双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭

刘洪伟; 梅基雄

doi:10.16016/j.1000-5404.201710089

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双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭

基础医学 | 更新时间：2026-01-09

- 双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭
- Dihydroartemisinin inhibits proliferation and invasion in human gastric cancer cells through suppression of Wnt/β-catenin signaling pathways
- 第三军医大学学报 2018年40卷第5期页码：387-394
- 作者机构：
  
  400021 重庆，重庆市中医院药剂科
- 作者简介：
  
  [梅基雄，E-mail: mjx0025@163.comMEI Jixiong, E-mail: mjx0025@163.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201710089
  中图分类号： R284.1;R735.2;R966
- 收稿：2017-10-15，
  
  修回：2017-12-05，
  
  纸质出版：2018-03-15
- 稿件说明：
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刘洪伟, 梅基雄. 双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭[J]. 第三军医大学学报, 2018,40(5):387-394.

Hongwei LIU, Jixiong MEI. Dihydroartemisinin inhibits proliferation and invasion in human gastric cancer cells through suppression of Wnt/β-catenin signaling pathways[J]. Journal of Third Military Medical University, 2018, 40(5): 387-394.
刘洪伟, 梅基雄. 双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭[J]. 第三军医大学学报, 2018,40(5):387-394. DOI： 10.16016/j.1000-5404.201710089.

Hongwei LIU, Jixiong MEI. Dihydroartemisinin inhibits proliferation and invasion in human gastric cancer cells through suppression of Wnt/β-catenin signaling pathways[J]. Journal of Third Military Medical University, 2018, 40(5): 387-394. DOI： 10.16016/j.1000-5404.201710089.

摘要

目的

探讨双氢青蒿素(dihydroartemisinin

DHA)对胃癌细胞增殖、侵袭和迁移能力的影响及其可能的分子机制。

方法

用不同浓度(10、20、40 μmol/L)的DHA分别作用于人胃癌BGC-823和SGC-7901细胞，采用MTT法、划痕实验、Transwell法和流式细胞术分别检测细胞的增殖能力、迁移能力、侵袭能力和细胞周期与凋亡情况。Western blot检测细胞中DVL2、GSK-3β、p-GSK-3β、β-catenin和Cyclin D1等蛋白的表达量。

结果

不同浓度的DHA可以抑制胃癌细胞的增殖、迁移和侵袭，且呈浓度依赖性增长(

0.05)。DHA作用48 h后，细胞S期比例显著增加，诱导细胞凋亡(

0.05)。DHA可以降低胃癌细胞Dvl2、p-GSK-3β、β-catenin和Cyclin D1的蛋白表达水平(

0.05)，增加GSK-3β蛋白的表达(

0.05)。同时，SKL2001激活Wnt/β-catenin信号通路，逆转DHA对胃癌细胞迁移和侵袭的抑制作用(

0.05)；XAV939抑制Wnt/β-catenin信号通路，进一步加强DHA对胃癌细胞迁移和侵袭的抑制作用(

0.05)。

结论

DHA能有效抑制胃癌细胞的增殖、侵袭和迁移，阻滞细胞周期停留在S期，诱导胃癌细胞凋亡，其机制可能与抑制Wnt/β-catenin信号通路的激活有关。

Abstract

Objective

To investigate the effect of dihydroartemisinin (DHA) on the proliferation and invasion in gastric cancer cells and its possible molecular mechanism.

Methods

Human gastric cancer cell lines BGC-823 and SGC-7901 were exposed to different concentrations of DHA. MTT assay

wound healing assay

Transwell chamber test

and flow cytometry were used respectively to measure the cell proliferation

migration

invasion

and cell cycle distribution and apoptosis. The expression levels of DVL2

GSK-3β

p-GSK-3β

β-catenin and Cyclin D1 were detected by Western blotting.

Results

DHA inhibited the proliferation

invasion and migration of BGC-823 and SGC-7901 cells in a concentration-dependent manner (

0.05). DHA exposure for 48 h significantly increased the percentage of gastric cancer cells at S phase

and induced cell apoptosis (

0.05). DHA treatment resulted in the decreases in the protein levels of DVL2

p-GSK-3β

β-catenin and Cyclin D1 (

0.05)

and increase in that of GSK-3β (

0.05). SKL2001

agonist of the Wnt/β-catenin pathway

reversed the inhibitory effect of DHA on proliferation and invasion of gastric cancer cells (

0.05)

while the inhibitor

XAV939

enhanced the above effects of DHA (

0.05).

Conclusion

DHA inhibits the proliferation

invasion and migration

arrests the cell cycle at S phase and induces cell apoptosis in gastric cancer cells

which may be associated with its suppression of Wnt/β-catenin signaling pathway.

关键词

Keywords

references

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