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临床尿路感染分离大肠埃希菌的耐药性及毒力分析
Resistance and virulence of
Escherichia coli isolated from urinary tract infection- 第三军医大学学报 2018年40卷第5期 页码：420-425
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
[夏培元，E-mail:peiyuan_xia2013@163.comXIA Peiyuan, E-mail: peiyuan_xia2013@163.com
- 基金信息：国家自然科学基金面上项目(81373451)
DOI：10.16016/j.1000-5404.201708186
中图分类号： R372;R378.21;R691.3收稿：2017-08-28，
修回：2017-12-12，
纸质出版：2018-03-15
- 稿件说明：
移动端阅览
张镭, 孙凤军, 冯伟, 等. 临床尿路感染分离大肠埃希菌的耐药性及毒力分析[J]. 第三军医大学学报, 2018,40(5):420-425.
Lei ZHANG, Fengjun SUN, Wei FENG, et al. Resistance and virulence of
Escherichia coli isolated from urinary tract infection[J]. Journal of Third Military Medical University, 2018, 40(5): 420-425.张镭, 孙凤军, 冯伟, 等. 临床尿路感染分离大肠埃希菌的耐药性及毒力分析[J]. 第三军医大学学报, 2018,40(5):420-425. DOI： 10.16016/j.1000-5404.201708186.
Lei ZHANG, Fengjun SUN, Wei FENG, et al. Resistance and virulence of
Escherichia coli isolated from urinary tract infection[J]. Journal of Third Military Medical University, 2018, 40(5): 420-425. DOI： 10.16016/j.1000-5404.201708186.
摘要
目的
2
观察急性单纯性和反复性尿路感染大肠埃希菌耐药性及毒力因子的差异，探讨临床尿路感染菌株的致病力。
方法
2
收集本院2014-2015年住院患者临床送检标本，采用平板倍比稀释法检测标本细菌的最低抑菌浓度(minimal inhibitory concentration，MIC)，以细菌平板计数法检测大肠埃希菌的黏附能力，通过细菌的泳动力实验检测大肠埃希菌的运动能力，并用血凝实验检测大肠埃希菌的溶血能力，乳酸脱氢酶实验检测细菌对膀胱癌5637细胞的毒性。
结果
2
共分离出26株大肠埃希菌，其中15株为反复性尿路感染分离菌株(反复性组)，11株为急性单纯性尿路感染分离菌株(急性单纯性组)，毒力结果如下：反复性组黏附能力[(4.95×10
7
±1.21×10
6
)CFU/mL
]
显著强于急性单纯性组[(2.57×10
7
±1.63×10
6
)CFU/mL，
P
＜0.001
]
；反复性组溶血能力(7.30±0.58)也强于急性单纯性组；反复性组菌株对5637细胞的毒性[(76.66±3.65)%
]
显著高于急性单纯性组[(64.14±8.85)%，
P
＝0.019
]
；但两组耐药性、运动能力差异无统计学意义(
P
>
0.05)。
结论
2
反复性尿路感染菌株比急性单纯性尿路感染菌株致病力更强。
Abstract
Objective
2
To investigate the differences in drug resistance and virulence factor of
Escherichia coli
(
E.coli
) strains isolated from the patients with recurrent and acute simple urinary tract infection.
Methods
2
The
clinical samples of the hospitalized patients in our hospital from 2014 to 2015 were collected. Then the minimal inhibitory concentration of the strains was tested by the agar double dilution. The adhesion of the
E. coli
strains was tested with flat colony counting. The motility of
E. coli
was analyzed by the diameter of the zone on the semisolid medium. Type 1 fimbriation was determined by a rabbit erythrocyte hemagglutination for the hemolysis of
E. coli
. The virulence of
E. coli
to the 5637 cells was tested by lactate dehydrogenase test.
Results
2
There were 26 strains of
E. coli
isolated from clinical samples
including 15 from the patients of recurrent and 11 from acute simple urinary tract infection. For the toxicity of the strains
the adhesion ability was 4.95×10
7
±1.21×10
6
CFU/mL for the recurrent isolates and 2.57×10
7
±1.63×10
6
CFU/mL in the acute isolates (
P
<
0.001). The recurrent isolates had stronger hemagglutination (7.3±0.58
vs
6.03±0.73
P
<
0.001)
and stronger toxicity to 5637 cells [(76.66±3.65)%
vs
(64.14±8.85)%
P
＝0.019
]
than the acute isolates. Whereas
there were no significant differences in the resistance and motility of the 2 kinds of isolates (
P
>
0.05).
Conclusion
2
The
E. coli
strains isolated from recurrent urinary tract infections have higher virulent than those from acute simple urinary tract infections.
关键词
Keywords
references
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