二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积与线粒体融裂基因变化的关系

马鑫; 陈卡; 冉莉; 朱俊东; 糜漫天

doi:10.16016/j.1000-5404.201709101

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二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积与线粒体融裂基因变化的关系

基础医学 | 更新时间：2026-01-09

- 二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积与线粒体融裂基因变化的关系
- Relationship of mitochondrial fusion/fission genes with dihydromyricetin inhibiting liver fat accumulation in high-fat fed mice
- 第三军医大学学报 2018年40卷第1期页码：17-22
- 作者机构：
  
  1.400038 重庆, 第三军医大学军事预防医学院营养与食品安全研究中心, 重庆市营养与食品安全重点实验室, 重庆市医学营养研究中心
  2.610083 成都, 中国人民解放军成都总医院康复医学科
- 作者简介：
  
  [糜漫天, E-mail:mimt@sina.com MI Mantian, E-mail:mimt@sina.com
- 基金信息：
  
  国家自然科学基金面上项目(81372975)
- DOI：10.16016/j.1000-5404.201709101
  中图分类号： R-332;R575.5;R977.6
- 收稿：2017-09-13，
  
  修回：2017-10-17，
  
  纸质出版：2018-01-15
- 稿件说明：
移动端阅览
马鑫, 陈卡, 冉莉, 等. 二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积与线粒体融裂基因变化的关系[J]. 第三军医大学学报, 2018,40(1):17-22.

Xin MA, Ka CHEN, Li RAN, et al. Relationship of mitochondrial fusion/fission genes with dihydromyricetin inhibiting liver fat accumulation in high-fat fed mice[J]. Journal of Third Military Medical University, 2018, 40(1): 17-22.
马鑫, 陈卡, 冉莉, 等. 二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积与线粒体融裂基因变化的关系[J]. 第三军医大学学报, 2018,40(1):17-22. DOI： 10.16016/j.1000-5404.201709101.

Xin MA, Ka CHEN, Li RAN, et al. Relationship of mitochondrial fusion/fission genes with dihydromyricetin inhibiting liver fat accumulation in high-fat fed mice[J]. Journal of Third Military Medical University, 2018, 40(1): 17-22. DOI： 10.16016/j.1000-5404.201709101.

摘要

目的

探讨肝脏线粒体融裂相关基因表达变化在二氢杨梅素抑制高脂喂养小鼠肝脏脂肪蓄积中的可能作用。

方法

45只6周龄C57BL/6J雄性小鼠

按随机数表法分为普通饲料组(CON)

高脂饲料组(HFD)和高脂加二氢杨梅素组(HFD+DHM)

每组15只

干预12周。检测小鼠体质量、肝脏指数、血清生化指标; 分别采用HE染色和油红O染色观察肝细胞脂肪变性和脂滴数量与形态。使用增强型ATP试剂盒检测肝脏ATP含量; 采用荧光定量PCR和Western blot分别检测肝脏线粒体融合与分裂相关基因[动力相关蛋白1(dynamin-related protein 1

Drp1)、分裂蛋白1(fission 1

Fis1)、融合蛋白2(mitofusion 2

Mfn2)、视神经萎缩蛋白1(optic atrophy1

Opa1)]的mRNA及蛋白表达。

结果

与CON组相比

HFD组小鼠体质量、肝脏指数、空腹血糖、空腹胰岛素水平、甘油三酯、总胆固醇、低密度胆固醇明显增高(

0.05)

肝脏ATP含量显著降低(

0.05)

HE染色见明显的肝内脂肪空泡和肝细胞气球样变

油红O

染色见大量的脂滴蓄积

肝脏组织Fis1、Drp1 mRNA和蛋白表达显著增高(

0.05)

而Mfn2、Opa1明显降低(

0.05)。二氢杨梅素干预显著抑制高脂喂养诱导的肝脏指数、空腹血糖、空腹胰岛素和血甘油三酯的升高

肝脏脂肪蓄积以及肝脏ATP含量的降低

并且明显拮抗高脂诱导的线粒体分裂与融合基因mRNA和蛋白的表达水平变化。

结论

二氢杨梅素可明显改善高脂喂养小鼠肝脏的脂肪蓄积

该效应可能与其调节肝脏线粒体的融合与分裂有关。

Abstract

Objective

To investigate the role of the hepatic mitochondrial fusion/fission-related genes in the dihydromyricetin (DHM)-induced inhibition of liver fat accumulation in mice after high-fat diet (HFD).

Methods

Forty-five male C57BL/6J mice (6 weeks old) were randomly divided into control group (CON)

HFD group (HFD) and HFD with DHM treatment (HFD+DHM)

with 15 mice per group.After 12 weeks

the body weight

hepatic index and serum biochemical indexes were detected.HE and oil red O staining were applied to observe the changes of hepatic steatosis and lipid droplets accumulation.The ATP production was measured by an enhanced ATP detecting kit.The mRNA and protein levels of mitochondrial fusion/fission genes

fission 1 (Fis1)

dynamin-related protein 1 (Drp1)

mitofusion 2 (Mfn2) and optic atrophy1 (Opa1) were determined by PCR and Western blotting.

Results

HFD significantly increased body weight

hepatic index

fasting glucose

fasting insulin

triglyceride

total cholesterol and low density lipoprotein (

0.05)

while decreased the ATP production(

0.05).HE staining displayed notable intrahepatic fat vacuoles and hepatocyte balloon-like changes in the liver of HFD mice.And oil red O staining showed more fat droplets in HFD mice.The mRNA and protein levels of hepatic Fis1and Drp1 were significantly increased (

0.05)

while those of Mfn2 and Opa1 were dominantly decreased in HFD group (

0.05).However

DHM treatment could significantly reverse all above

changes induced by HFD(

0.05).

Conclusion

DHM attenuates lipid accumulation in the HFD mice

which may be related to its regulation on mitochondrial fusion/fission functions.

关键词

Keywords

references

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