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负载抗sca-1抗体和碱性成纤维细胞生长因子的脱细胞膀胱基质的生物性能研究
Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor
- 第三军医大学学报 2017年39卷第22期 页码：2176-2181
- 作者机构：
400038 重庆，第三军医大学西南医院妇产科
- 作者简介：
[ 徐惠成, E-mail: 541289314@qq.com XU Huicheng, E-mail: 541289314@qq.com
- 基金信息：国家自然科学基金面上项目(81471443)
DOI：10.16016/j.1000-5404.201707156
中图分类号： R318.08;R322.62;R329.28收稿：2017-07-24，
修回：2017-08-18，
纸质出版：2017-11-30
- 稿件说明：
移动端阅览
李建奎, 梁志清, 徐惠成. 负载抗sca-1抗体和碱性成纤维细胞生长因子的脱细胞膀胱基质的生物性能研究[J]. 第三军医大学学报, 2017,39(22):2176-2181.
Jiankui LI, Zhiqing LIANG, Huicheng XU. Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor[J]. Journal of Third Military Medical University, 2017, 39(22): 2176-2181.
李建奎, 梁志清, 徐惠成. 负载抗sca-1抗体和碱性成纤维细胞生长因子的脱细胞膀胱基质的生物性能研究[J]. 第三军医大学学报, 2017,39(22):2176-2181. DOI： 10.16016/j.1000-5404.201707156.
Jiankui LI, Zhiqing LIANG, Huicheng XU. Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor[J]. Journal of Third Military Medical University, 2017, 39(22): 2176-2181. DOI： 10.16016/j.1000-5404.201707156.
摘要
目的
2
观察负载抗sca-1抗体(anti-sca-1)和碱性成纤维细胞生长因子(basic fibroblast growth factor
bFGF)-脱细胞膀胱基质(urinary bladder matrix
UBM)生物支架的生物学性能，探讨其作为新型盆底修复材料的可能性。
方法
2
通过双特异性化学交联试剂4-(N-马来酰亚胺甲基)环己烷-1-羧酸磺酸基琥珀酰亚胺酯钠盐(sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate
sulfo-smcc)将anti-sca-1和bFGF特异性结合到UBM上，得到anti-sca-1/bFGF-UBM生物支架。通过扫描电子显微镜观察生物支架表面结构，CCK-8(cell counting kit-8)间接检测体外富集sca-1阳性细胞情况，免疫荧光观察支架诱导小鼠骨髓间充质干细胞(mouse mesenchymal stem cells，mMSCs)分化成熟情况。
结果
2
扫描电子显微镜显示anti-sca-1和bFGF能特异性的结合到UBM上。anti-sca-1和bFGF组荧光强度明显高于对照组(
P
<
0.05)。anti-sca-1/bFGF-UBM生物支架富集的sca-1阳性细胞明显较对照组多(
P
<
0.05)。免疫荧光观察结果提示anti-sca-1/bFGF-UBM生物支架可以促进mMSCs向平滑肌细胞分化。
结论
2
anti-sca-1/bFGF-UBM生物支架显示出了良好的生物性能，既能富集sca-1阳性细胞，又能进一步促进细胞分化成熟。
Abstract
Objective
2
To evaluate the biocompatibility of porcine urinary bladder matrix (UBM) loaded with anti-sca-1 antibody and basic fibroblast growth factor (bFGF) and test its feasibility as a new material for repairing pelvic prolapse.
Methods
2
A bispecific cross-linking reagent sulfosuccinimidyl 4-[N-maleimidomethyl
]
cyclohexane-1-carboxylate (sulfo-smcc) was used to bind anti-sca-1 and bFGF to the UBM to obtain the anti-sca-1/bFGF-UBM biological scaffold. The structure of this scaffold material was observed using scanning electron microscopy
and its performance in enriching sca-1-positive cells
in vitro
was evaluated using cell counting kit-8 (CCK-8) assay. Immunofluorescence assay was employed to assess the differentiation of mouse mesenchymal stem cells (mMSCs) seeded in this scaffold material.
Results
2
Scanning electron microscopy showed that anti-sca-1 and bFGF could specifically bind to the UBM. The anti-sca-1/bFGF-UBM biological scaffold exhibited a significantly stronger fluorescence intensity than the control material (
P
<
0.05) and effectively enriched sca-1-positive cells
in vitro
(
P
<
0.05). Immunofluorescence assay confirmed that mMSCs seeded in this scaffold material were capable of differentiating into smooth muscle cells.
Conclusion
2
The anti-sca-1/bFGF-UBM scaffold shows a good biocompatibility.
关键词
Keywords
references
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