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口腔鳞癌相关成纤维细胞糖代谢的初步研究
Glucose metabolic alteration in oral carcinoma-associated fibroblasts
- 第三军医大学学报 2018年40卷第1期 页码：53-57
- 作者机构：
637000 四川 南充, 川北医学院口腔医学系
- 作者简介：
[ 刘英, E-mail: 1366937083@qq.com LIU Ying, E-mail: 1366937083@qq.com
- 基金信息：川北医学院博士基金(CBY13-QD-07);川北医学院校级科研发展重点项目(CBY15-A-ZD09)
DOI：10.16016/j.1000-5404.201709090
中图分类号： Q591.4;R730.23;R739.8收稿：2017-09-12，
修回：2017-11-01，
纸质出版：2018-01-15
- 稿件说明：
移动端阅览
唐婉容, 马莹, 喻洁, 等. 口腔鳞癌相关成纤维细胞糖代谢的初步研究[J]. 第三军医大学学报, 2018,40(1):53-57.
Wanrong TANG, Ying MA, Jie YU, et al. Glucose metabolic alteration in oral carcinoma-associated fibroblasts[J]. Journal of Third Military Medical University, 2018, 40(1): 53-57.
唐婉容, 马莹, 喻洁, 等. 口腔鳞癌相关成纤维细胞糖代谢的初步研究[J]. 第三军医大学学报, 2018,40(1):53-57. DOI： 10.16016/j.1000-5404.201709090.
Wanrong TANG, Ying MA, Jie YU, et al. Glucose metabolic alteration in oral carcinoma-associated fibroblasts[J]. Journal of Third Military Medical University, 2018, 40(1): 53-57. DOI： 10.16016/j.1000-5404.201709090.
摘要
目的
2
通过对口腔鳞癌相关成纤维细胞(carcinoma-associate fibroblasts
CAFs)糖代谢相关指标的检测
探讨肿瘤微环境CAFs糖代谢的可能方式并分析其作用和意义。
方法
2
培养CAFs和癌旁正常成纤维细胞(normal fibroblasts
NFs)
詹纳斯绿B染色观察线粒体的数量及分布
JC-1荧光染色观察线粒体膜电位的改变。培养1、2、3、4 d
通过定磷法用可见分光光度计检测细胞中ATP的含量
葡萄糖氧化酶-过氧化物酶法检测细胞摄取葡萄糖的能力
乳酸酶法检测细胞分泌乳酸情况。
结果
2
与NFs组相比
CAFs线粒体詹纳斯绿B和JC-1荧光染色强度明显下降
显示CAFs线粒体活性下降。随着培养时间的延长
CAFs较NFs组摄取葡萄糖增多
ATP产能却减少
分泌乳酸增加
差异均有统计学意义(
P
<
0.05);以培养第4天差异更明显
CAFs和NFs组培养基葡萄糖浓度分别为(10.85±1.42)、(16.04±1.09)mmol/L
产生ATP浓度分别为(42.99±4.64)、(57.37±2.75)μmol/g
产生乳酸浓度分别为(12.1±0.81)、(7.06±0.95)mmol/L。
结论
2
口腔鳞癌CAFs细胞糖代谢方式发生了改变
以糖酵解为主。
Abstract
Objective
2
To explore the possible ways of glucose metabolism in carcinoma-associated fibroblasts (CAFs) by detecting the related indexes of metabolism
and analyze its function and significance in tumor-stroma interplay.
Methods
2
After the CAFs and paracancerous normal fibroblasts (NFs) were cultured
the amount and distribution of mitochondria were observed by Janus green B staining. The mitochondrial membrane potential was tested with specific fluorescent dye JC-1 staining. The phosphomolybdic acid colorimetry was used to measure the ATP content of the cells. Glucose oxidase-peroxidase reagent was used to detect the intracellular glucose level. The change of lactate concentration in 2 groups of cells was detected by lactic acid assay kit.
Results
2
Compared with the NFs group
the mitochondrial activity was lower in the CAFs group
according to the results of Janus green B staining and JC-1 fluorescence staining. As the time elapsed
the ability of glucose-uptaking and lactate secretion were increased in CAFs group
while ATP production was declined
with significant difference when compared with the NFs group (
P
<
0.05). At the fouth day
glucose concentration were 10.85±1.42 mmol/L in the CAFs group and 16.04±1.09 mmol/L in the NFs group
while
ATP concentration was 42.99±4.64 and 57.37±2.75 μmol/g
respectively
and lactic acid concentration were 12.1±0.81 and 7.06±0.95 mmol/L
separately.
Conclusion
2
Oral squamous CAFs have glucose metabolic alteration
mainly through glycolysis.
关键词
Keywords
references
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