应用mycalolide B初步建立斑马鱼帕金森病模型

段凌晗; 吴永梅; 李淑蓉; 苏炳银

doi:10.16016/j.1000-5404.201710051

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应用mycalolide B初步建立斑马鱼帕金森病模型

基础医学 | 更新时间：2026-01-09

- 应用mycalolide B初步建立斑马鱼帕金森病模型
- A zebrafish model of Parkinson's disease established using mycalolide B
- 第三军医大学学报 2018年40卷第6期页码：487-493
- 作者机构：
  
  1.610500 成都，成都医学院：人体解剖与组织胚胎学教研室
  2.610500 成都，成都医学院：发育与再生四川省重点实验室
- 作者简介：
  
  [ 苏炳银，E-mail：subingyinn@163.com SU Bingyin, E-mail: subingyinn@163.com
- 基金信息：
  
  国家自然科学基金面上项目(31371215)
- DOI：10.16016/j.1000-5404.201710051
  中图分类号： R-332; R742.5
- 收稿：2017-10-11，
  
  修回：2017-12-18，
  
  纸质出版：2018-03-30
- 稿件说明：
移动端阅览
段凌晗, 吴永梅, 李淑蓉, 等. 应用mycalolide B初步建立斑马鱼帕金森病模型[J]. 第三军医大学学报, 2018,40(6):487-493.

Linghan DUAN, Yongmei WU, Shurong LI, et al. A zebrafish model of Parkinson's disease established using mycalolide B[J]. Journal of Third Military Medical University, 2018, 40(6): 487-493.
段凌晗, 吴永梅, 李淑蓉, 等. 应用mycalolide B初步建立斑马鱼帕金森病模型[J]. 第三军医大学学报, 2018,40(6):487-493. DOI： 10.16016/j.1000-5404.201710051.

Linghan DUAN, Yongmei WU, Shurong LI, et al. A zebrafish model of Parkinson's disease established using mycalolide B[J]. Journal of Third Military Medical University, 2018, 40(6): 487-493. DOI： 10.16016/j.1000-5404.201710051.

摘要

目的

应用mycalolide B(MB)建立斑马鱼帕金森病(Parkison’s disease，PD)模型，观察斑马鱼行为学、形态学及多巴胺神经元的变化并进行系统评价。

方法

同亲代斑马鱼单次产卵200~300枚，简单随机化分为7组：对照组(0.2% DMSO)、MB10组(10 μg/L MB)、MB20组(20 μg/L MB)、MB40组(40 μg/L MB)、MB80组(80 μg/L MB)、MB160组(160 μg/L MB)、1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)组(200 μmol/L MPTP)，每组30枚胚胎，于浓度梯度下培养；统计存活率，分析行为学变化，利用激光共聚焦显微镜观察多巴胺神经元生长情况，并与MPTP组进行比较分析。

结果

MB效应浓度33.4~50.3 μg/L时，成斑马鱼出现发育迟缓、脊椎畸形、心包水肿、平衡失态、震颤等；运动速率降低明显(

＜0.05)；Vmat2-GFP标记的阳性细胞减少(

＜0.01)；synuclein蛋白沉积(

＜0.05)。

结论

建立斑马鱼PD模型的MB效应浓度为33.4~50.3 μg/L；MB诱导的斑马鱼PD模型与MPTP模型相比，行为学、多巴胺神经元数量减少方面相似，且较不产生synuclein的MPTP模型更具模式学意义。

Abstract

Objective

To establish a zebrafish model of Parkinson's disease (PD) using mycalolide B (MB)

and evaluate the model by observing zebrafish behavior

morphology and dopamine neurons.

Methods

A total of 200~300 parental zebrafish eggs from a single spawning were randomly divided into 7 groups: control group

MB10 group (10 μg/L)

MB20 group (20 μg/L)

MB40 group (40 μg/L)

MB80 group (80 μg/L)

MB160 group (160 μg/L) and 1-methyl-4-phenyl-1

6-tetrahydropyridine (MPTP) group (200 μmol/L)

with 30 embryos in each group. The embryos were cultured under the concentration gradient

and the survival rate and behavioral changes were observed. Laser confocal microscopy was used to observe the growth of dopamine neurons. And all the results of MB treatment groups were compared with MPTP group.

Results

The effective concentration of MB was 33.4~50.3 μg/L

which resulted in the abnormities such as growth retardation

spinal deformity

pericardial edema

balance loss and tremor

etc. The movement rate was significantly decreased (

＜0.05). There were less Vmat2-GFP positive cells (

＜0.01) and more synuclein protein deposition (

＜0.05).

Conclusion

MB at an effective concentration of 33.4~50.3 μg/L can be used to establish zebrafish PD model. The zebrafish model induced by MB are similar with the MPTP model in behaviors and fewer dopamine neurons

but better than the other one for synuclein production.

关键词

Keywords

references

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