我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析

杨馥嘉; 冯伟; 孙凤军

doi:10.16016/j.1000-5404.201709112

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我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析

临床医学 | 更新时间：2026-01-09

- 我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析
- Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing
- 第三军医大学学报 2018年40卷第3期页码：259-263
- 作者机构：
  
  1.100069 北京，首都医科大学口腔医学系
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院药学部
- 作者简介：
  
  [ 孙凤军，电话：(023)68765991, E-mail: fengj_sun@163.com SUN Fengjun, Tel: 86-23-68765991, E-mail: fengj_sun@163.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201709112
  中图分类号： R181.24;R378.7;R915
- 收稿：2017-09-15，
  
  修回：2017-10-20，
  
  纸质出版：2018-02-15
- 稿件说明：
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杨馥嘉, 冯伟, 孙凤军. 我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析[J]. 第三军医大学学报, 2018,40(3):259-263.

Fujia YANG, Wei FENG, Fengjun SUN. Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing[J]. Journal of Third Military Medical University, 2018, 40(3): 259-263.
杨馥嘉, 冯伟, 孙凤军. 我院呼吸科鲍曼不动杆菌耐药及流行病学特征分析[J]. 第三军医大学学报, 2018,40(3):259-263. DOI： 10.16016/j.1000-5404.201709112.

Fujia YANG, Wei FENG, Fengjun SUN. Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing[J]. Journal of Third Military Medical University, 2018, 40(3): 259-263. DOI： 10.16016/j.1000-5404.201709112.

摘要

目的

探讨呼吸科鲍曼不动杆菌耐药及流行病学特征。

方法

收集2015年1-12月陆军军医大学第一附属医院呼吸科院内感染患者各类临床标本分离的69株鲍曼不动杆菌

通过医院电子病历系统获得患者临床特征

采用琼脂平板倍比稀释法检测细菌最低抑菌浓度

PCR扩增碳青霉烯酶耐药基因

脉冲场凝胶电泳对鲍曼不动杆菌进行基因分型。

结果

呼吸机使用、抗菌药物使用及住院时间是患者感染鲍曼不动杆菌的危险因素(

0.001)。除米诺环素外

鲍曼不动杆菌对其他抗菌药物均具有较高的耐药性。所有菌株含有OXA-51基因

51株携带OXA-23基因

13株携带VIM基因

2株携带IMP-4基因。69株鲍曼不动杆菌共分为9个型和19个亚型

其中D型和H型最为普遍流行。

结论

我院呼吸科鲍曼不动杆菌耐药率较高

具有耐药基因水平传播和小规模暴发流行的特点。

Abstract

Objective

To explore the drug resistance and epidemiological characteristics of isolated

Acinetobacter baumannii

(

A. baumannii

Methods

A total of 69 stains of

A. baumannii

were isolated from the different clinical specimens of nosocomial infectious patients hospitalized in the respiratory department of the First Affiliated Hospital of Army Medical University from January 2015 to December 2015. The clinical data of these patients were obtained from the electronic medical record system of the hospital. The antimicrobial susceptibility was evaluated by agar disk dilution. The carbapenemase resistance genes were amplified by PCR

and the gene types were analyzed by the pulsed-field gel electrophoresis (PFGE).

Results

Mechanical ventilation

prior antibiotics and long hospital stay were the risk factors for

A. baumannii

infection (

0.001). Except for minocycline

our isolated strains showed high drug resistance rates to the other antibiotics. All the isolates carried OXA-51 gene

and 51 isolates had OXA-23 gene. VIM gene presented in 13 isolates

and IMP gene only existed in 2 isolates. The PFGE results showed that the 69 isolates had 9 different genotypes and 19 subtypes

and D and E genotypes were the most popular.

Conclusion

Our isolated

A. baumannii

stains have quite higher resistance rate

and are characterized by horizontal spread of resistance genes and small-scale epidemic outbreak.

关键词

Keywords

references

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