水通道蛋白9在2型糖尿病大鼠肝脏中的表达变化

李原; 侯娇娇; 任旭霞; 陈玉琴; 易耀兴; 杨美

doi:10.16016/j.1000-5404.201707189

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水通道蛋白9在2型糖尿病大鼠肝脏中的表达变化

基础医学 | 更新时间：2026-01-09

- 水通道蛋白9在2型糖尿病大鼠肝脏中的表达变化
- Expression profile of AQP9 in liver of rats with type 2 diabetes mellitus
- 第三军医大学学报 2018年40卷第1期页码：31-36
- 作者机构：
  
  1.400016 重庆, 重庆医科大学:神经科学中心
  2.400016 重庆, 重庆医科大学:第一临床学院2013级临床医学七年制
- 作者简介：
  
  [杨美, E-mail:yangmei19802002@126.com YANG Mei, E-mail:yangmei19802002@126.com
- 基金信息：
  
  国家自然科学基金面上项目(81671312);国家自然科学基金面上项目(81000566);重庆市自然科学基金(CSTC2015jcyA10018);重庆医科大学基础医学院苗圃基金(JC201506);重庆医科大学大学生科学研究与创新实验(201502)
- DOI：10.16016/j.1000-5404.201707189
  中图分类号： R322.47;R341;R587.1
- 收稿：2017-07-27，
  
  修回：2017-11-15，
  
  纸质出版：2018-01-15
- 稿件说明：
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李原, 侯娇娇, 任旭霞, 等. 水通道蛋白9在2型糖尿病大鼠肝脏中的表达变化[J]. 第三军医大学学报, 2018,40(1):31-36.

Yuan LI, Jiaojiao HOU, Xuxia REN, et al. Expression profile of AQP9 in liver of rats with type 2 diabetes mellitus[J]. Journal of Third Military Medical University, 2018, 40(1): 31-36.
李原, 侯娇娇, 任旭霞, 等. 水通道蛋白9在2型糖尿病大鼠肝脏中的表达变化[J]. 第三军医大学学报, 2018,40(1):31-36. DOI： 10.16016/j.1000-5404.201707189.

Yuan LI, Jiaojiao HOU, Xuxia REN, et al. Expression profile of AQP9 in liver of rats with type 2 diabetes mellitus[J]. Journal of Third Military Medical University, 2018, 40(1): 31-36. DOI： 10.16016/j.1000-5404.201707189.

摘要

目的

探讨水通道蛋白9(Aquaporin-9

AQP9)在2型糖尿病(T2DM)鼠肝脏中的表达情况及其可能的调控机制。

方法

采用高脂高糖饲养联合低剂量链脲霉素诱导T2DM模型; 通过HE染色法检测不同组SD大鼠肝脏的形态学改变; 免疫荧光染色法观察不同组大鼠肝脏中AQP9的分布情况; Western blot检测不同组大鼠肝脏中AQP9和MAPK蛋白水平的表达变化。

结果

相较于对照组

HE染色显示

2型糖尿病鼠的肝脏细胞排列混乱

细胞间隙增宽

细胞水样变性显著

水肿明显

伴随大量炎性细胞浸润; 免疫荧光显示

水通道蛋白9在对照组表达较少

而在糖尿病组大量表达

主要呈环形集中分布在肝细胞朝向肝血窦面的细胞膜上; Western blot分析表明

糖尿病组肝脏AQP9的表达水平明显升高(

＜0.05)

p-JNK/JNK的表达升高

(

＜0.05)

p-p38/p38的表达下降(

＜0.05)

p-ERK/ERK的变化差异无统计学意义(

＞0.05)。

结论

AQP9的表达水平在T2DM鼠肝脏中显著上升

高糖环境下p38-MAPK

JNK-MAPK可能对AQP9的表达具备调控作用。

Abstract

Objective

To investigate the expression profile of aquaporin-9 (AQP9) in the liver of type 2 diabetes mellitus (T2DM) rats and explore the possible regulatory mechanism.

Methods

Male adult Sprague-Dawley rats were fed with high fat diet and given an intraperitoneal injection of streptozotocin (STZ) to induce T2DM model.Hematoxylin-eosin (HE) staining was used to observe the morphological changes in the liver.Immunofluorescence assay was employed to investigate the distribution of AQP9 in the liver.Western blotting was adopted to detect the hepatic expression of AQP9 at protein level.

Results

Compared with the control group

the hepatic cells in the T2DM rats were swollen

with hydropic degeneration

and in disorganized arrangement

and some inflammatory cells had infiltrated in the liver tissue.Immunofluorescent microscopy showed that AQP9 was rarely expressed in the liver of control rats

but massively expressed in the liver of T2DM rats

mainly concentrating in rings in the cell membrane towards hepatic sinusoid.Western blotting demonstrated that the expression level of AQP9 was significantly increased (

0.05)

and the ratio of p-p38 to p38 was obviously reduced (

0.05)

and that of p-JNK to JNK notably increased (

0.05)

while that of p-ERK to ERK had no change (

0.05).

Conclusion

The expression of AQP9 is obviously increased in the liver of T2DM rats.p38-MAPK and JNK-MAPK signal pathways may play regulatory role in its expression in hyperglycemic condition.

关键词

Keywords

references

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