重组人MG53对CCl<sub>4</sub>诱导的急性肝损伤的保护作用

周中淑; 巩正藩; 杨东海; 吴连判; 夏天洋; 符金娟; 刘渔凯; 韩愈; 曾春雨

doi:10.16016/j.1000-5404.201708123

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重组人MG53对CCl₄诱导的急性肝损伤的保护作用

基础医学 | 更新时间：2026-01-09

- 重组人MG53对CCl₄诱导的急性肝损伤的保护作用
- Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice
- 第三军医大学学报 2017年39卷第23期页码：2270-2275
- 作者机构：
  
  400042 重庆，第三军医大学大坪医院野战外科研究所心血管内科，重庆市心血管病研究所
- 作者简介：
  
  [曾春雨，E-mail:chunyuzeng01@163.com ZENG Chunyu, E-mail:chunyuzeng01@163.com
- 基金信息：
  
  国家自然科学基金青年科学基金(81600585)
- DOI：10.16016/j.1000-5404.201708123
  中图分类号： R322.47;R341;R575
- 收稿：2017-08-20，
  
  修回：2017-09-28，
  
  纸质出版：2017-12-15
- 稿件说明：
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周中淑, 巩正藩, 杨东海, 等. 重组人MG53对CCl₄诱导的急性肝损伤的保护作用[J]. 第三军医大学学报, 2017,39(23):2270-2275.

Zhongshu ZHOU, Zhengfan GONG, Donghai YANG, et al. Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice[J]. Journal of Third Military Medical University, 2017, 39(23): 2270-2275.
周中淑, 巩正藩, 杨东海, 等. 重组人MG53对CCl₄诱导的急性肝损伤的保护作用[J]. 第三军医大学学报, 2017,39(23):2270-2275. DOI： 10.16016/j.1000-5404.201708123.

Zhongshu ZHOU, Zhengfan GONG, Donghai YANG, et al. Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice[J]. Journal of Third Military Medical University, 2017, 39(23): 2270-2275. DOI： 10.16016/j.1000-5404.201708123.

摘要

目的

研究重组人MG53(rhMG53)对四氯化碳(CCl

)诱导的急性肝损伤的保护作用及其相关机制。

方法

40只雄性C57小鼠采用完全随机化分组法分成4组:正常对照组、rhMG53组、CCl

组、rhMG53+CCl

组，每组10只。CCl

组、rhMG53+CCl

组小鼠予0.1% CCl

花生油溶液2 mL/kg腹腔内注射，rhMG53+CCl

组在注射CCl

前30 min予rhMG53 1 mg/kg肌肉注射。rhMG53组予rhMG53 1 mg/kg肌肉注射。正常进食进水24 h后眼球取血分离血清，测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性。取小鼠肝脏

观察肝脏的组织学改变。以体外培养的人肝癌细胞(HepG2)为靶细胞，观察rhMG53对CCl

诱导的细胞损伤的保护作用。

结果

rhMG53降低小鼠血清ALT、AST、LDH水平，病理镜检显示rhMG53可改善肝脏的病理结构，差异均有统计学意义(

0.05)；rhMG53可以修复HepG2细胞膜，保护CCl

对HepG2细胞的损伤。

结论

rhMG53改善CCl

诱导的急性肝细胞损伤，保护肝脏功能。

Abstract

Objective

To determine the protective effect of recombinant human MG53 (rhMG53) on acute liver injury in mice induced by carbon tetrachloride (CCl

)

and investigate its underlying mechanism.

Methods

Male C57 mice (

=40) were randomly divided into 4 groups (

=10)

that is

normal control group

rhMG53 group

CCl

group

and rhMG53+CCl

group. The animals of the CCl

group and rhMG53+CCl

group were injected intraperitoneally with 0.1% CCl

peanut oil solution at a dose of 2 mL/kg

and the mice of the latter group were injected intramuscularly with 1 mg/kg rhMG53 in 0.5 h before

CCl

injection. In 24 h after normal feeding

blood samples were collected after eyeball extirpating

and the serum levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured. The liver tissues of the mice were also harvested for histological changes of the livers. Human hepatocellular carcinoma cells (HepG2) were used as target cells to observe the protective effect of rhMG53 on CCl

-induced cell injury.

Results

RhMG53 treatment reduced serum ALT

AST and LDH levels

and significant attenuated the pathological injuries induced by CCl

(

0.05). rhMG53 repaired cell membrane and reduced CCl

-induced damage to HepG2 cells.

Conclusions

rhMG53 shows protective effect on CCl

-induced acute hepatocellular injury and protects the liver function in mice.

关键词

Keywords

references

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Protective effect of recombinant human mitsugumin 53 on CCl4-induced acute liver injury in mice

DOI：10.16016/j.1000-5404.201708123

摘要

Abstract

关键词

Keywords

references

重组人MG53对CCl₄诱导的急性肝损伤的保护作用

Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice