<sup>131</sup>I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用

季发权; 戚宁; 张东升; 张佳; 张杰; 吉安平; 柏凌; 杜明华; 张龙江

doi:10.16016/j.1000-5404.201709228

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¹³¹I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用

基础医学 | 更新时间：2026-01-09

- ¹³¹I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用
- Inhibitory effect ¹³¹I-antiAFP McAb-DOX-BSA nanoparticles on hepatocellular carcinoma bearing nude mice
- 第三军医大学学报 2018年40卷第5期页码：395-399
- 作者机构：
  
  1.210093 南京，南京大学医学院
  2.210009 南京，东南大学临床医学院
  3.210029 南京，南京中医药大学附属医院/江苏省中医院核医学科
  4.210002 南京，南京大学医学院附属金陵医院/南京军区南京总医院医学影像科
- 作者简介：
  
  [杜明华，E-mail: njdmh@aliyun.comDU Minghua, E-mail: njdmh@aliyun.com
- 基金信息：
  
  国家自然科学基金面上项目(81171452);江苏省自然科学基金面上项目(BK20131455)
- DOI：10.16016/j.1000-5404.201709228
  中图分类号： R735.7;R944.9;R969.4
- 收稿：2017-09-30，
  
  修回：2017-12-07，
  
  纸质出版：2018-03-15
- 稿件说明：
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季发权, 戚宁, 张东升, 等. ¹³¹I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用[J]. 第三军医大学学报, 2018,40(5):395-399.

Faquan JI, Ning QI, Dongsheng ZHANG, et al. Inhibitory effect ¹³¹I-antiAFP McAb-DOX-BSA nanoparticles on hepatocellular carcinoma bearing nude mice[J]. Journal of Third Military Medical University, 2018, 40(5): 395-399.
季发权, 戚宁, 张东升, 等. ¹³¹I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用[J]. 第三军医大学学报, 2018,40(5):395-399. DOI： 10.16016/j.1000-5404.201709228.

Faquan JI, Ning QI, Dongsheng ZHANG, et al. Inhibitory effect ¹³¹I-antiAFP McAb-DOX-BSA nanoparticles on hepatocellular carcinoma bearing nude mice[J]. Journal of Third Military Medical University, 2018, 40(5): 395-399. DOI： 10.16016/j.1000-5404.201709228.

摘要

目的

探讨

131

I-antiAFP McAb-DOX-BSA-NP对肝癌移植瘤的抑制和杀伤作用。

方法

制备

131

I-antiAFP McAb-DOX-BSA-NP；培养人肝癌细胞株BEL-7402及建立荷瘤裸鼠模型；将成瘤裸鼠模型按随机数字表法分为5组，每组6只。A组：空白对照组(生理盐水)；B组：单纯

131

I核素治疗组；C组：

131

I-antiAFP McAb治疗组；D组：

131

I-antiAFP McAb-DOX-BSA-NP治疗组；E组：antiAFP McAb-DOX-BSA-NP治疗组。裸鼠瘤周注射治疗，DOX剂量为40 μg/只，

131

I活度0.2 mCi/只(1 mCi=3.7×10

Bq)，注射体积为0.2 mL/只。于4、9、14、20 d测量并记录各组裸鼠瘤体体积。同时分别在24、48、96、168 h对放射治疗组(B、C、D组)裸鼠进行SPECT检查。

结果

标记产物呈澄清溶液；pH值7.4；放射性化学纯度

99%，活度浓度3.91 mCi/mL；在0.2 mol/L PB缓冲液中1、2、4 h放射性化学纯度分别为97.17%、97.10%、95.22%，稳定性良好。各组裸鼠治疗后肿瘤体积变化及生长抑制率比较：B、C、D、E治疗组较空白对照组肿瘤体积明显减小(

0.05)；与B、C、E

组比较，D组肿瘤生长抑制率最强，差异有统计学意义(

0.05)。SPECT检查结果显示：24 h时，B、C、D 3组瘤区放射性计数值差异无统计学意义(

0.05)；48、96、168 h时，

131

I-antiAFP McAb-DOX-BSA-NP治疗组(D组)及

131

I-antiAFP McAb治疗组(C组)放射性计数值均高于

131

I治疗组(B组)，差异均具有统计学意义(

0.05)，且随时间延长其差异性越来越显著。

结论

单克隆抗体免疫治疗、放射性

131

I内照射治疗及载药纳米粒缓释化疗联合运用对肝癌移植瘤有显著抗癌效果。

Abstract

Objective

To determine the anti-tumor effect of

131

I-antiAFP monoclonal antibody-doxorubicin-bovine serum albumin nanoparticles (

131

I-antiAFP McAb-DOX-BSA-NP) on hepatocarcinoma bearing nude mice.

Methods

The antiAFP McAb-DOX-BSA-NP were prepared and then labelled with radio-iodine. Tumor xenograft model was established by transplanting human hepatoma BEL-7402 cells in nude mice. Then the model mice were randomly divided into 5 groups

that is

blank control group (group A

normal saline)

131

I treatment group (group B)

131

I-antiAFP McAb treatment group (group C)

131

I-antiAFP McAb-DOX-BSA-NP treatment group (group D)

and antiAFP McAb-DOX-BSA-NP treatment group (group E)

with 6 mice in each group. The agents were injected around the tumor mass

with per animal receiving DOX 40 μg and

131

I 0.2 mCi in a total volume of 0.2 mL. The tumor volume was measured in 4

14 and 20 d. The nude mice in groups B

C and D treated with radionuclide were examined with SPECT in 24

96 and 196 h for radioactive counting.

Results

The labeled product presented as clear solution

with a pH value of 7.4

radioactive chemical purity of

99%

and activity of 3.91 mCi/mL. The radioactive chemical purity became 97.17%

97.10% and 95.22% in 1

2 and 4 h respectively

when in the 0.2 mol/L PB buffer

which indicating good stability of the prepared nanoparticles. The tumor size was significant

ly smaller in the groups B

D and E than the group A (

0.05). The tumor growth rate was most strongly suppressed in the group D

with obvious differences when compared with those in groups B

C and E (

0.05). SPECT indicated that there was no statistical difference in the radioactive count in groups B

C and D in 24 h (

0.05)

but the counts in 48

96 and 168 h were notably higher in the groups D and C than the group B (

0.05)

and the differences were more significant over time.

Conclusion

Our prepared nanoparticles show significant anti-tumor effect for hepatocellular carcinoma bearing nude mice.

关键词

Keywords

references

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131I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用

Inhibitory effect 131I-antiAFP McAb-DOX-BSA nanoparticles on hepatocellular carcinoma bearing nude mice

DOI：10.16016/j.1000-5404.201709228

摘要

Abstract

关键词

Keywords

references

¹³¹I-antiAFP导向载药纳米粒对肝癌移植瘤的抑制作用

Inhibitory effect ¹³¹I-antiAFP McAb-DOX-BSA nanoparticles on hepatocellular carcinoma bearing nude mice