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尿足细胞数量及podocalyxin蛋白与IgA肾病的相关性
Correlation of urinary podocyte count and podocalyxin level with IgA nephropathy
- 第三军医大学学报 2017年39卷第24期 页码：2406-2411
- 作者机构：
1.710021 西安, 西安医学院临床医学院
2.710043 西安，陕西省第四人民医院肾病内科
- 作者简介：
[尚瑜，E-mail：doctorsun2@126.comSHANG Yu, E-mail:doctorsun2@126.com
- 基金信息：陕西省2016年度卫生科研项目(2016D021)
DOI：10.16016/j.1000-5404.201707200
中图分类号： R181.21;R446.12;R692.31收稿：2017-08-01，
修回：2017-10-16，
纸质出版：2017-12-30
- 稿件说明：
移动端阅览
尚瑜, 谭峰. 尿足细胞数量及podocalyxin蛋白与IgA肾病的相关性[J]. 第三军医大学学报, 2017,39(24):2406-2411.
Yu SHANG, Feng TAN. Correlation of urinary podocyte count and podocalyxin level with IgA nephropathy[J]. Journal of Third Military Medical University, 2017, 39(24): 2406-2411.
尚瑜, 谭峰. 尿足细胞数量及podocalyxin蛋白与IgA肾病的相关性[J]. 第三军医大学学报, 2017,39(24):2406-2411. DOI： 10.16016/j.1000-5404.201707200.
Yu SHANG, Feng TAN. Correlation of urinary podocyte count and podocalyxin level with IgA nephropathy[J]. Journal of Third Military Medical University, 2017, 39(24): 2406-2411. DOI： 10.16016/j.1000-5404.201707200.
摘要
目的
2
探讨尿足细胞数量和podocalyxin蛋白表达与IgA肾病发病、发展的相关性，探索IgA肾病的诊断、病情和预后评估提供的新指标。
方法
2
采用间接免疫荧光法检测陕西省第四人民医院体检的健康人群和150例IgA肾病患者尿足细胞数量及podocalyxin蛋白表达水平，观察健康人群和IgA肾病患者尿足细胞数量差异和不同肾脏病理Lee分级IgA肾病患者尿足细胞数量和podocalyxin蛋白表达水平差异，分析不同肾脏病理Lee分级IgA肾病患者尿足细胞数量和podocalyxin蛋白表达与肾小球滤过率、24 h尿蛋白定量、血清肌酐、尿素氮等临床指标的相关性。
结果
2
健康人群组尿沉渣细胞涂片染色标本未见尿足细胞，而IgA肾病组尿沉渣细胞涂片染色标本尿足细胞数量为每20个高倍视野(12.54±2.89)；不同肾脏病理Lee分级IgA肾病患者的尿足细胞数量、podocalyxin蛋白表达和24 h尿蛋白定量比较，差异有统计学意义(
P
＜0.05)，且不同肾脏病理Lee分级IgA肾病患者尿足细胞数量与24 h尿蛋白定量呈正相关(
r
=0.835 3
P
＜0.05)，podocalyxin蛋白表达与24 h尿蛋白定量呈负相关(
r
=0.886 8
P
＜0.05)。
结论
2
尿足细胞数量、podocalyxin蛋白表达与IgA肾病的发病、发展密切相关。
Abstract
Objective
2
To investigate the correlation of the number of urinary podocytes and the expression level podocalyxin with the occurrence and development of IgA nephropathy so as to explore new markers for the assessment of the diagnosis
condition and prognosis of the disease.
Methods
2
A total of 150 patients with identified IgA nephropathy admitted in Fourth People's Hospital of Shaanxi Province in 2016 were enrolled in this study and assigned into observation group. Another 150 sex-and age-matched healthy individuals who took physical examination in the same hospital during the same period served as control group. Indirect immunofluorescence assay was used to measure the number of podocytes and level of podocalyxin protein in the urinary samples from the observation and control groups. The results were compared between the 2 groups
among the IgA nephropathy patients with different grades of kidney pathology Lee classification. The correlations of urinary podocyte numbers and podocalyxin levels in the patients with different Lee grades were analyzed with the glomerular filtration rate
24 h urinary protein
serum creatinine and urea nitrogen.
Results
2
No podocytes were observed in the urine sediment smear from the healthy individuals
and the number was 12.54±2.89 (per 20 high power field) in the observation group. Significant differences were found in the podocyte number
podocalyxin protein level
24 h urinary protein among the patients at different Lee grades (
P
<
0.05). In the patients of different Lee grades
the urinary podocyte count was positively correlated with the 24 h urine protein(
r
=0.835 3
P
<
0.05)
while podocalyxin protein expression was negatively correlated with 24 h urine protein(
r
=0.-0.886 8
P
<
0.05).
Conclusion
2
Urinary podocyte count and podocalyxin protein level are closely related to the occurrence and development of IgA nephropathy.
关键词
Keywords
references
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