软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损

庞懿; 罗江明; 黄杨; 郭林; 杨柳

doi:10.16016/j.1000-5404.201705081

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软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损

基础医学 | 更新时间：2026-01-09

- 软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损
- Effect of articular chondrocytes combined with sodium alginate hydrogel sphere on rat calvarial defects
- 第三军医大学学报 2017年39卷第20期页码：1993-1997
- 作者机构：
  
  400038 重庆, 第三军医大学西南医院关节外科中心
- 作者简介：
  
  [郭林, E-mail: guolin6212@163.com GUO Lin, E-mail: guolin6212@163.com
  [杨柳, E-mail: jointsurgery@163.com YANG Liu, E-mail: jointsurgery@163.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201705081
  中图分类号： R322.491;R329.24;R979.1
- 收稿：2017-05-23，
  
  修回：2017-06-22，
  
  纸质出版：2017-10-30
- 稿件说明：
移动端阅览
庞懿, 罗江明, 黄杨, 等. 软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损[J]. 第三军医大学学报, 2017,39(20):1993-1997.

Yi PANG, Jiangming LUO, Yang HUANG, et al. Effect of articular chondrocytes combined with sodium alginate hydrogel sphere on rat calvarial defects[J]. Journal of Third Military Medical University, 2017, 39(20): 1993-1997.
庞懿, 罗江明, 黄杨, 等. 软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损[J]. 第三军医大学学报, 2017,39(20):1993-1997. DOI： 10.16016/j.1000-5404.201705081.

Yi PANG, Jiangming LUO, Yang HUANG, et al. Effect of articular chondrocytes combined with sodium alginate hydrogel sphere on rat calvarial defects[J]. Journal of Third Military Medical University, 2017, 39(20): 1993-1997. DOI： 10.16016/j.1000-5404.201705081.

摘要

目的

探讨软骨细胞-海藻酸钠水凝胶修复骨缺损的可行性，比较其与单纯海藻酸钠水凝胶修复效果的差别。

方法

取SD大鼠乳鼠膝关节软骨分离培养获得第2代软骨细胞，以1×10

/mL接种于海藻酸钠水溶液中培养并交联形成复合水凝胶微球。取12只2月龄雄性SD大鼠，建立颅顶单侧缺损模型，随机分为实验组与对照组，分别植入软骨细胞-海藻酸钠水凝胶和单纯海藻酸钠水凝胶微球。于术后6周取材行大体及组织学观察以及Micro-CT扫描定量分析。

结果

大体观察示术后6周实验组缺损已基本修复完全，表面光滑无塌陷或碎裂，质地较硬接近新生骨组织；对照组缺损明显，周围为不规则骨和纤维结缔样组织。Micro-CT扫描示实验组颅骨缺损得到良好的修复重建，定量分析示实验组骨矿物质密度、骨组织体积分数及骨小梁数目均高于对照组，差异有统计学意义(

0.05)。组织学观察示术后6周实验组缺损区由新生骨组织修复，含少量软骨及纤维结缔组织，出现血管结构；对照组缺损明显，边缘主要由纤维结缔组织和少量新生骨组织构成。

结论

海藻酸钠复合软骨细胞制备的水凝胶微球对于大鼠颅骨缺损具有一定的修复效果。

Abstract

Objective

To explore the feasibility of sodium alginate hydrogel with articular chondrocytes in the repair of calvarial defects in rats

and compare its therapeutic effects with simple sodium alginate hydrogel.

Methods

Chondrocytes were isolated from the knee-joint of Sprague Dawley (SD) rat pups and expanded

in vitro

and then the cells of passage 2 (1×10

/mL) were inoculated in sodium alginate and crosslinked to be hydrogel sphere. A total of 12 male rats (2 months old) were randomly divided into control and experiment groups. After a circular defect of 5 mm in diameter was inflicted on one side of rat calvarial bone

the composite of chondrocytes and sodiun alginate hydrogel sphere were implanted into the defect in the rats of the experiment group

while only sodium alginate hydrogel sphere were implanted into the defect of the control group. In 6 weeks after operation

all specimens were assessed with general observation

histological analysis and micro-computed tomography (Micro-CT) to evaluate the bone regeneration.

Results

In 6 weeks after operation

general observation showed that the defects almost have been repaired completely in the experiment group

with the surface smooth without collapses or segments

and hard and dense texture. But the defects were obvious and irregular in the control group

even with fibrous connective tissue around. The Micro-CT showed good repairs and reconstruction of cranial bone in the experiment group

and quantification analysis of relevant parameters showed bone mineral density

bone volume fraction and number of trabecular bones were greater in the experiment group than the control group (

0.05). Histological results showed that the defects in the experiment group were repaired with fresh bone tissues and less cartilage or collagen fibers

even blood capillaries were observed at the same time. The defects of group B were almost vacant with fibrous connective tissues and less bone tissues on the edge.

Conclusion

The articular chondrocytes combined with sodium alginate hydrogel sphere can reconstruct cranial bone in calvarial defects of SD rats.

关键词

Keywords

references

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