软骨细胞SHOX基因过/低表达的差异miRNA筛选与验证

许珂; 徐雪姣; 宋萃; 朱岷

doi:10.16016/j.1000-5404.201709050

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软骨细胞SHOX基因过/低表达的差异miRNA筛选与验证

基础医学 | 更新时间：2026-01-09

- 软骨细胞SHOX基因过/低表达的差异miRNA筛选与验证
- Screening and verification of differential miRNAs in chondrocytes with over-expression and low expression of SHOX
- 第三军医大学学报 2018年40卷第3期页码：211-215
- 作者机构：
  
  400014 重庆，重庆医科大学附属儿童医院内分泌科，儿童发育疾病研究教育部重点实验室，儿童发育重大疾病国家国际科技合作基地, 儿科学重庆市重点实验室
- 作者简介：
  
  [ 朱岷，E-mail: zhumin323@hotmail.com ZHU Min, E-mail: zhumin323@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(81170723)
- DOI：10.16016/j.1000-5404.201709050
  中图分类号： R322.71;R394-33;R394.3
- 收稿：2017-09-07，
  
  修回：2017-10-24，
  
  纸质出版：2018-02-15
- 稿件说明：
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许珂, 徐雪姣, 宋萃, 等. 软骨细胞SHOX基因过/低表达的差异miRNA筛选与验证[J]. 第三军医大学学报, 2018,40(3):211-215.

Ke XU, Xuejiao XU, Cui SONG, et al. Screening and verification of differential miRNAs in chondrocytes with over-expression and low expression of SHOX[J]. Journal of Third Military Medical University, 2018, 40(3): 211-215.
许珂, 徐雪姣, 宋萃, 等. 软骨细胞SHOX基因过/低表达的差异miRNA筛选与验证[J]. 第三军医大学学报, 2018,40(3):211-215. DOI： 10.16016/j.1000-5404.201709050.

Ke XU, Xuejiao XU, Cui SONG, et al. Screening and verification of differential miRNAs in chondrocytes with over-expression and low expression of SHOX[J]. Journal of Third Military Medical University, 2018, 40(3): 211-215. DOI： 10.16016/j.1000-5404.201709050.

摘要

目的

建立软骨细胞(HC-α)SHOX基因过/低表达模型，筛选与未处理组差异表达miRNA，并予以验证。

方法

通过慢病毒过/低表达SHOX基因，用miRNA芯片筛选差异表达的miRNA。

结果

SHOX过表达组与对照组有425条差异表达的miRNA；SHOX低表达组与对照组有379条差异表达的miRNA；按照表达水平差异相反的标准筛选差异miRNA，其中6条miRNA在处理组中表达水平差异相反。实时荧光定量PCR对6条miRNA(miR-126、miR-192、miR-370、miR-432、miR-3162、miR-4745)进行验证，miR-126在过表达组明显上调(

0.05)，在低表达组明显下调(

0.05)。

结论

miR-126参与SHOX介导的软骨细胞凋亡过程。

Abstract

Objective

To establish 2 cell models with overexpression and low expression of short stature homeo-box-containing gene (SHOX) in chondrocytes and screen the differentially expressed miRNAs in the cells.

Methods

Lentiviral vectors were used to establish a cell model of SHOX overexpression and a cell model of SHOX gene interference in HC-α cells. Microarray chip technique was used to screen the differentially expressed miRNAs in the 2 cell models compared with HC-α cells without genetic modification (NC group).

Results

Compared with the NC group

425 and 379 differentially expressed miRNAs

were identified in HC-α cells with SHOX overexpression and in cells with SHOX interference

respectively. Six miRNAs were found to have opposite changes between the 2 cell models

and verification of the 6 miRNAs (miR-126

miR-192

miR-370

miR-432

miR-3162

and miR-4745) with real-time PCR confirmed that miR-126 was significantly up-regulated (

0.05) in cells with SHOX overexpression and was significantly down-regulated (

0.05) in cells with SHOX interference.

Conclusion

miR-126 is involved in SHOX-mediated chondrocyte apoptosis.

关键词

Keywords

references

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