaped spines (

0.05); The expression of PSD95 and SYP in the hippocampus was significantly increased in the epileptic rats (

0.05). In the hippocampal neuronal model of epilepsy

intracellular calcium concentration increased significantly after modeling compared with that in the control cells (

0.01) and showed a significant positive correlation with the density of dendritic spines in the neurons (

0.05).

Conclusion

Synaptic remodeling is super-active during epilepsy with morphological and functional abnormalities of the synapses

which provides the pathological basis for epilepsy and serves as an important sign of dendritic spine remodeling.

关键词

Keywords

references

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