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MiR-223通过靶向PARP1对氧化应激状态下人主动脉内皮细胞HAECs存活的影响
Overexpression of miR-223 reduces survival of human aortic endothelial cells with H2O2-induced oxidative stress
in vitro by directly targeting PARP1- 第三军医大学学报 2018年40卷第14期 页码：1301-1305
- 作者机构：
473000 河南南阳，南阳医学高等专科学校第一附属医院神经内科
- 作者简介：
[马金玉，E-mail：574810234@qq.com MA Jinyu, E-mail:574810234@qq.com
- 基金信息：
DOI：10.16016/j.1000-5404.201711201
中图分类号： R322.121; R363.22; R394-3收稿：2017-11-23，
修回：2018-04-21，
纸质出版：2018-07-30
- 稿件说明：
移动端阅览
马金玉, 江康, 冯涛. MiR-223通过靶向PARP1对氧化应激状态下人主动脉内皮细胞HAECs存活的影响[J]. 第三军医大学学报, 2018,40(14):1301-1305.
Jinyu MA, Kang JIANG, Tao FENG. Overexpression of miR-223 reduces survival of human aortic endothelial cells with H2O2-induced oxidative stress
in vitro by directly targeting PARP1[J]. Journal of Third Military Medical University, 2018, 40(14): 1301-1305.马金玉, 江康, 冯涛. MiR-223通过靶向PARP1对氧化应激状态下人主动脉内皮细胞HAECs存活的影响[J]. 第三军医大学学报, 2018,40(14):1301-1305. DOI： 10.16016/j.1000-5404.201711201.
Jinyu MA, Kang JIANG, Tao FENG. Overexpression of miR-223 reduces survival of human aortic endothelial cells with H2O2-induced oxidative stress
in vitro by directly targeting PARP1[J]. Journal of Third Military Medical University, 2018, 40(14): 1301-1305. DOI： 10.16016/j.1000-5404.201711201.
摘要
目的
2
探讨miR-223通过靶向PARP1对氧化应激状态下人主动脉内皮细胞HAECs存活的影响及miR-223改善氧化应激来治疗高血压的潜能。
方法
2
TargetScan预测靶向PARP1的潜在miRNAs；双荧光素酶报告实验检测miR-223对PARP1的靶向调节作用；qPCR和western blot检测miR-223对HAECs细胞中PARP1 mRNA和蛋白表达影响；用H
2
O
2
诱导人主动脉内皮细胞HAECs产生氧化应激，单细胞凝胶电泳、克隆形成实验和MTT分别检测miR-223对细胞DNA损伤、存活能力的影响。
结果
2
软件预测发现miR-223是PARP1的潜在miRNAs；双荧光素酶报告实验、qPCR及western blot均证实了miR-223对PARP1的靶向调控作用；过表达miR-223可通过降低PARP1表达促进H
2
O
2
诱导人主动脉内皮细胞HAECs氧化损伤，降低细胞存活能力。
结论
2
miR-223通过靶向调节PARP1表达降低H
2
O
2
诱导后人主动脉内皮细胞HAECs存活，有可能成为高血压治疗的新靶点。
Abstract
Objective
2
To investigate the effect of miR-223 overexpression on the survival of human aortic endothelial cells (HAECs) under oxidative stress and explore the underlying mechanism.
Methods
2
Targetscan was used to predict the potential miRNAs targeting poly-(ADP-ribose) polymerase 1 (PARP1). Dual luciferase reporter assay
qPCR and Western blotting were used to evaluate the effect of miR-223 overexpression on PARP1 in cultured HAECs transfected with miR-223 using Lipofectamine 2000. The effect of miR-223 overexpression on DNA damage and cell viability in HAECs exposed to H
2
O
2
-induced oxidative stress was investigated using single cell gel electrophoresis
clonogenic assay and MTT assay.
Results
2
Bioinformatics analysis indicated that PARP1 was a potential target of miR-223. The results of dual luciferase reporter assay
qPCR and Western blotting all supported the role of miR-223 for targeted modulation of PARP1 in HAECs. Overexpression of miR-223
obviously exacerbated oxidative cell damage and decreased the cell viability in HAECs exposed to H
2
O
2
.
Conclusion
2
The overexpression of miR-223 reduces the survival of cultured HAECs with H
2
O
2
-induced oxidative stress by targeting PARP1
suggesting the potential of miR-223 as a therapeutic target for improving hypertension induced by oxidative stress.
关键词
Keywords
references
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