FoxM1通过Wnt/β-catenin信号通路对鼻咽癌细胞5-8F增殖、迁移的影响

万婕; 祝琳; 雷越; 刘亚男; 文韬宇; 李丹丹; 叶琳; 陈鸿雁

doi:10.16016/j.1000-5404.201709189

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FoxM1通过Wnt/β-catenin信号通路对鼻咽癌细胞5-8F增殖、迁移的影响

基础医学 | 更新时间：2026-01-09

- FoxM1通过Wnt/β-catenin信号通路对鼻咽癌细胞5-8F增殖、迁移的影响
- Effect of siRNA mediated FoxM1 silencing on proliferation and migration of nasopharyngeal carcinoma 5-8F cells via Wnt/β-catenin signaling pathway
- 第三军医大学学报 2018年40卷第5期页码：380-386, 414
- 作者机构：
  
  1.400016 重庆，重庆医科大学附属第一医院耳鼻喉科
  2.646000 四川泸州，西南医科大学附属医院耳鼻咽喉头颈外科
  3.400016 重庆，重庆医科大学附属第一医院胸心外科
- 作者简介：
  
  [陈鸿雁，E-mail: chenhongyan1963@hotmail.comCHEN Hongyan, E-mail: chenhongyan1963@hotmail.com
- 基金信息：
  
  国家临床重点专科建设项目(〔2012〕649)
- DOI：10.16016/j.1000-5404.201709189
  中图分类号： R394.3;R730.23;R739.63
- 收稿：2017-09-24，
  
  修回：2017-11-06，
  
  纸质出版：2018-03-15
- 稿件说明：
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万婕, 祝琳, 雷越, 等. FoxM1通过Wnt/β-catenin信号通路对鼻咽癌细胞5-8F增殖、迁移的影响[J]. 第三军医大学学报, 2018,40(5):380-386, 414.

Jie WAN, Lin ZHU, Yue LEI, et al. Effect of siRNA mediated FoxM1 silencing on proliferation and migration of nasopharyngeal carcinoma 5-8F cells via Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2018, 40(5): 380-386, 414.
万婕, 祝琳, 雷越, 等. FoxM1通过Wnt/β-catenin信号通路对鼻咽癌细胞5-8F增殖、迁移的影响[J]. 第三军医大学学报, 2018,40(5):380-386, 414. DOI： 10.16016/j.1000-5404.201709189.

Jie WAN, Lin ZHU, Yue LEI, et al. Effect of siRNA mediated FoxM1 silencing on proliferation and migration of nasopharyngeal carcinoma 5-8F cells via Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2018, 40(5): 380-386, 414. DOI： 10.16016/j.1000-5404.201709189.

摘要

目的

探讨FoxM1对鼻咽癌细胞5-8F恶性生物学行为的影响及其可能机制。

方法

将化学合成的靶向FoxM1的siRNA转染鼻咽癌细胞5-8F(FoxM1-siRNA组)，并设立空白对照组、阴性对照组，采用RT-PCR及Western blot检测FoxM1表达变化。采用MTT法、FCM法、Transwell法分别检测下调FoxM1表达对细胞增殖、周期、凋亡、迁移的影响。Western blot检测下调FoxM1表达后β-catenin、C-Myc、Cyclin D1、MMP-2、MMP-9蛋白表达变化。

结果

转染FoxM1-siRNA的5-8F细胞FoxM1 mRNA和蛋白表达水平降低(

＜0.05)。下调FoxM1表达可抑制5-8F细胞增殖(

＜0.05)，FoxM1-siRNA组的凋亡率为(22.68±0.67)%，较阴性对照组[(11.74±1.36)%

]

和空白对照组[(10.94±1.52)%

]

显著增加(

＜0.05)，细胞周期阻滞于G

期。FoxM1-siRNA组细胞穿膜数(100.00±10.97)明显低于阴性对照组(246.00±6.66)和空白对照组(233.70±12.41)，细胞迁移能力降低(

＜0.05)。下调FoxM1的表达可抑制β-catenin细胞核表达，抑制C-Myc、Cyclin D1、MMP-2、MMP-9总蛋白表达(

＜0.001)。

结论

FoxM1表达下调抑制鼻咽癌细胞5-8F的增殖，诱导细胞凋亡，降低细胞迁移能力，使细胞周期阻滞于G

期。其机制可能是通过抑制Wnt通路关键蛋白β-catenin细胞核表达从而抑制Wnt通路活性实现的。

Abstract

Objective

To investigate the effect of siRNA mediated FoxM1 silencing on malignant behaviors of nasopharyngeal carcinoma cell line 5-8F

via

Wnt/β-catenin signaling pathway.

Methods

The FoxM1-siRNA by chemical synthesis was transfected into 5-8F cells

and the blank and negative control cells were set up. Real-time PCR (RT-PCR) and Western blotting were used to detect the mRNA and protein levels of FoxM1 respectively. MTT assay

flow cytometry and Transwell chamber test were used to determine the effect of FoxM1 on proliferation

apoptosis

cell cycle distribution and migration of 5-8F cells. The protein expression levels of β-catenin

C-Myc

Cyclin D1

MMP-2 and MMP-9 were detected by Western blotting.

Results

The mRNA and protein levels of FoxM1 were decreased obviously after FoxM1-siRNA transfection in 5-8F cells (

0.05). When compared with the blank and negative control cells

the silence also inhibited the proliferation (

0.05)

but enhanced apoptotic rate [(22.68±0.67)%

(10.94±1.52)% and (11.74±1.36)%

0.05

]

arrested the cell cycle at G

phase

and decreased the number of invaded cells (100.00±10.97

233.70±12.41 and 246.00±6.66) and cell migration ability (

0.05). Furthermore

Western blotting demonstrated that the nuclear protein level of β-catenin and the total protein levels of C-Myc

Cyclin D1

MMP-2 and MMP-9 were decreased after FoxM1-siRNA transfection (

0.05).

Conclusion

siRNA mediated FoxM1 silencing increases the apoptotic rate

inhibits the proliferation

reduces the migration

and arrests cell cycle at G

phase in nasopharyngeal carcinoma 5-8F cells. Its mechanism may be through suppressing the expression of β-catenin

key protein of Wnt signaling pathway

and thus inhibiting the activation of the pathway.

关键词

Keywords

references

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