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rs2200733多态性与心房颤动、肺静脉大小及其隔离术后复发的相关性研究
Correlation of rs2200733 polymorphism with atrial fibrillation, pulmonary vein size and recurrence after isolation
- 第三军医大学学报 2018年40卷第5期 页码：443-448
- 作者机构：
1.400062 重庆，重庆市第五人民医院心血管内科
2.400010 重庆，重庆医科大学附属第二医院心血管内科
- 作者简介：
[王亚珠，E-mail：274980866@qq.comWANG Yazhu, E-mail: 274980866@qq.com
- 基金信息：
DOI：10.16016/j.1000-5404.201709145
中图分类号： R181.32;R394.1;R541.75收稿：2017-09-15，
修回：2017-12-05，
纸质出版：2018-03-15
- 稿件说明：
移动端阅览
王亚珠, 殷跃辉. rs2200733多态性与心房颤动、肺静脉大小及其隔离术后复发的相关性研究[J]. 第三军医大学学报, 2018,40(5):443-448.
Yazhu WANG, Yuehui YIN. Correlation of rs2200733 polymorphism with atrial fibrillation, pulmonary vein size and recurrence after isolation[J]. Journal of Third Military Medical University, 2018, 40(5): 443-448.
王亚珠, 殷跃辉. rs2200733多态性与心房颤动、肺静脉大小及其隔离术后复发的相关性研究[J]. 第三军医大学学报, 2018,40(5):443-448. DOI： 10.16016/j.1000-5404.201709145.
Yazhu WANG, Yuehui YIN. Correlation of rs2200733 polymorphism with atrial fibrillation, pulmonary vein size and recurrence after isolation[J]. Journal of Third Military Medical University, 2018, 40(5): 443-448. DOI： 10.16016/j.1000-5404.201709145.
摘要
目的
2
研究rs2200733多态性与心房颤动、肺静脉隔离术后复发的相关性。
方法
2
筛选2009年7月至2010年12月期间在重庆地区4家三甲医院心血管内科就诊的患者207例，其中男性114例，女性93例，年龄(60.8±10.7)岁，体质量指数BMI(24.2±3.9)kg/m
2
。按有无心房颤动分为2组：房颤组(有心房颤动，100例)和对照组(无心房颤动病史，107例)。收集患者临床资料和静脉血标本，分别提取其基因组DNA，通过聚合酶链反应-限制性片段长度多态性方法和直接测序法检测各患者的rs2200733多态性的基因型。随访40例行肺静脉隔离术的房颤患者，通过生存分析探讨房颤复发的危险因素。
结果
2
① 房颤组的CT基因型频率(75.0%)较对照组(61.7%)明显增高(
P
＜0.05)。②房颤患者中，CT基因型患者的肺静脉口较TT/CC基因型患者明显增大(
P
＜0.05)。③随访40例行肺静脉隔离术的房颤患者中早期复发11例(27.5%)，晚期复发9例(22.5%)。通过Kaplan-Meier生存分析发现，rs2200733多态性的各种基因型与心房颤动肺静脉隔离术后复发无统计学差异(χ
2
=1.076，
P
>
0.05)；左上肺静脉前后径≥17.45 mm(χ
2
=33.089，
P
＜0.05)、左上肺静脉上下径≥17.50 mm(χ
2
=32.189，
P
＜0.05)、右上肺静脉前后径≥18.60 mm(χ
2
=34.264，
P
＜0.05)、右下肺静脉前后径≥16.30 mm(χ
2
=13.546，
P
＜0.05)、右下肺静脉上下径≥16.70 mm(χ
2
=14.870，
P
＜0.05)是术后复发的危险因素。
结论
2
rs2200733多态性CT基因型可促进房颤患者肺静脉增粗，是房颤发生、发展以及术后复发的危险因子。
Abstract
Objective
2
To investigate the potential association of rs2200733 polymorphism with recurrence of atrial fibrillation after pulmonary vein isolation.
Methods
2
A total of 207 patients admitted in the department of cardiology from 4 Class A Grade 3 hospitals in Chongqing city during July 2009 to December 2010 were recruited in this study. They were 114 males and 93 females
at a mean age of 60.8±10.7 years and a mean BMI of 24.2±3.9 kg/m
2
. They were divided into atrial fibrillation group (
n
=100) and control group (without the history of atrial fibrillation
n
=107). The clinical data were collected. And venous blood samples were harvested for rs2200733 polymorphism by polymerase chain reaction-restriction fragment length p
olymorphism (PCR-RFLP) and gene sequencing. Forty patients undergoing pulmonary vein isolation were followed up to analyze the risk factors for AF recurrence by survival analysis.
Results
2
① The atrial fibrillation group had significant higher genotype frequency of CT than the control group (75.0%
vs
61.7%
P
<
0.05). ② In them
those carrying CT genotype had larger pulmonary vein diameter than the patients with TT/CC (
P
<
0.05). ③ In the 40 patients with follow-up
early recurrence of atrial fibrillation was observed in 11 cases (27.5%)
whereas late recurrence occurred in 9 cases (22.5%). Kaplan-Meier survival analysis revealed that the genotypes of rs2200733 polymorphism had no significant association with atrial fibrillation recurrence after pulmonary vein isolation (
Chi
-square=1.076
P
>
0.05). Front and rear diameter of left superior pulmonary vein larger than 17.45 mm (
Chi
-square=33.089
P
<
0.05)
upper and lower diameter of left superior pulmonary vein larger than 17.50 mm (
Chi
-square=32.189
P
<
0.05)
front and rear diameter of the right superior pulmonary vein larger than 18.60 mm (
Chi
-square=34.264
P
<
0.05)
front and rear diameter of right inferior pulmonary vein larger than 16.30mm (
Chi
-square=13.546
P
<
0.05)
and upper and lower diameter of right inferior pulmonary vein larger than 16.70 mm (
Chi
-square=14.870
P
<
0.05) were the risk factors for AF recurrence.
Conclusion
2
CT genotype in rs2200733 polymorphism may promote pulmonary vein enlargement
and is a risk factor for AF recurrence after pulmonary vein isolation.
关键词
Keywords
references
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