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肝/肾/骨型碱性磷酸酶基因ALPL抑制卵巢癌细胞系SKOV3增殖的研究
Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3
- 第三军医大学学报 2018年40卷第1期 页码：58-63
- 作者机构：
1.400016 重庆, 重庆医科大学:附属第一医院妇产科
2.400016 重庆, 重庆医科大学:基础医学院病理科
- 作者简介：
[ 刘斌, E-mail:my0216@126.com LIU Bin, E-mail: my0216@126.com
- 基金信息：
DOI：10.16016/j.1000-5404.201708130
中图分类号： R394.2;R730.23;R737.31收稿：2017-08-21，
修回：2017-10-19，
纸质出版：2018-01-15
- 稿件说明：
移动端阅览
程李娟, 詹诗杰, 刘斌, 等. 肝/肾/骨型碱性磷酸酶基因ALPL抑制卵巢癌细胞系SKOV3增殖的研究[J]. 第三军医大学学报, 2018,40(1):58-63.
Lijuan CHENG, Shijie ZHAN, Bin LIU, et al. Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3[J]. Journal of Third Military Medical University, 2018, 40(1): 58-63.
程李娟, 詹诗杰, 刘斌, 等. 肝/肾/骨型碱性磷酸酶基因ALPL抑制卵巢癌细胞系SKOV3增殖的研究[J]. 第三军医大学学报, 2018,40(1):58-63. DOI： 10.16016/j.1000-5404.201708130.
Lijuan CHENG, Shijie ZHAN, Bin LIU, et al. Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3[J]. Journal of Third Military Medical University, 2018, 40(1): 58-63. DOI： 10.16016/j.1000-5404.201708130.
摘要
目的
2
研究肝/肾/骨型碱性磷酸酶基因(alkaline phosphatase
liver/bone/kidney
ALPL)在卵巢癌细胞株中的表达及其对卵巢癌细胞SKOV3增殖的影响。
方法
2
通过qRT-PCR检测ALPL在不同卵巢癌细胞株(HEY、SKOV3、8910、ES-2、OVCAR3、3AO、A2780) mRNA中的表达
并与正常卵巢组织进行比较。在低表达ALPL的卵巢癌细胞株SKOV3中外源性过表达ALPL
通过MTT法、平板克隆实验检测细胞的增殖
流式细胞仪检测细胞周期。通过建立裸鼠皮下种植瘤模型
观察过表达ALPL的SKOV3细胞在体内的成瘤能力。
结果
2
ALPL在卵巢癌细胞中的mRNA表达低于正常卵巢组织(
P
<
0.01)
外源性过表达ALPL可抑制SKOV3细胞的增殖和克隆形成率(
P
<
0.01)
阻滞细胞G
1
期向S的转化
并抑制肿瘤细胞的成瘤能力(
P
<
0.05)。
结论
2
ALPL表达的恢复可以明显抑制卵巢癌细胞SKOV3的增殖及成瘤能力。
Abstract
Objective
2
To investigate the expression of tissue-nonspecific alkaline phosphatase
liver/bone/kidney (ALPL) in human ovarian cancer cell lines
and determine its effect on the proliferation of ovarian cancer SKOV3 cells.
Methods
2
qRT-PCR was used to detect the expression of ALPL mRNA level in 7 ovarian cancer cell lines (HEY
SKOV3
8910
ES-2
OVCAR3
3AO and A2780). The results were compared with its level in normal ovarian tissues. After the SKOV3 cells (with low expression of ALPL) were interfered with lentiviral vector to induce ALPL overexpression
cell proliferation was measured by MTT assay and plate colony formation assay
and cell cycle were assessed by flow cytometry. Tumorigenesis was evaluated through xenograft growth of ALPL-overexpressed SKOV3 cells in nude mice.
Results
2
The expression level of ALPL was significantly lower in all the ovarian cancer cell lines studied when compared with that in normal ovarian tissues (
P
<
0.01). ALPL Over-expression resulted in the decreases in cell proliferation and clone formation rate (
P
<
0.01) and blocked the transition of G
1
phase to S phase in SKOV3 cells
as well as suppressed the subcutaneous tumorigenesis in xenografts tumor model (
P
<
0.05).
Conclusion
2
Reversion of ALPL expression level might obviously suppress the cell proliferation and tumorigenesis of SKOV3 cells.
关键词
Keywords
references
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