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Notch1调控小鼠脑出血后海马神经发生与神经功能恢复
Notch1 regulates hippocampal neurogenesis and recovery of neurological function in mice after intracerebral hemorrhage
- 第三军医大学学报 2017年39卷第24期 页码：2385-2389
- 作者机构：
1.721004 陕西 宝鸡，宝鸡高新人民医院神经内科
2.400037 重庆，第三军医大学新桥医院神经内科
- 作者简介：
[刘娟, E-mail:2824236399@qq.comLIU Juan, E-mail:2824236399@qq.com
- 基金信息：
DOI：10.16016/j.1000-5404.201708143
中图分类号： R338.26; R363.22; R743.34收稿：2017-08-23，
修回：2017-10-16，
纸质出版：2017-12-30
- 稿件说明：
移动端阅览
陈璟, 杨金锁, 罗宝玉, 等. Notch1调控小鼠脑出血后海马神经发生与神经功能恢复[J]. 第三军医大学学报, 2017,39(24):2385-2389.
Jing CHEN, Jinsuo YANG, Baoyu LUO, et al. Notch1 regulates hippocampal neurogenesis and recovery of neurological function in mice after intracerebral hemorrhage[J]. Journal of Third Military Medical University, 2017, 39(24): 2385-2389.
陈璟, 杨金锁, 罗宝玉, 等. Notch1调控小鼠脑出血后海马神经发生与神经功能恢复[J]. 第三军医大学学报, 2017,39(24):2385-2389. DOI： 10.16016/j.1000-5404.201708143.
Jing CHEN, Jinsuo YANG, Baoyu LUO, et al. Notch1 regulates hippocampal neurogenesis and recovery of neurological function in mice after intracerebral hemorrhage[J]. Journal of Third Military Medical University, 2017, 39(24): 2385-2389. DOI： 10.16016/j.1000-5404.201708143.
摘要
目的
2
探讨Notch1在小鼠脑出血(intracerebral hemorrhage，ICH)后对海马神经干细胞(neural stem cells，NSCs)分裂方式影响中的作用以及对小鼠ICH后远期神经功能恢复的影响。
方法
2
利用免疫荧光、Western blot、转轮实验和平衡木实验等方法检测小鼠脑出血后海马NSCs的分裂方式情况、Notch1蛋白表达情况和神经功能恢复情况。
结果
2
与Sham组比较，ICH导致小鼠海马NSCs水平分裂的比例明显降低(
P
＜0.01)，同时发现小鼠海马中Notch1的表达也明显降低(
P
＜0.05)。促进ICH小鼠海马中Notch1的表达能够有效地增加小鼠海马NSCs水平分裂的比例(
P
＜0.01)，维持NSCs的数量，同
时能够有效地增加ICH后小鼠转轮实验掉落的潜伏期以及小鼠海马中DCX阳性细胞数(
P
＜0.01)，维持神经发生的持续性，另外还能够减少ICH后小鼠平衡木行走实验腿的滑落次数(
P
＜0.01)。
结论
2
Notch1信号可以调控脑出血后小鼠海马NSCs的分裂方式以及促进远期神经功能恢复。
Abstract
Objective
2
To investigate the roles of Notch1 in division pattern of hippocampal neural stem cells (NSCs) in mice after intracerebral hemorrhage (ICH)
and its effects on long-term recovery of neurological function.
Methods
2
Immunofluorescence assay
Western blotting
and wheel rotation test and balance beam test were used to detect the division pattern of hippocampal NSCs after ICH
Notch1 protein expression and neurological function recovery
respectively.
Results
2
Compared with the sham group
the rate of horizontal divisions pattern of hippocampal NSCs was decreased significantly in the ICH group (
P
<
0.01). Meanwhile
the expression of Notch1 in the hippocampus was also reduced obviously (
P
<
0.01). While
up-regulation of Notch1 through its activator treatment increased the ratio of horizontal divisions pattern of NSCs and the count of DCX-positive cells in the hippocampus of ICH mice (
P
<
0.01)
maintained the number of NSCs
and effectively prolonged the time on rotarod in wheel rotation test (
P
<
0.01). What's more
the treatment maintained hippocampal neurogenesis and decreased the times of foot faults in balance beam test (
P
<
0.01).
Conclusion
2
Notch1 signal mediates the division pattern of the hippocampal NSCs in mice after ICH and promotes long-term neurological recovery.
关键词
Keywords
references
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